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PharmaFluidics n.v 200 cm micro-pillar array column
200 Cm Micro Pillar Array Column, supplied by PharmaFluidics n.v, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/micro-pillar+array+column/micro+pillar+array+column/pm39081016-78-8-12
Average 90 stars, based on 1 article reviews
200 cm micro-pillar array column - by Bioz Stars, 2026-09
90/100 stars

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Article Title: TurboID-based proteomic profiling reveals proxitome of ASK1 and CUL1 of the SCF ubiquitin ligase in plants.
Article Snippet: Sample separation was performed on a 200 cm Micro-Pillar Array Column (lPAC, Pharmafluidics) with a flow rate of c. 300 nl min 1 over a 150 min reverse phase gradient (80% ACN in 0.1% FA from 1% to 15% over 5 min, 15% to 20.8% over 20 min, from 20.8% to 43.8% over 80 min, and from 43.8% to 99.0% in 11 min, and kept at 99.0% for 5 min).

Article Title: Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes.
Article Snippet: Trapping was performed as described above and peptides were again separated on a 200 cm-long micropillar array column (μPAC, PharmaFluidics) with C18-endcapped functionality.

Article Title: Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial.
Article Snippet: The performance of the current bottom-up liquid chromatography hyphenated with mass spectrometry (LC-MS) analyses has undoubtedly been fueled by spectacular progress in mass spectrometry.. It is thus not surprising that the MS instrument attracts the most attention during LC-MS method development, whereas optimizing conditions for peptide separation using reversed-phase liquid chromatography (RPLC) remains somewhat in its shadow.. Consequently, the wisdom of the fundaments of chromatography is slowly vanishing from some laboratories.

Article Title: PLASMODESMATA-LOCATED PROTEIN 6 regulates plasmodesmal function in Arabidopsis vasculature
Article Snippet: Sample separation was performed on a 200 cm Micro-Pillar Array Column (μ-PAC, PharmaFluidics) with a flow rate of ∼300 nL/min over a 150 min reverse phase gradient (80% ACN in 0.1% FA from 1% to 15% over 5 min, from 15% to 20.8% over 20 min, from 20.8% to 43.8% over 80 min, and from 43.8% to 99.0% in 11 min and kept at 99.0% for 5 min).

Article Title: Novel Stenotrophomonas maltophilia Bacteriophage as Potential Therapeutic Agent
Article Snippet: Of the peptide mixture, 0.5 μg was loaded on a 200 cm micro pillar array column (mPACTM, PharmaFluidics, Ghent, Belgium) retrofitted to a NanoSpray Flex source.

Article Title: Trypanosoma cruzi reprograms mitochondrial metabolism within the anterior midgut of its vector Rhodnius prolixus during the early stages of infection
Article Snippet: Trapping was performed at 10 μl/min for 4 min in solvent A on a 20-mm trapping column (made in-house, 100 μm internal diameter, 5-μm beads, C18 ReproSil-HD, Dr. Maisch, Germany), and the sample was loaded on a 200-cm-long micro-pillar array column (PharmaFluidics, Ghent, Belgium) with C18 end-capped functionality mounted in the UltiMate 3000 column oven at 50 °C.

Solvent:

Article Title: Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins.
Article Snippet: Peptides were purified on Omix C18 tips (Agilent), dried, and redissolved in 20 μL loading solvent (0.1% trifluoroacetic acid in water/ acetonitrile (ACN) (98:2, v/v)) of which 6 μL were injected for LC-MS/ MS analysis on an Ultimate 3000 RSLCnano system (Thermo) in-line connected to a Q Exactivemass spectrometer (Thermo). .. Trapping was performed at 10μL/min for 4min in loading solvent A on a 10mm μPACTM trapping column (PharmaFluidics) with C18-endcapped stationary phaseand the sampleswere loadedon a 50 cm longmicropillar array column (PharmaFluidics) with C18-endcapped functionality mounted in the Ultimate 3000’s column oven set at 35 °C. .. For proper ionization, a fused silica PicoTip emitter (10 μm inner diameter, New Objective) was connected to the μPACTM outlet union and a grounded connection was provided to this union.

Article Title: Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins
Article Snippet: Peptides were purified on Omix C18 tips (Agilent), dried, and re-dissolved in 20 μL loading solvent (0.1% trifluoroacetic acid in water/acetonitrile (ACN) (98:2, v/v)) of which 6 μL were injected for LC-MS/MS analysis on an Ultimate 3000 RSLCnano system (Thermo) in-line connected to a Q Exactive mass spectrometer (Thermo). .. Trapping was performed at 10 μL/min for 4 min in loading solvent A on a 10 mm μPAC TM trapping column (PharmaFluidics) with C18-endcapped stationary phase and the samples were loaded on a 50 cm long micropillar array column (PharmaFluidics) with C18-endcapped functionality mounted in the Ultimate 3000’s column oven set at 35 °C. .. For proper ionization, a fused silica PicoTip emitter (10 μm inner diameter, New Objective) was connected to the μPACTM outlet union and a grounded connection was provided to this union.



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