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micro-pillar array column  (Thermo Fisher)


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    Structured Review

    Thermo Fisher micro-pillar array column
    Micro Pillar Array Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro-pillar+array+column/micropillar+array+columns/pm40305680-85-2-35
    Average 90 stars, based on 1 article reviews
    micro-pillar array column - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Ovarian SUMO-2/3 targets and their differential response to genotoxic stress induced by 7,12-dimethylbenz(a)anthracene exposure in lean and obese female mice.
    Article Snippet: SUMOylation is a post-translational modification critical for oocyte development and chromatinassociated processes.. Environmental exposures and obesity can cause follicle depletion and exposure to 7,12-dimethylbenz(a)anthracene (DMBA) altered ovarian SUMO protein abundance in lean and obese mice.. Thus, the hypothesis that exposure to DMBA may alter ovarian protein SUMOylation as a mode of ovotoxicity dampened by obesity was tested.

    Article Title: The Cyclimids: Degron-inspired cereblon binders for targeted protein degradation
    Article Snippet: The peptides were first trapped on a trapping cartridge (300 μm x 5 mm PepMap TM Neo C18 Trap Cartridge, Thermo) prior to separation on a silica-chip-based micropillar column (μPAC, C18 pillar surface, 50 cm bed, Thermo).

    Article Title: Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis
    Article Snippet: Peptide separation was performed on a 110 cm micro-pillar array column (μ-PAC Neo, Thermo Scientific) with a flow rate of ∼300 nL min −1 over a 120 min reverse phase active gradient (80% ACN in 0.1% FA from 8% to 28% over 112 min, from 28% to 48% in 8 min) followed by a column/trap wash at 80% ACN for 10 min. Eluted peptides were analyzed using a Thermo Scientific Orbitrap Exploris 480 mass spectrometer, directly coupled to the UHPLC through an Easy Spray Ion source (Thermo Scientific).

    Article Title: Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.
    Article Snippet: Peptide separation was performed on a 110 cm micro-pillar array column (μ-PAC Neo, Thermo Scientific) with a flow rate of ∼300 nL min−1 over a 120 min reverse phase active gradient (80% ACN in 0.1% FA from 8% to 28% over 112 min, from 28% to 48% in 8 min) followed by a column/trap wash at 80% ACN for 10 min. Eluted peptides were analyzed using a Thermo Scientific Orbitrap Exploris 480 mass spectrometer, directly coupled to the UHPLC through an Easy Spray Ion source (Thermo Scientific).

    Article Title: MS-Based Proteomics of Body Fluids: The End of the Beginning
    Article Snippet: Micropillar-structured columns (μPAC, Thermo Fisher) may become an attractive alternative to packed columns because of their high resolution and the absence of bead material that may change over time ( https://www.thermofisher.com/document-connect/document-connect.html?url=https://assets.thermofisher.com/TFS-Assets%2FCMD%2FTechnical-Notes%2Ftn-000635-ccs-upac-column-robustness-proteomics-tn0006358-en.pdf ) ( ).

    Activity Assay:

    Article Title: Column-Only Band Broadening in a Porous Shell Radially Elongated Pillar Array Column.
    Article Snippet: In the quest for better performing separation media for liquid chromatography, micropillar array columns have received great interest over the past years.. While previous research was mainly focused around micropillar array columns (μPACs) filled with cylindrical pillars, this contribution discusses μPACs with rectangular pillars, which, for the first time, have been anodized and hence carry a mesoporous shell.. We report on a series of on-chip measurements of the band broadening and flow permeability in a μPAC with very wide radially elongated pillars (3·75 μm) and with an interpillar distance (2 μm) between that of the first (2.5 μm) and second generation (1.25 μm) of cylindrical μPACs.

    Article Title: On-Chip Comparison of the Performance of First- and Second-Generation Micropillar Array Columns.
    Article Snippet: Because of its dimensions, the recently introduced micropillar array columns are most suited for high-efficiency liquid chromatography separations in proteomics.. Unlike the packed bed columns and capillary-based column formats, the micropillar array concept still has significant room to progress in terms of the reduction of its characteristic size (i.e., pillar diameter and interpillar distance) to open the road to even higher-efficiency separations and their applications.. We report here on the on-chip comparison between first-generation (Gen 1) and secondgeneration (Gen 2) micropillar array columns wherein the pillar and interpillar size have been halved.

    Solvent:

    Article Title: Integrating multiple omics levels using the human protein complexome as a framework, a multi-omics study of inborn errors of metabolism
    Article Snippet: Purified peptides were re-dissolved in 20 μl loading solvent A (0.1% TFA in water/ACN (98:2, v/v), the peptide concentration was determined on a Lunatic spectrophotometer (Unchained Labs) and 2 μg of each sample was injected for LC-MS/MS analysis on an Ultimate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) equipped with a pneu-Nimbus dual ion source (Phoenix S&T). .. Trapping was performed at 10 μl/min for 4 min in loading solvent A on a 20 mm trapping column (made in-house, 100 μm inner diameter(ID), 5 μm beads, C18 Reprosil-HD, Dr. Maisch) and the sample was loaded on a 200 cm long micro pillar array column (Thermo Scientific) with C18-endcapped functionality mounted in the Ultimate 3000’s column oven at 50°C. .. For proper ionization, a fused silica PicoTip emitter (10 μm ID, New Objective) was connected to the μPACTM outlet union and a grounded connection was provided to this union.



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