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Mega6, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mega6+program/mega6/pm26950513-97-36-13
Average 90 stars, based on 1 article reviews
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Construct:Article Title: Dual nuclease activity of a Cas2 protein in CRISPR-Cas subtype I-B of Leptospira interrogans. Article Snippet: Edited by Renee Tsolis Leptospira interrogans serovar Copenhageni strain Fiocruz L1–130 carries a set of cas genes associated with CRISPR–Cas subtype I-B.. Herein, we report for the first time active transcription of a set of cas genes (cas1 to cas8) of L. interrogans where cas4, cas1, cas2 and cas6, cas3, cas8, cas7, cas5 are clustered together in two independent operons.. As an initial step toward comprehensive understanding of CRISPR–Cas system in spirochete, the biochemical study of one of the core Leptospira Cas2 proteins (Lep_Cas2) showed nuclease activity on both DNA and RNA in a nonspecific manner. |
