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axiovision colocalization module  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axiovision colocalization module
    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the <t>colocalization.</t> B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.
    Axiovision Colocalization Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/measure+colocalization+module/AxioVision+4+Module+Interactive+Measurement/pmc03315488-284-8-11
    Average 96 stars, based on 1 article reviews
    axiovision colocalization module - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice"

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1002575

    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.
    Figure Legend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Techniques Used: Injection, Immunohistofluorescence, Expressing, Infection

    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.
    Figure Legend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Techniques Used: Injection, Immunohistofluorescence, Immunofluorescence, Expressing

    Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.
    Figure Legend Snippet: Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Techniques Used: Immunohistofluorescence, Expressing, Infection, Injection

    Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.
    Figure Legend Snippet: Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Techniques Used: Injection, Immunohistofluorescence, Expressing, Infection

    Related Articles

    Control:

    Article Title: Keratin-72 restricts HIV-1 infection in resting CD4 + T cells by sequestering capsids in intermediate filaments.
    Article Snippet: HeLa cells, MDMs, or MDDCs were fixed and stained primarily with anti-p24 that was probedwith an Alexa Fluor 488 conjugated goat anti-rabbit or anti-mouse secondary antibody (Imag-iT Fixation/Permeabilization Kit, Invitrogen) according to the manufacturer’s instructions. .. The colocalization coefficient of mCherry-KRT72 or control signals with HIV-1 core signals from INmNG (VSV-G-pseudotyped HIV-1) or anti-p24 antibody (WT HIV-1) staining was calculated by the colocalization module of Carl Zeiss Micro-imaging Zen software Blue version 3.3. ..

    Staining:

    Article Title: Keratin-72 restricts HIV-1 infection in resting CD4 + T cells by sequestering capsids in intermediate filaments.
    Article Snippet: HeLa cells, MDMs, or MDDCs were fixed and stained primarily with anti-p24 that was probedwith an Alexa Fluor 488 conjugated goat anti-rabbit or anti-mouse secondary antibody (Imag-iT Fixation/Permeabilization Kit, Invitrogen) according to the manufacturer’s instructions. .. The colocalization coefficient of mCherry-KRT72 or control signals with HIV-1 core signals from INmNG (VSV-G-pseudotyped HIV-1) or anti-p24 antibody (WT HIV-1) staining was calculated by the colocalization module of Carl Zeiss Micro-imaging Zen software Blue version 3.3. ..

    Software:

    Article Title: Keratin-72 restricts HIV-1 infection in resting CD4 + T cells by sequestering capsids in intermediate filaments.
    Article Snippet: HeLa cells, MDMs, or MDDCs were fixed and stained primarily with anti-p24 that was probedwith an Alexa Fluor 488 conjugated goat anti-rabbit or anti-mouse secondary antibody (Imag-iT Fixation/Permeabilization Kit, Invitrogen) according to the manufacturer’s instructions. .. The colocalization coefficient of mCherry-KRT72 or control signals with HIV-1 core signals from INmNG (VSV-G-pseudotyped HIV-1) or anti-p24 antibody (WT HIV-1) staining was calculated by the colocalization module of Carl Zeiss Micro-imaging Zen software Blue version 3.3. ..

    Article Title: Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection
    Article Snippet: The coverslips were washed and then mounted to the cover slide with an antifade mounting medium (Absin, Cat# abs9234) and left to cure for 24 h. Images were taken on a Zeiss LSM 880 confocal microscope at × 40 magnification. .. Colocalization was measured using the colocalization module in Zen Blue (v2.5, Zen blue edition, Zeiss, Germany) software. ..

    Article Title: Novel protein complexes containing autophagy and UPS components regulate proteasome-dependent PARK2 recruitment onto mitochondria and PARK2-PARK6 activity during mitophagy
    Article Snippet: .. Colocalization coefficient of mitochondria with LC3, PSMA7, ATG5-12, PARK6 or PARK2 was analyzed using colocalization tool from Zen software (LSM710; Carl Zeiss, Inc., Germany) and then ordinary one-way ANOVA statistical analysis were performed using Prism 8 software for interpretation of the data. ..

    Article Title: APOE- ε4-induced Fibronectin at the blood-brain barrier is a conserved pathological mediator of disrupted astrocyte-endothelia interaction in Alzheimer’s disease
    Article Snippet: .. For fluorescence intensity colocalization, Manders colocalization coefficient ( ) was calculated using ZEN software colocalization module (blue edition, version 3.2, Carl Zeiss, Jena, Germany). ..

    Article Title: Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica.
    Article Snippet: .. Scatter plots of immunofluorescence images from Fig 3A were generated using the “Colocalization” tool of Zen software (Carl Zeiss, Germany) between anti-HA and antiBiP in TSPAN12-HA (upper panel) and TSPAN13-HA (middle panel) and TBP55-HA (lower panel) respectively. ..

