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sp6 or t3/t7 maxi script kits  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sp6 or t3/t7 maxi script kits
    Sp6 Or T3/T7 Maxi Script Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxi+script+sp6+kit/pm12781692-92-10-16
    Average 90 stars, based on 1 article reviews
    sp6 or t3/t7 maxi script kits - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Phylogenetic Diversity in Core Region of Hepatitis C Virus Genotype 1a as a Factor Associated with Fibrosis Severity in HIV-1-Coinfected Patients
    Article Snippet: Plasmid inserts were amplified using pGEM®-T-specific primers T7 and SP6 and subjected to double stranded sequence analysis with dye-labeled dideoxy terminators with both T7 and SP6 primers (genetic analyzer 3500xL, Applied Biosystems®, Life Technologies, USA).

    Article Title: A detailed kinetic model of Eastern equine encephalitis virus replication in a susceptible host cell
    Article Snippet: Transcription from the SP6 (Invitrogen) promoter yielded negative-sense full-length genome while transcription from the T7 promoter (Invitrogen) yielded positive sense full length genome.

    Article Title: The Basal Ciliary but not Cytosol PKA Specifically Regulates HH Pathway Downstream of Smoothened
    Article Snippet: The mRNA was produced using either Sp6 or T3 Invitrogen Ambion mMessagemMachine® Kit according to the promoter in the constructs following the instructions, then purified mRNA were stocked in -80°C for further use.

    In Vitro:

    Article Title: Developmental stage-specific proteome analysis of the legume pod borer Maruca vitrata provides insights on relevant proteins.
    Article Snippet: The spotted pod borer, Maruca vitrata (Lepidoptera: Crambidae) is a destructive insect pest that inflicts significant productivity losses on important leguminous crops.. Unravelling insect proteomes is vital to comprehend their fundamental molecular mechanisms.. This research delved into the proteome profiles of four distinct stages -three larval and pupa of M. vitrata, utilizing LC-MS/MS label-free quantification-based methods.

    Article Title: Wnt/β-catenin signaling promotes posterior axial regeneration in non-regenerative tissue of the annelid Capitella teleta
    Article Snippet: .. Anti-sense digoxigenin-labeled (Sigma-Aldrich) riboprobes for all genes were synthesized in vitro with the SP6 or T7 MEGAscript kit (Ambion, Inc., Austin, TX, USA). ..

    Construct:

    Article Title: Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development
    Article Snippet: .. For mRNA transcription, PCRTopoII or pBSII vectors containing the cDNAs of full-length moe , epb4.1l5 long , epb4.1l5 short , or myc-tagged fusions of the following constructs; epb4.1l5 long , epb4.1l5 FERM (1–346 N-terminal amino acids), epb4.1l51 long_ΔPBD (epb4.1l5 long with the four C'terminal TTEL deleted), epb4.1l51 short+long_PBD (short C-terminal AAMTEI replaced with LLTTEL) or epb4.1l5 long+short_PBD (long C-terminal LLTTEL replaced with AAMTEI), were linearized by NotI or PspOMI restriction digest, and transcribed with the Sp6 or T7 Message Machine transcription kit (Ambion). ..

    Restriction Assay:

    Article Title: Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development
    Article Snippet: .. For mRNA transcription, PCRTopoII or pBSII vectors containing the cDNAs of full-length moe , epb4.1l5 long , epb4.1l5 short , or myc-tagged fusions of the following constructs; epb4.1l5 long , epb4.1l5 FERM (1–346 N-terminal amino acids), epb4.1l51 long_ΔPBD (epb4.1l5 long with the four C'terminal TTEL deleted), epb4.1l51 short+long_PBD (short C-terminal AAMTEI replaced with LLTTEL) or epb4.1l5 long+short_PBD (long C-terminal LLTTEL replaced with AAMTEI), were linearized by NotI or PspOMI restriction digest, and transcribed with the Sp6 or T7 Message Machine transcription kit (Ambion). ..

    Generated:

    Article Title: Characterization of eight new Hydractinia i-cell markers reveals underlying heterogeneity in the adult pluripotent stem cell population
    Article Snippet: .. Digoxigenin (DIG)-labeled and/or Fluoresecein-labeled riboprobes for selected genes were generated with the SP6 or T7 MEGAscript kit (Cat #AM1334, #AM1330, Ambion, Inc., Austin, TX, USA) using the open reading frames (ORFs) of cloned Hydractinia genes as template and following manufacturer’s recommendations. ..

    Clone Assay:

    Article Title: Characterization of eight new Hydractinia i-cell markers reveals underlying heterogeneity in the adult pluripotent stem cell population
    Article Snippet: .. Digoxigenin (DIG)-labeled and/or Fluoresecein-labeled riboprobes for selected genes were generated with the SP6 or T7 MEGAscript kit (Cat #AM1334, #AM1330, Ambion, Inc., Austin, TX, USA) using the open reading frames (ORFs) of cloned Hydractinia genes as template and following manufacturer’s recommendations. ..

    Synthesized:

    Article Title: Wnt/β-catenin signaling promotes posterior axial regeneration in non-regenerative tissue of the annelid Capitella teleta
    Article Snippet: .. Anti-sense digoxigenin-labeled (Sigma-Aldrich) riboprobes for all genes were synthesized in vitro with the SP6 or T7 MEGAscript kit (Ambion, Inc., Austin, TX, USA). ..



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