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texmacs medium  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec texmacs medium
    Texmacs Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 598 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/medium/TexMACS+Medium/pm41691247-91-5-7
    Average 97 stars, based on 598 article reviews
    texmacs medium - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Combination of compositions for elimination and enhanced engraftment of hematopoietic stem cells in the bone marrow of a subject
    Article Snippet: For T cell activation and -expansion the T cells were seeded at 1×106 cells/mL in TexMACS medium containing IL-7 (10 ng/mL) and IL-15 (10 ng/mL) and 1% (v/v) T Cell TransAct, human (Miltenyi Biotec, Art No: 130-111-160) and incubated at 37° C., 5% CO2. .. For T cell activation and -expansion the T cells were seeded at 1×106 cells/mL in TexMACS medium containing IL-7 (10 ng/mL) and IL-15 (10 ng/mL) and 1% (v/v) T Cell TransAct, human (Miltenyi Biotec, Art No: 130-111-160) and incubated at 37° C., 5% CO2. ..

    Incubation:

    Article Title: Combination of compositions for elimination and enhanced engraftment of hematopoietic stem cells in the bone marrow of a subject
    Article Snippet: For T cell activation and -expansion the T cells were seeded at 1×106 cells/mL in TexMACS medium containing IL-7 (10 ng/mL) and IL-15 (10 ng/mL) and 1% (v/v) T Cell TransAct, human (Miltenyi Biotec, Art No: 130-111-160) and incubated at 37° C., 5% CO2. .. For T cell activation and -expansion the T cells were seeded at 1×106 cells/mL in TexMACS medium containing IL-7 (10 ng/mL) and IL-15 (10 ng/mL) and 1% (v/v) T Cell TransAct, human (Miltenyi Biotec, Art No: 130-111-160) and incubated at 37° C., 5% CO2. ..

    Isolation:

    Article Title: Bidirectional modulation of aging-associated cellular phenotypes by mitochondrial genome replacement
    Article Snippet: CD3+ or CD8+ T cells were isolated from mouse spleens by negative selection using the EasySepTM Mouse T Cell Isolation Kit (STEMCELL Technologies, 19851) or EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853), respectively. .. Isolated T cells were cultured in RPMI 1640 medium containing L-glutamine (Nacalai Tesque, 30264-85) or TexMACS medium (Miltenyi Biotec, 130-097-196) at 37°C under 5% CO2. .. Normal human dermal fibroblasts (NHDFs) were obtained from Lonza (CC-2511) or PromoCell (C-12302).

    Article Title: Method for transduction of t cells in the presence of malignant cells
    Article Snippet: .. Primary T cells of 2 donors were isolated from PBMC using the PAN T cell isolation Kit (Miltenyi Biotec, 130-096-535) and cultivated in TexMACS medium (Miltenyi Biotec, 130-097-196) with IL7 (Miltenyi Biotec, 170-076-111) IL15 (Miltenyi Biotec, 170-076-114) and Transact (Miltenyi Biotec, 130-111-160) over night at 37° C. Half of the samples were treated with the integration inhibitor Raltegravir (Sigma Aldrich, cat. No. CDS023737-25MG). ..

    Cell Culture:

    Article Title: Bidirectional modulation of aging-associated cellular phenotypes by mitochondrial genome replacement
    Article Snippet: CD3+ or CD8+ T cells were isolated from mouse spleens by negative selection using the EasySepTM Mouse T Cell Isolation Kit (STEMCELL Technologies, 19851) or EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853), respectively. .. Isolated T cells were cultured in RPMI 1640 medium containing L-glutamine (Nacalai Tesque, 30264-85) or TexMACS medium (Miltenyi Biotec, 130-097-196) at 37°C under 5% CO2. .. Normal human dermal fibroblasts (NHDFs) were obtained from Lonza (CC-2511) or PromoCell (C-12302).

    Article Title: CAR T cells secreting anti-EpCAM bi-specific T cell engagers overcome tumor heterogeneity in targeting various epithelial originated carcinomas.
    Article Snippet: To introduce the 682 CAR genes, the activated T cells were mixed with viral supernatant in the presence of polybrene 683 (8 μg/mL; Sigma Aldrich) and transferred to 6-well plates for spin infection at 1,200 × g, 30°C for 684 90 minutes. .. Transduced cells were pooled and cultured in fresh TexMACSTM medium (Miltenyi 685 Biotech, catalog no. 130-097-196) in the presence of either human IL-2 (50 unit per ml) or human 686 IL-7 (20 ng/ml, Miltenyi Biotech, catalog no. 130-095-363) plus human IL-15 (5 ng/ml, Miltenyi 687 Biotech, catalog no. 130-095-765) for 9-14 days. ..

