texmacs medium (Miltenyi Biotec)
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Texmacs Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 598 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/medium/TexMACS+Medium/pm41691247-91-5-7
Average 97 stars, based on 598 article reviews
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Activation Assay:Article Title: Combination of compositions for elimination and enhanced engraftment of hematopoietic stem cells in the bone marrow of a subject Article Snippet: For T cell activation and -expansion the T cells were seeded at 1×106 cells/mL in TexMACS medium containing IL-7 (10 ng/mL) and IL-15 (10 ng/mL) and 1% (v/v) T Cell TransAct, human (Miltenyi Biotec, Art No: 130-111-160) and incubated at 37° C., 5% CO2. .. For T cell activation and -expansion the T cells were seeded at 1×106 cells/mL in Incubation:Article Title: Combination of compositions for elimination and enhanced engraftment of hematopoietic stem cells in the bone marrow of a subject Article Snippet: For T cell activation and -expansion the T cells were seeded at 1×106 cells/mL in TexMACS medium containing IL-7 (10 ng/mL) and IL-15 (10 ng/mL) and 1% (v/v) T Cell TransAct, human (Miltenyi Biotec, Art No: 130-111-160) and incubated at 37° C., 5% CO2. .. For T cell activation and -expansion the T cells were seeded at 1×106 cells/mL in Isolation:Article Title: Bidirectional modulation of aging-associated cellular phenotypes by mitochondrial genome replacement Article Snippet: CD3+ or CD8+ T cells were isolated from mouse spleens by negative selection using the EasySepTM Mouse T Cell Isolation Kit (STEMCELL Technologies, 19851) or EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853), respectively. .. Isolated T cells were cultured in RPMI 1640 medium containing L-glutamine (Nacalai Tesque, 30264-85) or Article Title: Method for transduction of t cells in the presence of malignant cells Article Snippet: .. Primary T cells of 2 donors were isolated from PBMC using the PAN T cell isolation Kit (Miltenyi Biotec, 130-096-535) and cultivated in Cell Culture:Article Title: Bidirectional modulation of aging-associated cellular phenotypes by mitochondrial genome replacement Article Snippet: CD3+ or CD8+ T cells were isolated from mouse spleens by negative selection using the EasySepTM Mouse T Cell Isolation Kit (STEMCELL Technologies, 19851) or EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19853), respectively. .. Isolated T cells were cultured in RPMI 1640 medium containing L-glutamine (Nacalai Tesque, 30264-85) or Article Title: CAR T cells secreting anti-EpCAM bi-specific T cell engagers overcome tumor heterogeneity in targeting various epithelial originated carcinomas. Article Snippet: To introduce the 682 CAR genes, the activated T cells were mixed with viral supernatant in the presence of polybrene 683 (8 μg/mL; Sigma Aldrich) and transferred to 6-well plates for spin infection at 1,200 × g, 30°C for 684 90 minutes. .. Transduced cells were pooled and cultured in fresh Article Title: Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening. Article Snippet: .. T cells were purified using the EasySep Human T cell Isolation Kit (STEMCELL Technologies 17951) and cultured in Purification:Article Title: Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening. Article Snippet: .. T cells were purified using the EasySep Human T cell Isolation Kit (STEMCELL Technologies 17951) and cultured in Cell Isolation:Article Title: Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening. Article Snippet: .. T cells were purified using the EasySep Human T cell Isolation Kit (STEMCELL Technologies 17951) and cultured in Article Title: Method for transduction of t cells in the presence of malignant cells Article Snippet: .. Primary T cells of 2 donors were isolated from PBMC using the PAN T cell isolation Kit (Miltenyi Biotec, 130-096-535) and cultivated in Recombinant:Article Title: Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening. Article Snippet: .. T cells were purified using the EasySep Human T cell Isolation Kit (STEMCELL Technologies 17951) and cultured in Article Title: Bioluminescent xenograft model of acute myeloid leukemia for immunotherapy preclinical studies. Article Snippet: Accurate in vivo models that recapitulate both acute myeloid leukemia (AML) progression and human immune responses are essential for the preclinical testing of novel immunotherapies.. In this study, we developed and validated a bioluminescent NSG-SGM3 PBMC-THP-1 xenograft model that does not require cytotoxic or irradiation preconditioning and allows simultaneous assessment of leukemia burden, human chimerism, and immune checkpoint expression.. Based on six consecutive experiments, we established that GFP/Luc-THP-1 is the preferred clone for the NSG-SGM3 strain when co-transplanted with healthy donor peripheral blood mononuclear cells (PBMC). |
