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maxi-script sp6/t7 vitro transcription kit  (Thermo Fisher)


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    Thermo Fisher maxi-script sp6/t7 vitro transcription kit
    Maxi Script Sp6/T7 Vitro Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxi+script+sp6+kit/pmc02613866-80-21-27
    Average 90 stars, based on 1 article reviews
    maxi-script sp6/t7 vitro transcription kit - by Bioz Stars, 2026-11
    90/100 stars

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    In Vitro:

    Article Title: Knockout of a single Pax6 gene ( toy but not ey ) leads to compound eye deficiency and small head in honeybees
    Article Snippet: PCR products were purified by a PCR clean-up (Axygen) kit. .. In vitro transcription was performed with the MAXIscript SP6/T7 RNA in vitro transcription kit (Ambion, USA) according to the manufacturer’s instruction. .. The Cas9 protein (TrueCutTM Cas9 Protein v2) was purchased from Thermo Fisher Scientific (Shanghai, China).

    other:

    Article Title: Argonaute 2 targets viral transcripts but not genomes of RNA viruses during antiviral RNA interference in Drosophila.
    Article Snippet: Primer sequences are provided in S1 Table. dsRNA constructions and treatments In vitro transcription was done using T7/SP6 MEGAscript Kits (Ambion), following the manufacturer’s instructions.

    Article Title: IRE1α determines ferroptosis sensitivity through regulation of glutathione synthesis.
    Article Snippet: Briefly, RNA substrates were generated by in vitro transcription using the MEGAscript T7 Transcription or MAXIscript SP6/T7 Transcription Kit (Invitrogen) with DNA templates produced through either linearizing plasmids containing the mRNA CDS following an SP6/T7 promoter or annealing chemically synthesized complementary singlestranded DNA oligonucleotides which contain a T7 promoter sequence (Sigma Aldrich Genosys).

    Article Title: The pseudogene GBP1P1 suppresses influenza A virus replication by acting as a protein decoy for DHX9
    Article Snippet: For double FISH, primary and Alexa Fluor secondary antibodies (Invitrogen) were added.

    Article Title: LUCAT1 Activates the Malignant Phenotypes of Lung Cancer Cells via Regulating P53 Ubiquitination
    Article Snippet: Briefly, LUCAT1 and its antisense RNA were biotinylated by using MEGAscriptTM T7/SP6 Transcription Kit (Life Technologies, USA) according to the manufacturer’s instructions.

    Article Title: The loach haplotype-resolved genome and the identification of Mex3a involved in fish air breathing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Mex3a Abclonal Technology Cat# A22102; RRID: AB_3662045 Rabbit polyclonal anti-Tbx20 Abclonal Technology Cat# A15994; RRID:AB_2763434 Rabbit polyclonal anti-Mmp9 Abclonal Technology Cat# A0289; RRID:AB_2757101 Rabbit polyclonal anti-Mmp13 Abclonal Technology Cat# A1606; RRID:AB_2763499 Rabbit polyclonal anti-Mapkapk2 Abclonal Technology Cat# A22183; RRID: AB_3662047 HRP-conjugated IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific antibody Proteintech Company Cat# SA00001-7L; RRID:AB_2890988 Rabbit polyclonal anti-Prok2 Abclonal Technology Cat# A6705; RRID:AB_2767288 Rabbit polyclonal anti-Prokr1 Cusabio Technology Cat# CSB-PA003812; RRID: AB_3662046 Anti-Gapdh ABclonal Technology Cat# AC001; RRID:AB_2619673 Rabbit polyclonal anti-CD146 ABclonal Technology Cat# A13927; RRID:AB_2760779 Bacterial and virus strains pcDNA3.1(+)eGFP Wuhan Tianyi Huayu Gene Technology N/A Critical commercial assays MAXIscript SP6/T7 Kit Thermo Fisher Scientific Cat# AM1320 Protein A + G Agarose (Fast Flow, for IP) kit Beyotime Biotechnology Company Cat# P2055-2mL RFectPMsiRNA/miRNA transfection reagent Changzhou Biogenerating Biotechnology N/A Hieff MutTM Site-Directed Mutagenesis Kit Yeasen Biotechnology Cat# 11003ES10 Deposited data Loach haplotype-Mchra This paper GWHERQE00000000 Loach haplotype-Mchrb This paper GWHERQD00000000 RNA_seq: WT and KO loach intestine tissues This paper PRJNA1091403 Experimental models: Cell lines HUVEC Wuhan Pricella Biotechnology CL-0675 Experimental models: Organisms/strains Loach This paper N/A AB strain zebrafish China Zebrafish Resource Center CZ1 Tg (fli1a:EGFP) transgenic zebrafish China Zebrafish Resource Center CZ-ID:CZ55 Oligonucleotides Primers This paper N/A Software and algorithms GraphPad Prism 8.0 software GraphPad Software Inc., San Diego, CA, USA N/A IBM SPSS Statistics v 26.0 SPSS Inc., USA N/A Hifiasm Li et al.60 https://github.com/chhylp123/hifiasm 3D-DNA Dudchenko et al.61 https://github.com/aidenlab/3d-dna Racon Vaser et al.62 https://github.com/isovic/racon BUSCO Seppey et al.63 https://busco.ezlab.org/ TRF Benson64 https://tandem.bu.edu/trf/trf.html RepeatModeler Flynn et al.65 https://github.com/Dfam-consortium/ RepeatModeler (Continued on next page) e1 Cell Genomics 4, 100670, October 9, 2024

    Article Title: Transient expression of the neuropeptide galanin modulates peripheral‑to‑central connectivity in the somatosensory thalamus during whisker development in mice.
    Article Snippet: Radiolabeled probes were prepared by in vitro transcription using the MAXIScript Sp6/T7 kit (Applied Biosystems) and [α35S]UTP (NEG039H, Perkin Elmer).

    Article Title: The pseudogene GBP1P1 suppresses influenza A virus replication by acting as a protein decoy for DHX9.
    Article Snippet: For double FISH, primary and Alexa Fluor secondary Month XXXX Volume 0 Issue 0 10.1128/jvi.00738-24 4 h ttp s: //j ou rn al s. as m .o rg /jo ur na l/j vi o n 05 J ul y 20 24 b y 24 09 :4 0f 2: 20 47 :6 36 3: da :4 5f 1: b2 25 :5 fa d. antibodies (Invitrogen) were added



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