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tracking module  (MetaMorph Inc)

 
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    MetaMorph Inc tracking module
    Tracking Module, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/metamorph+tracking/tracking+module/us09698708-137-8-8
    Average 90 stars, based on 1 article reviews
    tracking module - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Glutamine Uptake via SNAT6 and Caveolin Regulates Glutamine–Glutamate Cycle
    Article Snippet: The internalization here was monitored in real-time by tracking the caveolin rich sites using a Metamorph tracking plugin ( J, zoomed tracks in the inset).

    Article Title: A microfluidic system to study cytoadhesion of Plasmodium falciparum infected erythrocytes to primary brain microvascularendothelial cells
    Article Snippet: The design of the μF-CS was optimized to provide a stable environment to quantify cytoadhesion of parasitized erythrocytes to endothelial cells.Uninfected erythrocytes were not observed to roll on the endothelial cells but rather occasionally bind and then release without rolling.The tracking methods offered in MetaMorph failed to reliably track rolling parasites, particularly when the parasite was obfuscated either by the endothelial cells or in some cases by a high concentration of erythrocytes.

    Migration:

    Article Title: Cadherin-11 inhibitors and methods of use thereof
    Article Snippet: Phase images were recorded immediately following scraping and every hour using 10× objective (Nikon Eclipse TE-300 inverted microscope with motorized stage and CO2-regulated chamber, controlled by the Multidimensional Analysis tool of Metamorph Image Acquisitions software; Tokyo, JP). .. Migration distances were manually calculated by caliper scaling of wound width using Metamorph tracking module. .. Matrigel Outgrowth Assays: Cells (5,000 cells/100 μl medium) were plated atop 150 μL layer of Matrigel (BD Biosciences; San Jose, Calif.) in duplicate in 12-well glass-bottom dishes (MatTek; Ashland, Mass.) and incubated for 30 minutes at 37° C. Growth medium (1 mL) was gently added to each well.

    Article Title: P-cadherin is a direct PAX3-FOXO1A target involved in alveolar rhabdomyosarcoma aggressiveness.
    Article Snippet: Phase-contrast images were taken every 20–30 min overnight using a 10/0.3 NA PH1 DIC1 objective and captured with a CCD MicroMax 1300 Y/HS camera (Roper Scientific, Evry, France) controlled by the Metamorph 7.0 software. .. The migration speeds were calculated by tracking the nucleus of individual cells using the tracking module of Metamorph. ..

    Software:

    Article Title: Polymer-based tubular microbots: role of composition and preparation.
    Article Snippet: An inverted optical microscope (Nikon Instrument Inc. TiS/L100), coupled with a 40x objective, a Photometrics QuantEM 512/SC camera (Roper Scientific, Duluth, GA) and MetaMorph 7.6 software (Molecular Devices, Sunnyvale, CA) were used for capturing movies at a frame rate of 30 frames per sec. .. The speeds of the microbots were tracked using a Metamorph tracking module and the results were statistically analyzed using Origin software. .. In order to self-propel catalytic microbots, aqueous hydrogen peroxide solutions (Sigma-Aldrich, cat. 95313) with concentrations ranging from 0.5–15.0% were used as chemical fuels, containing 2–5.0% (w/v) sodium cholate (Sigma-Aldrich, St Louis, MO) to reduce the surface tension, hence facilitating the microbot propulsion.



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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    particle tracking metamorph  (MetaMorph Inc)
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    Image Search Results


    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ plugin (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from Metamorph tracking (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.

    Journal: International Journal of Molecular Sciences

    Article Title: Glutamine Uptake via SNAT6 and Caveolin Regulates Glutamine–Glutamate Cycle

    doi: 10.3390/ijms22031167

    Figure Lengend Snippet: SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ plugin (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from Metamorph tracking (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.

    Article Snippet: Tracking experiments were performed using a Metamorph tracking plugin with pixel size cut off = 5.

    Techniques: Expressing, Microscopy, Fluorescence, Labeling, Derivative Assay, Transfection, Sequencing