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MetaMorph Inc track points function in
Track Points Function In, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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track points function in - by Bioz Stars, 2026-09
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Related Articles

Migration:

Article Title: Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration.
Article Snippet: Time-lapse images were obtained at 15-min intervals over 12 h using a Rolera EM-C2 EMCCD camera operated by Zen image analysis software (Zeiss). .. To calculate cell migration parameters, the centers of cell nuclei of cells at wound edge were manually tracked and positions recorded from the timelapse image series using the “track points” function in Metamorph. ..

Article Title: Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration
Article Snippet: Time-lapse images were obtained at 15-min intervals over 12 h using a Rolera EM-C 2 EMCCD camera operated by Zen image analysis software (Zeiss). .. To calculate cell migration parameters, the centers of cell nuclei of cells at wound edge were manually tracked and positions recorded from the time-lapse image series using the “track points” function in Metamorph. ..

Article Title: ERK reinforces actin polymerization to power persistent edge protrusion during motility
Article Snippet: .. For migration assay, displacement or the distance migrated over a 6 hour period was calculated using the Track Points function in Metamorph. ..

other:

Article Title: MARK2 regulates directed cell migration through modulation of myosin II contractility and focal adhesion organization.
Article Snippet: COS1 cells in a 10 cm dish were transiently transfected for 24-36 h with 5 mg of DNA vector using XtremeGene 9 (Roche) transfection reagent.

Microscopy:

Article Title: Transient lamellipodia predict sites of dendritic branch formation in hippocampal neurons.
Article Snippet: Extensive branching creates the complex dendritic arbor of mammalian CNS neurons but capturing the complete process of branch formation with time-lapse recordings has been challenging.. Here, we report that application of BMP7 to cultured hippocampal neurons accelerated dendritic growth sufficiently to document branches forming in less than 20 h via frequent time-lapse imaging (10-min intervals).. In these recordings, most branches emerged as collateral sprouts from the shaft of a parent branch.

Control:

Article Title: Transient lamellipodia predict sites of dendritic branch formation in hippocampal neurons.
Article Snippet: Extensive branching creates the complex dendritic arbor of mammalian CNS neurons but capturing the complete process of branch formation with time-lapse recordings has been challenging.. Here, we report that application of BMP7 to cultured hippocampal neurons accelerated dendritic growth sufficiently to document branches forming in less than 20 h via frequent time-lapse imaging (10-min intervals).. In these recordings, most branches emerged as collateral sprouts from the shaft of a parent branch.



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MetaMorph Inc metamorph tracking plugin
SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Metamorph Tracking Plugin, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/metamorph+tracking+module/pmc07865731-286-7-6
Average 90 stars, based on 1 article reviews
metamorph tracking plugin - by Bioz Stars, 2026-09
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SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Metamorph Track Points, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Track Points For Metamorph Analysis Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/tracking+object+mode+of+metamorph+software/pmc07455044__aba7910_SM-71-12-15
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SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Tracking Tool In Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Particle Tracking Function In Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Track Object Of Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/tracking+object+mode+of+metamorph+software/pmc06820738-242-12-15
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SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Single Particle Tracking With The Metamorph Track Objects Plugin, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Particle Tracking Metamorph, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/particle+tracking+algorithm+trackobj/pmc06018151-339-0-2
Average 90 stars, based on 1 article reviews
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SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ <t>plugin</t> (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from <t>Metamorph</t> <t>tracking</t> (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.
Tracking Module Metamorph, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/metamorph+tracking/metamorph+tracking+module/us09868991-580-6-6
Average 90 stars, based on 1 article reviews
tracking module metamorph - by Bioz Stars, 2026-09
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Image Search Results


SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ plugin (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from Metamorph tracking (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.

Journal: International Journal of Molecular Sciences

Article Title: Glutamine Uptake via SNAT6 and Caveolin Regulates Glutamine–Glutamate Cycle

doi: 10.3390/ijms22031167

Figure Lengend Snippet: SNAT6 associated caveolin complexes internalize in response to glutamine and glutamate in a Na + dependent manner. ( A ) Images of cells expressing SNAT6-EGFP and Caveolin1-mCherry under TIRF microscopy before and after stimulation with glutamine. An ImageJ plugin (described in methods) detecting the SNAT6 and caveolin clusters. ( B ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified using the method above before and after stimulations with different amino acids specified in the figure (density of clusters was significantly decreased under Glu, Gln, and Ala, p < 0.001). ( C ) Trace showing fluorescence of the entire single cell for caveolin. Note that the stimulation with glutamine was initiated at time zero. ( D ) Images from antibody labeled SNAT6 and caveolin localizing together (44 ± 7% in six cells derived from two independent experiments). ( E ) Similar to C but showing average fluorescence for 10 cells from three different transfections under glutamate stimulation. ( F – I ) Similar to E for cells stimulated with glutamine (11 cells), alanine (13 cells), lysine (12 cells), and proline (9 cells). All the experiments were performed from at least three different transfections. ( J ) Images showing caveolin-mCherry expressing cells with and without Na + present in the buffer when stimulated with glutamine. Tracks obtained from Metamorph tracking (described in methods) during the entire time sequence. zoomed tracks and images in the inset ( K ) Average speed for the tracks in ( J ) in presence and absence of Na + in the buffer under glutamine stimulation (* p < 0.05). ( L ) Histogram of 16–60 tracks from 9 different cells per condition similar to the cell shown in ( I ). Displacement of caveolin in presence (lavender) and absence (orange) of Na + in the buffer under glutamine stimulation. ( M ) Density of SNAT6 (green) and Caveolin (grey) clusters quantified as in B before and after stimulations with glutamine in presence and absence of Na + in the buffer.

Article Snippet: Tracking experiments were performed using a Metamorph tracking plugin with pixel size cut off = 5.

Techniques: Expressing, Microscopy, Fluorescence, Labeling, Derivative Assay, Transfection, Sequencing