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sh30521 02  (Danaher Inc)


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    Structured Review

    Danaher Inc sh30521 02
    Sh30521 02, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/medium/HyClone+SFM4HEK293+liquid+medium+with+L-glutamine/pmc07762703-17-5-3
    Average 96 stars, based on 12 article reviews
    sh30521 02 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Process/Product Development:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Cell Culture:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Suspension:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Transfection:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Plasmid Preparation:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Centrifugation:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Transferring:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Expressing:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Microscopy:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Electron Microscopy:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.

    Recombinant:

    Article Title: Backbone-Minimised Nanoplasmid DNA Systems Enable High-Titre AAV Production in Suspension HEK293 Platforms
    Article Snippet: t workflows for gene therapy manufacturing. Suspension HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA, catalogue no. R78005) were maintained in HyClone™ SFM4HEK293 (Peak) serum-free medium (Cytiva, Wilmington, DE, USA, catalogue no. SH30521.02) in 125 mL polycarbonate Erlenmeyer flasks (Corning, New York, NY, USA, catalogue no. 431143) at a working volume of 30 mL. Cells were cultured at

    Article Title: Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
    Article Snippet: ExpiFectamine 293 Transfection Kit , GIBCO , A14524.ExpiFectamine 293 Transfection Kit , GIBCO , A14524.. Hyclone SFM4HEK293 , Cytiva , SH30521.02.. Opti-MEM I , GIBCO , 31,985-070.Opti-MEM I , GIBCO , 31,985-070.



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    Image Search Results


    In vitro evaluation of the osteogenic-promoting capability of BTO@ALN. (A–D) Immunofluorescence staining of osteoblasts differentiated from primary mouse bone marrow–derived mesenchymal stem cells (BMSCs) (red: actin; green: RUNX2 and ATF4) and corresponding quantitative analysis of fluorescence intensity. (E–H) Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of osteoblasts derived from primary mouse BMSCs and quantitative analysis of staining intensity.

    Journal: Bioactive Materials

    Article Title: A motion-activated skeletal “Pacemaker” for systemic bone remodeling via oral piezoelectric microspheres

    doi: 10.1016/j.bioactmat.2026.05.051

    Figure Lengend Snippet: In vitro evaluation of the osteogenic-promoting capability of BTO@ALN. (A–D) Immunofluorescence staining of osteoblasts differentiated from primary mouse bone marrow–derived mesenchymal stem cells (BMSCs) (red: actin; green: RUNX2 and ATF4) and corresponding quantitative analysis of fluorescence intensity. (E–H) Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of osteoblasts derived from primary mouse BMSCs and quantitative analysis of staining intensity.

    Article Snippet: Osteogenic differentiation was induced by culturing BMSCs in osteogenic induction medium (Procell, Wuhan, China).

    Techniques: In Vitro, Immunofluorescence, Staining, Derivative Assay, Fluorescence