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elisa kits  (R&D Systems)


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    Structured Review

    R&D Systems elisa kits
    Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mdc/Mouse+CCL22%2FMDC+DuoSet+ELISA/pm36189368-101-0-5
    Average 93 stars, based on 11 article reviews
    elisa kits - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Psychological stress creates an immune suppressive environment in the lung that increases susceptibility of aged mice to Mycobacterium tuberculosis infection.
    Article Snippet: Turbo-TMB-ELISA Substrate (Thermal Fisher Scientific was used for detection. .. ELISA kits were purchased from R&D Systems (CCL2 [DY439], IL-1b [DY421], TNF-a [DY410], IL-12p70 [DY419], GM-CSF [DY415], M-CSF [DY416], IFN-g [DY485], IL-17 [DY421] or IL-10 (MABTECH 3432). .. Aliquots of BAL fluid were added to individual ELISA plate wells (Nunc MaxiSorp plates; Thermal Fisher Scientific).

    Article Title: DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation.
    Article Snippet: Biotinylated G-actin (Cytoskeleton) reconstituted at 1 mg/mL with sterile water was mixed in a 1:1 molar ratio with nonbiotinylated G-actin and polymerised with G- and F-buffer for ≥1 h at RT. .. Unless otherwise stated, cultured supernatant analytes were quantified using mouse CCL17/TARC, CCL22/MDC, IL-12 p40, TNF-α DuoSet ELISA kits (R&D Systems) according to the manufacturer’s instructions. .. Biotinylated G-actin (Cytoskeleton) reconstituted at 1 mg/mL with sterile water was mixed in a 1:1 molar ratio with nonbiotinylated G-actin and polymerised with G- and F-buffer for ≥1 h at RT.

    Article Title: DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation
    Article Snippet: Beads were washed with 1% BSA in PBS at ≥10,000× g . DNGR-1L-OVA beads were made by first incubating washed (with 1% BSA in PBS at ≥10,000× g ) 2 μm streptavidin non-fluorescent beads with 2 mg/mL ovalbumin (OVA) biotinylated using the DSB-X-biotinylation kit (Thermo Fisher Scientific) for 30 min on ice. .. Unless otherwise stated, cultured supernatant analytes were quantified using mouse CCL17/TARC, CCL22/MDC, IL-12 p40, TNF-α DuoSet ELISA kits (R&D Systems) according to the manufacturer’s instructions. ..

    Article Title:
    Article Snippet: .. Cytokine and chemokine quantification was performed from culture supernatants, peritoneal lavage or serum, according to the manufacturer instructions: IL-12 p40 Mouse uncoated ELISA kit (Invitrogen, 88–7120-88), Mouse CCL5/Rantes DuoSet ELISA (R&D Systems, DY478), Mouse TNF-alpha DuoSet ELISA (R&D Systems, DY410), Mouse Interferon-gamma DuoSet ELISA (R&D Systems, DY485), and Mouse CCL22 DuoSet ELISA (R&D Systems, DY439). .. Statistical analysis was performed using GraphPad Prism 9.5 for macOS (GraphPad Software).

    Article Title: Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma
    Article Snippet: .. Mouse MCP-1 ELISA (CCL2) (432704, Biolegend, San Diego, CA, United States), Mouse CCL22/MDC DuoSet ELISA (DY439, R&D Systems, MN, United States), Mouse CXCL9 ELISA Kit (ab203364, Abcom, Atlanta, GA, United States), Mouse IP-10 ELISA Kit (CXCL10) (ab260067, Abcom), were used for chemokine analysis. ..

    Article Title: Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma.
    Article Snippet: .. Mouse MCP-1 ELISA (CCL2) (432704, Biolegend, San Diego, CA, United States), Mouse CCL22/ Frontiers in Pharmacology frontiersin.org02 MDC DuoSet ELISA (DY439, R&D Systems, MN, United States), Mouse CXCL9 ELISA Kit (ab203364, Abcom, Atlanta, GA, United States), Mouse IP-10 ELISA Kit (CXCL10) (ab260067, Abcom), were used for chemokine analysis. ..

    Article Title:
    Article Snippet: Mouse CCL5/RANTES DuoSet ELISA , R&D Systems , DY478. .. Mouse CCL22 DuoSet ELISA , R&D Systems , DY439. .. Mouse IFN-gamma DuoSet ELISA , R&D Systems , DY485.

    Cell Culture:

    Article Title: DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation.
    Article Snippet: Biotinylated G-actin (Cytoskeleton) reconstituted at 1 mg/mL with sterile water was mixed in a 1:1 molar ratio with nonbiotinylated G-actin and polymerised with G- and F-buffer for ≥1 h at RT. .. Unless otherwise stated, cultured supernatant analytes were quantified using mouse CCL17/TARC, CCL22/MDC, IL-12 p40, TNF-α DuoSet ELISA kits (R&D Systems) according to the manufacturer’s instructions. .. Biotinylated G-actin (Cytoskeleton) reconstituted at 1 mg/mL with sterile water was mixed in a 1:1 molar ratio with nonbiotinylated G-actin and polymerised with G- and F-buffer for ≥1 h at RT.

    Article Title: DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation
    Article Snippet: Beads were washed with 1% BSA in PBS at ≥10,000× g . DNGR-1L-OVA beads were made by first incubating washed (with 1% BSA in PBS at ≥10,000× g ) 2 μm streptavidin non-fluorescent beads with 2 mg/mL ovalbumin (OVA) biotinylated using the DSB-X-biotinylation kit (Thermo Fisher Scientific) for 30 min on ice. .. Unless otherwise stated, cultured supernatant analytes were quantified using mouse CCL17/TARC, CCL22/MDC, IL-12 p40, TNF-α DuoSet ELISA kits (R&D Systems) according to the manufacturer’s instructions. ..



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    Image Search Results


    Regulatory effect of DML on autophagy in LPS-induced RAW264.7 cells. (A) Autophagosomes were determined by MDC staining under a confocal microscope. Scale bars, 25 μm. (B) The fluorescence intensity of autophagosomes by MDC staining per image in (A). The mRNA expression of LC3 B (C) and p62 (D). (E) LC3 I, LC3 II, and p62 protein levels. Internal reference was β-actin. (F) The statistical analysis of LC3 II/LC3 I and p62. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** P < 0.001, compared with the control group. # p < 0.05 and ## p < 0.01, compared with the LPS group. § p < 0.05, §§§ p < 0.001, and §§§§ p < 0.0001, compared with the CQ group. @ p < 0.05 and @@@ p < 0.001, compared with the CQ + LPS group.

    Journal: ACS Omega

    Article Title: Des-O-Methyllasiodiplodin Isolated from Xanthostemon chrysanthus Ameliorates Inflammation by Regulating Autophagy

    doi: 10.1021/acsomega.5c12134

    Figure Lengend Snippet: Regulatory effect of DML on autophagy in LPS-induced RAW264.7 cells. (A) Autophagosomes were determined by MDC staining under a confocal microscope. Scale bars, 25 μm. (B) The fluorescence intensity of autophagosomes by MDC staining per image in (A). The mRNA expression of LC3 B (C) and p62 (D). (E) LC3 I, LC3 II, and p62 protein levels. Internal reference was β-actin. (F) The statistical analysis of LC3 II/LC3 I and p62. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** P < 0.001, compared with the control group. # p < 0.05 and ## p < 0.01, compared with the LPS group. § p < 0.05, §§§ p < 0.001, and §§§§ p < 0.0001, compared with the CQ group. @ p < 0.05 and @@@ p < 0.001, compared with the CQ + LPS group.

    Article Snippet: Reactive Oxygen Species Assay Kit, DAF-FM DA probe, Autophagy Staining Assay Kit with MDC, RIPA lysate buffer, BCA protein determination kit, and All-purpose Powerful Antigen Retrieval Solution were from Beyotime Institute of Biotechnology (Shanghai, China).

    Techniques: Staining, Confocal, Microscopy, Fluorescence, Expressing, Analysis, Control