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microarray hybridization chamber kit  (Agilent technologies)


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    Structured Review

    Agilent technologies microarray hybridization chamber kit
    Microarray Hybridization Chamber Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+chambers/pmc10127108-156-15-19
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Cosmetic or dermatological composition and use thereof
    Article Snippet: Briefly, reverse transcription and amplification of the total RNAs into cDNA and then into cRNA and labeling thereof with cyanine 3 are performed using the Low Input Quick Amp Labeling Kit (Agilent Technologies). .. Purification of the cRNAs was performed using the RNeasy mini kit (Qiagen, Hilden, Germany) and hybridization with the Microarray hybridization chamber kit (Agilent Technologies, Santa Clara, USA). .. The microarrays were then scanned using the SureScan scanner (Agilent Technologies, Santa Clara, USA) and the Scan control software (Agilent Technologies, Santa Clara, USA).

    Hybridization:

    Article Title: Cosmetic or dermatological composition and use thereof
    Article Snippet: Briefly, reverse transcription and amplification of the total RNAs into cDNA and then into cRNA and labeling thereof with cyanine 3 are performed using the Low Input Quick Amp Labeling Kit (Agilent Technologies). .. Purification of the cRNAs was performed using the RNeasy mini kit (Qiagen, Hilden, Germany) and hybridization with the Microarray hybridization chamber kit (Agilent Technologies, Santa Clara, USA). .. The microarrays were then scanned using the SureScan scanner (Agilent Technologies, Santa Clara, USA) and the Scan control software (Agilent Technologies, Santa Clara, USA).

    Article Title: Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistant Saccharomyces cerevisiae obtained by evolutionary engineering
    Article Snippet: Internal controls were provided by Agilent One-Color RNA Spike-In Kit. cRNA samples were labeled with cyanine 3 (Cy3), and purified using Absolutely RNA Nanoprep Kit (Agilent). .. Hybridization of the samples on microarray slides was done at 65°C for 17 h, using Microarray Hybridization Chamber Kit (Agilent). .. Following washing steps, the microarray slides were scanned, and the data were analyzed by using Agilent Feature Extraction Software (Version 10.7) and GeneSpring GX Data Analysis Software (Version 12.0).

    Article Title: Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistant Saccharomyces cerevisiae obtained by evolutionary engineering.
    Article Snippet: Internal controls were provided by Agilent One-Color RNA Spike-In Kit. cRNA samples were labeled with cyanine 3 (Cy3), and purified using Absolutely RNA Nanoprep Kit (Agilent). .. Hybridization of the samples on microarray slides was done at 65°C for 17 h, using Microarray Hybridization Chamber Kit (Agilent). .. Following washing steps, the microarray slides were scanned, and the data were analyzed by using Agilent Feature Extraction Software (Version 10.7) and GeneSpring GX Data Analysis Software (Version 12.0).

    Article Title: A microRNA profile in Fmr1 knockout mice reveals microRNA expression alterations with possible roles in fragile X syndrome
    Article Snippet: MicroRNA microarrays were carried out using miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (5190-0456) by Agilent Technologies ( http://www.agilent.com ). .. Oligonucleotide arrays were printed with trimer oligonucleotide probes (antisense to miRNAs) specific for 1080 mouse miRNAs using Agilent Microarray Hybridization Chamber Kit (G2534A), and miRNA expression profiling was performed and analyzed as previously described ( ; ). .. Briefly, total RNAs were isolated from the hippocampus of 3 Fmr1 KO mice and 3 WT mice using Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions.

    Article Title: Loss of Mitochondrial Functions Associated with Azole Resistance in Candida glabrata Results in Enhanced Virulence in Mice
    Article Snippet: A total of 600 ng of Cy3-labeled cRNAs from each sample in a volume of 40 μl was fragmented and hybridized for 17 h at 65°C to each array using the Gene Expression Hybridization kit (Agilent Technologies) according to the manufacturer's instructions. .. We used a Microarray Hybridization Chamber kit (Agilent Technologies) and a gasket slide with a format of eight microarrays per slide for sample hybridization to separate each sample in specific subarrays of the 8 × 15K format. ..

    Article Title: Functional Characterization of Aspergillus nidulans ypkA, a Homologue of the Mammalian Kinase SGK
    Article Snippet: The fragmentation was interrupted by adding 55 μL of 2X GE Hybridization Buffer HI-RPM. .. Finally, 100 µL of sample was placed down onto the microarray slide, which was mounted into the Agilent™ Microarray Hybridization Chamber Kit. .. The hybridization was carried out in an oven (Agilent G2545A Hybridization Oven) set to 65°C for 17 hours.