    Article Title: S-palmitoylation determines TMEM55B-dependent positioning of lysosomes.
    Article Snippet: .. Single cells were framed and analyzed using the Zen 3.1 (blue edition) software ‘Colocalization’ module (Carl Zeiss Microscopy GmbH, Germany). ..

    other:

    Article Title: Tumor-derived erythropoietin acts as an immunosuppressive switch in cancer immunity
    Article Snippet: Plasma EPO levels were determined using the Mouse Erythropoietin/EPO Quantikine ELISA Kit (R&D Systems).

    Fluorescence:

    Article Title: APOE- ε4-induced Fibronectin at the blood-brain barrier is a conserved pathological mediator of disrupted astrocyte-endothelia interaction in Alzheimer’s disease
    Article Snippet: .. For fluorescence intensity colocalization, Manders colocalization coefficient ( ) was calculated using ZEN software colocalization module (blue edition, version 3.2, Carl Zeiss, Jena, Germany). ..

    Immunofluorescence:

    Article Title: Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica.
    Article Snippet: .. Scatter plots of immunofluorescence images from Fig 3A were generated using the “Colocalization” tool of Zen software (Carl Zeiss, Germany) between anti-HA and antiBiP in TSPAN12-HA (upper panel) and TSPAN13-HA (middle panel) and TBP55-HA (lower panel) respectively. ..

    Generated:

    Article Title: Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica.
    Article Snippet: .. Scatter plots of immunofluorescence images from Fig 3A were generated using the “Colocalization” tool of Zen software (Carl Zeiss, Germany) between anti-HA and antiBiP in TSPAN12-HA (upper panel) and TSPAN13-HA (middle panel) and TBP55-HA (lower panel) respectively. ..



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    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the <t>colocalization.</t> B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.
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    Image Search Results


    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    doi: 10.1371/journal.ppat.1002575

    Figure Lengend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at selected times and spleens were collected and examined by immunohistofluorescence. A , Immunoflurescence analyses of F4/80, MHC-II, and actin expression in spleen of mice at 24 h and 120 h after mCherry-Br infection. Yellow circles and arrowheads indicate the presence of the bacterium. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. B , Colocalization analysis of F4/80− and MHCII-expressing cells in spleen of mice before and 120 h after mCherry-Br infection. Scale bar = 50 and 200 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

    Techniques: Injection, Immunohistofluorescence, Expressing, Infection

    Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    doi: 10.1371/journal.ppat.1002575

    Figure Lengend Snippet: Wild-type C57BL/6 mice were injected i.p. with PBS or 10 8 CFU of mCherry-Br. Mice were sacrificed at 120 h p.i. and spleens were collected and examined by immunohistofluorescence. A , Immunofluorescence analysis of Ly-6G, CD90.2, CD11b, CD11c, F4/80, and MHC-II expressing cells and mCherry-Br. B , Percentage of iNOS + cells that colocalize with CD11b-, and CD11c-expressing cells. Numbers in B indicate the percentage of colocalizing cells in the upper panel. Images represent a single granuloma. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

    Techniques: Injection, Immunohistofluorescence, Immunofluorescence, Expressing

    Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    doi: 10.1371/journal.ppat.1002575

    Figure Lengend Snippet: Colocalization by immunohistofluorescence of CD11c ( A ), F4/80 ( B ), Ly-6G ( C ) and iNOS expressing cells with mCherry-Br signal in liver from 120 h mCherry-Br infected wild-type and MyD88 −/− C57BL/6. Mice were injected i.p. with 10 8 CFU of mCherry-Br. Panels are color-coded with the text for the antigen or mCherry-Br examined. Scale bar = 50 µm, as indicated. Data are representative of at least 3 independent experiments.

    Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

    Techniques: Immunohistofluorescence, Expressing, Infection, Injection

    Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Journal: PLoS Pathogens

    Article Title: In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice

    doi: 10.1371/journal.ppat.1002575

    Figure Lengend Snippet: Wild-type and Il-12p40 −/− BALB/c mice were injected i.p. with PBS or mCherry-Br, as indicated. Mice were sacrificed 5 or 12 days p.i. and spleens were collected and examined by immunohistofluorescence. A & B , Colocalisation of mCherry-Br with MOMA-1, CD11c, CD205 expressing cells in the spleen of IL-12 −/− BALB/c mice infected with 10 6 CFU 12 days p.i.. C , Higher magnification view of CD11c-expressing cells and mCherry-Br in w.p. of wild type BALB/c mice infected with 10 8 CFU 5 days p.i.. Panels are color-coded with the text for the antigen or mCherry-Br examined as well as the colocalization. Scale bar = 200 and 50 µm, as indicated. r.p.: red pulp; w.p.: white pulp, m.z.: marginal zone. Data are representative of at least 3 independent experiments.

    Article Snippet: Colocalization between two stainings was analyzed using the AxioVision Colocalization module (Zeiss).

    Techniques: Injection, Immunohistofluorescence, Expressing, Infection