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particle tracking metamorph  (MetaMorph Inc)

 
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    MetaMorph Inc particle tracking metamorph
    Particle Tracking Metamorph, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/metamorph+tracking/particle+tracking+algorithm+trackobj/pmc06018151-339-0-2
    Average 90 stars, based on 1 article reviews
    particle tracking metamorph - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion.
    Article Snippet: Collected experimental data were processed by particle tracking algorithms of the Metamorph software.

    Article Title: SHIP-1 Increases Early Oxidative Burst and Regulates Phagosome Maturation in Macrophages
    Article Snippet: Recruitment of YFP chimeras to phagosomes was measured using the particle-tracking algorithm TRACKOBJ in Metamorph.

    Article Title: SHIP-1 Increases Early Oxidative Burst and Regulates Phagosome Maturation in Macrophages
    Article Snippet: Particle Tracking Recruitment of YFP chimeras to phagosomes was measured using the particle-tracking algorithm TRACKOBJ in Metamorph.

    Article Title: Sequential signaling in plasma-membrane domains during macropinosome formation in macrophages
    Article Snippet: Signaling from multiple cellular events was measured by a particle-tracking image-analysis algorithm, developed using MetaMorph software, which tracked organelles or regions of an image in the phase-contrast image series by a cross-correlation centroid-tracking algorithm `TRACOBJ' ( Henry et al., 2004 ; Hoppe et al., 2004; Beemiller et al., 2006 ).

    Article Title: Spontaneous buckling of contractile poroelastic actomyosin sheets
    Article Snippet: Particle tracking (Metamorph and Matlab) was used to extract the center-of-mass position of the beads as a function of time.

    Article Title: Nanoconfined Atomic Layer Deposition of TiO 2 /Pt Nanotubes: Toward Ultrasmall Highly Efficient Catalytic Nanorockets
    Article Snippet: Particle tracking was subsequently performed in Metamorph to capture the average nanorocket speed for each nanorocket sample.

    Article Title: A Cdc42 Activation Cycle Coordinated by PI 3-Kinase during Fc Receptor-mediated Phagocytosis
    Article Snippet: To measure FRET or R i as a function of time during phagocytosis, the TRACKOBJ particle-tracking algorithm of Metamorph was used to identify and track erythrocytes or microspheres bound by macrophages and to automate the measurement of average gray values in a predefined measurement circle centered around the target particle, as described previously ( Henry et al. , 2004 ; ).

    Article Title: Role of SpdA in Cell Spreading and Phagocytosis in Dictyostelium
    Article Snippet: We used Particle tracking from the Metamorph software to track individual cell trajectories.



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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
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    https://www.bioz.com/product/metamorph+tracking/multi+dimensional++single+particle+tracking+and+motion+analysis+modules+of+metamorph+v++7+7+2+0/pmc06302550-466-18-17
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    particle tracking metamorph  (MetaMorph Inc)
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
    Particle Tracking Metamorph, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/metamorph+tracking/particle+tracking+algorithm+trackobj/pmc06018151-339-0-2
    Average 90 stars, based on 1 article reviews
    particle tracking metamorph - by Bioz Stars, 2026-09
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    		  Buy from Supplier
    tracking module metamorph  (MetaMorph Inc)
    90
    MetaMorph Inc tracking module metamorph
    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
    Tracking Module Metamorph, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/metamorph+tracking/metamorph+tracking+module/us09868991-580-6-6
    Average 90 stars, based on 1 article reviews
    tracking module metamorph - by Bioz Stars, 2026-09
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    SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ plugin (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from Metamorph tracking (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.

    Journal: International Journal of Molecular Sciences

    Article Title: Glutamine Uptake via SNAT6 and Caveolin Regulates Glutamine–Glutamate Cycle

    doi: 10.3390/ijms22031167

    Figure Lengend Snippet: SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ plugin (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from Metamorph tracking (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.

    Article Snippet: Tracking experiments were performed using a Metamorph tracking plugin with pixel size cut off = 5.

    Techniques: Expressing, Microscopy, Fluorescence, Labeling, Derivative Assay, Transfection, Sequencing