    Microarray:

    Article Title: Cosmetic or dermatological composition and use thereof
    Article Snippet: Briefly, reverse transcription and amplification of the total RNAs into cDNA and then into cRNA and labeling thereof with cyanine 3 are performed using the Low Input Quick Amp Labeling Kit (Agilent Technologies). .. Purification of the cRNAs was performed using the RNeasy mini kit (Qiagen, Hilden, Germany) and hybridization with the Microarray hybridization chamber kit (Agilent Technologies, Santa Clara, USA). .. The microarrays were then scanned using the SureScan scanner (Agilent Technologies, Santa Clara, USA) and the Scan control software (Agilent Technologies, Santa Clara, USA).

    Article Title: Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistant Saccharomyces cerevisiae obtained by evolutionary engineering
    Article Snippet: Internal controls were provided by Agilent One-Color RNA Spike-In Kit. cRNA samples were labeled with cyanine 3 (Cy3), and purified using Absolutely RNA Nanoprep Kit (Agilent). .. Hybridization of the samples on microarray slides was done at 65°C for 17 h, using Microarray Hybridization Chamber Kit (Agilent). .. Following washing steps, the microarray slides were scanned, and the data were analyzed by using Agilent Feature Extraction Software (Version 10.7) and GeneSpring GX Data Analysis Software (Version 12.0).

    Article Title: Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistant Saccharomyces cerevisiae obtained by evolutionary engineering.
    Article Snippet: Internal controls were provided by Agilent One-Color RNA Spike-In Kit. cRNA samples were labeled with cyanine 3 (Cy3), and purified using Absolutely RNA Nanoprep Kit (Agilent). .. Hybridization of the samples on microarray slides was done at 65°C for 17 h, using Microarray Hybridization Chamber Kit (Agilent). .. Following washing steps, the microarray slides were scanned, and the data were analyzed by using Agilent Feature Extraction Software (Version 10.7) and GeneSpring GX Data Analysis Software (Version 12.0).

    Article Title: A microRNA profile in Fmr1 knockout mice reveals microRNA expression alterations with possible roles in fragile X syndrome
    Article Snippet: MicroRNA microarrays were carried out using miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (5190-0456) by Agilent Technologies ( http://www.agilent.com ). .. Oligonucleotide arrays were printed with trimer oligonucleotide probes (antisense to miRNAs) specific for 1080 mouse miRNAs using Agilent Microarray Hybridization Chamber Kit (G2534A), and miRNA expression profiling was performed and analyzed as previously described ( ; ). .. Briefly, total RNAs were isolated from the hippocampus of 3 Fmr1 KO mice and 3 WT mice using Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions.

    Article Title: Loss of Mitochondrial Functions Associated with Azole Resistance in Candida glabrata Results in Enhanced Virulence in Mice
    Article Snippet: A total of 600 ng of Cy3-labeled cRNAs from each sample in a volume of 40 μl was fragmented and hybridized for 17 h at 65°C to each array using the Gene Expression Hybridization kit (Agilent Technologies) according to the manufacturer's instructions. .. We used a Microarray Hybridization Chamber kit (Agilent Technologies) and a gasket slide with a format of eight microarrays per slide for sample hybridization to separate each sample in specific subarrays of the 8 × 15K format. ..

    Article Title: Functional Characterization of Aspergillus nidulans ypkA, a Homologue of the Mammalian Kinase SGK
    Article Snippet: The fragmentation was interrupted by adding 55 μL of 2X GE Hybridization Buffer HI-RPM. .. Finally, 100 µL of sample was placed down onto the microarray slide, which was mounted into the Agilent™ Microarray Hybridization Chamber Kit. .. The hybridization was carried out in an oven (Agilent G2545A Hybridization Oven) set to 65°C for 17 hours.

    Expressing:

    Article Title: A microRNA profile in Fmr1 knockout mice reveals microRNA expression alterations with possible roles in fragile X syndrome
    Article Snippet: MicroRNA microarrays were carried out using miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (5190-0456) by Agilent Technologies ( http://www.agilent.com ). .. Oligonucleotide arrays were printed with trimer oligonucleotide probes (antisense to miRNAs) specific for 1080 mouse miRNAs using Agilent Microarray Hybridization Chamber Kit (G2534A), and miRNA expression profiling was performed and analyzed as previously described ( ; ). .. Briefly, total RNAs were isolated from the hippocampus of 3 Fmr1 KO mice and 3 WT mice using Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions.



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    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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    Image Search Results


    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Journal: BMC Cancer

    Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

    doi: 10.1186/1471-2407-13-364

    Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing