Journal: Chemical Engineering Journal

Article Title: Microfluidic fabrication of lipid nanoparticles for co-delivery of siRNA and hydroxychloroquine: An engineered theranostic platform for enhanced breast cancer treatment

doi: 10.1016/j.cej.2025.160172

Figure Lengend Snippet: Fig. 3. Effect of various treatments on cell proliferation. Screening of human- (A) and mouse- (B) derived candidate siRNA sequences. (C) Cell counting kit-8 (CCK-8) assay to evaluate anti-proliferative results against 4T1 cells. (D) Crystalline violet staining to visualize agent-treated cell colonies. (E) Ultraviolet absorption of crystalline violet eluents from stained colonies (n = 3). *p<0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The LysoTracker Green and Autophagy Staining Assay Kit with MDC were obtained from Beyotime (Shanghai, China).

Techniques: Derivative Assay, Cell Counting, CCK-8 Assay, Staining

Journal: The Journal of Clinical Investigation

Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin, or esophageal injury

doi: 10.1172/JCI173649

Figure Lengend Snippet: ( A ) Experimental timeline. Female C57BL/6 mice orthotopically injected with e0771 on day 0 and when tumors reached 2–5 mm in size mice were irradiated with a whole-thorax irradiation dose of 20 Gy and treated with either TLY012, ONC201, or the combination ( n = 3–4 per treatment per group). (Created in BioRender. Strandberg J. 2024. https://BioRender.com/c65v064. ) ( B and C ) Tumors were removed after mice were euthanized 18 days after cell injection, and weight and volume were calculated. ( D ) Pulse oximetry readings before radiation and 9 days after radiation showed oxygen saturation more conserved in the TLY012-treated group compared with irradiated controls. Values are mean ± SEM. ( E ) Statistical analysis of cytokine fold change showed significant decrease in levels of MDC/CCL22 ( P = 0.035) in comparison with irradiated controls (1-way ANOVA with Tukey’s post hoc test).

Article Snippet: Seven-week-old Trail –/– female mice received a single thoracic x-ray irradiation dose of 20 Gy with shielding of other organs and were treated with either 20 μg of anti-CCL22 antibody (AF439, R&D Systems Inc.) dissolved in 500 μL of sterile normal saline via i.p. injection every other day for 2 weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems Inc.) dissolved in 500 μL of normal saline via i.p. injection every other day for 2 weeks ( n = 5 per treatment per group).

Techniques: Injection, Irradiation, Comparison

Journal: The Journal of Clinical Investigation

Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin, or esophageal injury

doi: 10.1172/JCI173649

Figure Lengend Snippet: ( A ) Experimental timeline of Trail –/– female mice treated with 20 μg of anti-CCL22 in 500 μL of saline or 20 μg of goat IgG in 500 μL of saline every other day for 2 weeks ( n = 5 per treatment per group). (Created in BioRender. Strandberg J. 2024. https://BioRender.com/j52q830. ) ( B ) H&E images of each mouse 2 weeks after thoracic irradiation (original magnification, ×20). Scale bar: 100 μm. ( C ) Quantification of inflammatory scores provided by a blinded pathologist showed decrease in inflammation in Trail –/– female mice treated with anti-CCL22 ( n = 5 per treatment per group) but not to a significant extent (1-tailed Mann-Whitney test) ( P = 0.1032). Values are mean ± SEM.

Article Snippet: Seven-week-old Trail –/– female mice received a single thoracic x-ray irradiation dose of 20 Gy with shielding of other organs and were treated with either 20 μg of anti-CCL22 antibody (AF439, R&D Systems Inc.) dissolved in 500 μL of sterile normal saline via i.p. injection every other day for 2 weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems Inc.) dissolved in 500 μL of normal saline via i.p. injection every other day for 2 weeks ( n = 5 per treatment per group).

Techniques: Saline, Irradiation, MANN-WHITNEY