    Article Title: Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening.
    Article Snippet: .. T cells were purified using the EasySep Human T cell Isolation Kit (STEMCELL Technologies 17951) and cultured in TexMACS medium (Miltenyi Biotec 130-097-196) supplemented with 5% Fetalgro bovine growth serum and 200 IU/mL recombinant human IL-2 (Miltenyi Biotec 130-097-743). .. T cells were activated with T cell TransAct beads (Miltenyi Biotec 130-128-758) one day prior to transduction.

    Purification:

    Article Title: Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening.
    Article Snippet: .. T cells were purified using the EasySep Human T cell Isolation Kit (STEMCELL Technologies 17951) and cultured in TexMACS medium (Miltenyi Biotec 130-097-196) supplemented with 5% Fetalgro bovine growth serum and 200 IU/mL recombinant human IL-2 (Miltenyi Biotec 130-097-743). .. T cells were activated with T cell TransAct beads (Miltenyi Biotec 130-128-758) one day prior to transduction.

    Cell Isolation:

    Article Title: Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening.
    Article Snippet: .. T cells were purified using the EasySep Human T cell Isolation Kit (STEMCELL Technologies 17951) and cultured in TexMACS medium (Miltenyi Biotec 130-097-196) supplemented with 5% Fetalgro bovine growth serum and 200 IU/mL recombinant human IL-2 (Miltenyi Biotec 130-097-743). .. T cells were activated with T cell TransAct beads (Miltenyi Biotec 130-128-758) one day prior to transduction.

    Article Title: Method for transduction of t cells in the presence of malignant cells
    Article Snippet: .. Primary T cells of 2 donors were isolated from PBMC using the PAN T cell isolation Kit (Miltenyi Biotec, 130-096-535) and cultivated in TexMACS medium (Miltenyi Biotec, 130-097-196) with IL7 (Miltenyi Biotec, 170-076-111) IL15 (Miltenyi Biotec, 170-076-114) and Transact (Miltenyi Biotec, 130-111-160) over night at 37° C. Half of the samples were treated with the integration inhibitor Raltegravir (Sigma Aldrich, cat. No. CDS023737-25MG). ..

    Recombinant:

    Article Title: Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening.
    Article Snippet: .. T cells were purified using the EasySep Human T cell Isolation Kit (STEMCELL Technologies 17951) and cultured in TexMACS medium (Miltenyi Biotec 130-097-196) supplemented with 5% Fetalgro bovine growth serum and 200 IU/mL recombinant human IL-2 (Miltenyi Biotec 130-097-743). .. T cells were activated with T cell TransAct beads (Miltenyi Biotec 130-128-758) one day prior to transduction.

    Article Title: Bioluminescent xenograft model of acute myeloid leukemia for immunotherapy preclinical studies.
    Article Snippet: Accurate in vivo models that recapitulate both acute myeloid leukemia (AML) progression and human immune responses are essential for the preclinical testing of novel immunotherapies.. In this study, we developed and validated a bioluminescent NSG-SGM3 PBMC-THP-1 xenograft model that does not require cytotoxic or irradiation preconditioning and allows simultaneous assessment of leukemia burden, human chimerism, and immune checkpoint expression.. Based on six consecutive experiments, we established that GFP/Luc-THP-1 is the preferred clone for the NSG-SGM3 strain when co-transplanted with healthy donor peripheral blood mononuclear cells (PBMC).



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    Image Search Results


    In vitro evaluation of the osteogenic-promoting capability of BTO@ALN. (A–D) Immunofluorescence staining of osteoblasts differentiated from primary mouse bone marrow–derived mesenchymal stem cells (BMSCs) (red: actin; green: RUNX2 and ATF4) and corresponding quantitative analysis of fluorescence intensity. (E–H) Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of osteoblasts derived from primary mouse BMSCs and quantitative analysis of staining intensity.

    Journal: Bioactive Materials

    Article Title: A motion-activated skeletal “Pacemaker” for systemic bone remodeling via oral piezoelectric microspheres

    doi: 10.1016/j.bioactmat.2026.05.051

    Figure Lengend Snippet: In vitro evaluation of the osteogenic-promoting capability of BTO@ALN. (A–D) Immunofluorescence staining of osteoblasts differentiated from primary mouse bone marrow–derived mesenchymal stem cells (BMSCs) (red: actin; green: RUNX2 and ATF4) and corresponding quantitative analysis of fluorescence intensity. (E–H) Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of osteoblasts derived from primary mouse BMSCs and quantitative analysis of staining intensity.

    Article Snippet: Osteogenic differentiation was induced by culturing BMSCs in osteogenic induction medium (Procell, Wuhan, China).

    Techniques: In Vitro, Immunofluorescence, Staining, Derivative Assay, Fluorescence