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massarray iplextm gold genotyping platform  (Sequenom)

 
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    Sequenom massarray iplextm gold genotyping platform
    Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed <t>genotyping</t> of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic <t>SNP,</t> denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.
    Massarray Iplextm Gold Genotyping Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray+iplextm+gold/sequenom+massarray/pmc03025900-214-1-7
    Average 90 stars, based on 1 article reviews
    massarray iplextm gold genotyping platform - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "DNA sequence polymorphisms within the bovine guanine nucleotide-binding protein Gs subunit alpha (Gsα)-encoding ( GNAS ) genomic imprinting domain are associated with performance traits"

    Article Title: DNA sequence polymorphisms within the bovine guanine nucleotide-binding protein Gs subunit alpha (Gsα)-encoding ( GNAS ) genomic imprinting domain are associated with performance traits

    Journal: BMC Genetics

    doi: 10.1186/1471-2156-12-4

    Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed genotyping of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic SNP, denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.
    Figure Legend Snippet: Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed genotyping of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic SNP, denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.

    Techniques Used: Sequencing, Expressing

    Related Articles

    Mass Spectrometry:

    Article Title: Genetic and Functional Dissection of the NFKB2 Gene: Implications for Milk Fatty Acid Biosynthesis in Dairy Cattle
    Article Snippet: The PCR amplification products were bi‐directionally sequenced using an ABI3730XL DNA analyzer (Applied Biosystems, Foster, CA, USA) to identify potential polymorphisms. .. Subsequently, 1065 cows were genotyped on each identified SNP by using the matrix‐assisted laser‐desorption/ionization time of flight mass spectrometry (MALDI‐TOF MS, Sequenom MassARRAY, Bioyong Technologies Inc., HK). .. Using SAS 9.2, the phenotype–genotype association analyses between the identified SNP and 24 milk FA traits was conducted with the mixed animal model below, Y ijklm = μ + G i + h j + l k + a l + b × M m + e ijklm In which, Y ijklm was the phenotypic value of each milk FA trait; μ was the overall mean; G i was the fixed effect corresponding to the genotype combination of individual i ; h j ( j = 1–23) and l k ( k = 1–4) were the fixed effect of farm j and stage of lactation l , respectively; a l was the random polygenic effect; M m ( m = 1–293) was the fixed effect of age at calving m ; b was the regression coefficient of covariate M ; and e ijklm was the random residual.

    Article Title: FCRL3 genetic variants drive autoimmune pathogenesis in multiple sclerosis and neuromyelitis optica spectrum disorders
    Article Snippet: .. This was carried out using the matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) platform (MassArray TM, Sequenom Inc., San Diego, CA, USA), following a previously established method ( ). .. PCR and extension primers were designed using MassArray Assay Design 3.1 software (Sequenom, San Diego, CA, USA) ( ).

    Mutagenesis:

    Article Title:
    Article Snippet: .. RAS/RAF mutation status of the ascites and matching tumor biopsies were determined by Sanger sequencing (ascites) and Sequenom analysis (tumor). ..

    Sequencing:

    Article Title:
    Article Snippet: .. RAS/RAF mutation status of the ascites and matching tumor biopsies were determined by Sanger sequencing (ascites) and Sequenom analysis (tumor). ..

    Polymerase Chain Reaction:

    Article Title: Method for treatment of hypertension
    Article Snippet: .. Each sample will be analyzed using 2 different methodologies, the Sequenom MassArray genotyping platform and classical PCR and gel sizing to determine insertion/deletion status. .. The Sequenom MassArray genotyping platform will be used to analyze the following sites-rs1042713, rs1042714, rs1801252, rs1801253, rs4961, rs2228576, rs1529927, rs1159744, rs2107614, rs2277869, rs12750834, rs5051, rs699, rs7079 and rs5186.

    Multiplex Assay:

    Article Title: Evidence of Genetic Isolation and Differentiation Among Historically Fragmented British Populations of Common Juniper, Juniperus communis L.
    Article Snippet: .. Of these 175 loci, 80 were selected for two multiplex Sequenom assays (Bradić et al. ), which ultimately provided data at 74 SNP loci for all samples (Table ). ..

    other:

    Article Title: Compositions and methods for treating cancer
    Article Snippet: Detection of one or more mutations may also utilize an array of probes (also referred to as a “DNA chip” assay, e.g. a GeneChip assay-Affymetrix, Santa Clara, CA).



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    Image Search Results


    Comparison of allele frequencies 1 estimated using high-throughput sequencing vs. genotyping for 43 SNPs across two pools of 75 dairy cattle divergent for calving interval (CIV) . 1 Actual genotype frequencies calculated from Sequenom ® MassARRAY data obtained from previous studies [ , , , , ].

    Journal: BMC Genomics

    Article Title: Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples

    doi: 10.1186/1471-2164-13-16

    Figure Lengend Snippet: Comparison of allele frequencies 1 estimated using high-throughput sequencing vs. genotyping for 43 SNPs across two pools of 75 dairy cattle divergent for calving interval (CIV) . 1 Actual genotype frequencies calculated from Sequenom ® MassARRAY data obtained from previous studies [ , , , , ].

    Article Snippet: Estimated allele frequencies of a selection of SNPs from the sequence capture target enrichment and sequencing of pooled samples were compared to actual allele frequencies generated using Sequenom ® MassARRAY iPLEXTM gold assay.

    Techniques: Comparison, Next-Generation Sequencing

    Comparison between allele frequencies generated using Sequenom ® MassARRAY and Illumina GAIIx technologies.

    Journal: BMC Genomics

    Article Title: Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples

    doi: 10.1186/1471-2164-13-16

    Figure Lengend Snippet: Comparison between allele frequencies generated using Sequenom ® MassARRAY and Illumina GAIIx technologies.

    Article Snippet: Estimated allele frequencies of a selection of SNPs from the sequence capture target enrichment and sequencing of pooled samples were compared to actual allele frequencies generated using Sequenom ® MassARRAY iPLEXTM gold assay.

    Techniques: Comparison, Generated

    Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed genotyping of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic SNP, denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.

    Journal: BMC Genetics

    Article Title: DNA sequence polymorphisms within the bovine guanine nucleotide-binding protein Gs subunit alpha (Gsα)-encoding ( GNAS ) genomic imprinting domain are associated with performance traits

    doi: 10.1186/1471-2156-12-4

    Figure Lengend Snippet: Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed genotyping of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic SNP, denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.

    Article Snippet: All SNP genotyping was performed commercially by Sequenom Inc. (San Diego, CA, USA; http://www.sequenom.com using their proprietary MassARRAY iPLEXTM Gold genotyping platform.

    Techniques: Sequencing, Expressing