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maldi-tof analysis on the sequenom massarray platform  (Sequenom)

 
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    Sequenom maldi-tof analysis on the sequenom massarray platform
    Maldi Tof Analysis On The Sequenom Massarray Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray%C2%AE+maldi-tof+platform/sequenom+massarray/pmc09827130-40-8-8
    Average 90 stars, based on 1 article reviews
    maldi-tof analysis on the sequenom massarray platform - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Mass Spectrometry:

    Article Title: Genetic and Functional Dissection of the NFKB2 Gene: Implications for Milk Fatty Acid Biosynthesis in Dairy Cattle
    Article Snippet: The PCR amplification products were bi‐directionally sequenced using an ABI3730XL DNA analyzer (Applied Biosystems, Foster, CA, USA) to identify potential polymorphisms. .. Subsequently, 1065 cows were genotyped on each identified SNP by using the matrix‐assisted laser‐desorption/ionization time of flight mass spectrometry (MALDI‐TOF MS, Sequenom MassARRAY, Bioyong Technologies Inc., HK). .. Using SAS 9.2, the phenotype–genotype association analyses between the identified SNP and 24 milk FA traits was conducted with the mixed animal model below, Y ijklm = μ + G i + h j + l k + a l + b × M m + e ijklm In which, Y ijklm was the phenotypic value of each milk FA trait; μ was the overall mean; G i was the fixed effect corresponding to the genotype combination of individual i ; h j ( j = 1–23) and l k ( k = 1–4) were the fixed effect of farm j and stage of lactation l , respectively; a l was the random polygenic effect; M m ( m = 1–293) was the fixed effect of age at calving m ; b was the regression coefficient of covariate M ; and e ijklm was the random residual.

    Article Title: FCRL3 genetic variants drive autoimmune pathogenesis in multiple sclerosis and neuromyelitis optica spectrum disorders
    Article Snippet: .. This was carried out using the matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) platform (MassArray TM, Sequenom Inc., San Diego, CA, USA), following a previously established method ( ). .. PCR and extension primers were designed using MassArray Assay Design 3.1 software (Sequenom, San Diego, CA, USA) ( ).

    Mutagenesis:

    Article Title:
    Article Snippet: .. RAS/RAF mutation status of the ascites and matching tumor biopsies were determined by Sanger sequencing (ascites) and Sequenom analysis (tumor). ..

    Sequencing:

    Article Title:
    Article Snippet: .. RAS/RAF mutation status of the ascites and matching tumor biopsies were determined by Sanger sequencing (ascites) and Sequenom analysis (tumor). ..

    Polymerase Chain Reaction:

    Article Title: Method for treatment of hypertension
    Article Snippet: .. Each sample will be analyzed using 2 different methodologies, the Sequenom MassArray genotyping platform and classical PCR and gel sizing to determine insertion/deletion status. .. The Sequenom MassArray genotyping platform will be used to analyze the following sites-rs1042713, rs1042714, rs1801252, rs1801253, rs4961, rs2228576, rs1529927, rs1159744, rs2107614, rs2277869, rs12750834, rs5051, rs699, rs7079 and rs5186.

    Multiplex Assay:

    Article Title: Evidence of Genetic Isolation and Differentiation Among Historically Fragmented British Populations of Common Juniper, Juniperus communis L.
    Article Snippet: .. Of these 175 loci, 80 were selected for two multiplex Sequenom assays (Bradić et al. ), which ultimately provided data at 74 SNP loci for all samples (Table ). ..

    other:

    Article Title: Compositions and methods for treating cancer
    Article Snippet: Detection of one or more mutations may also utilize an array of probes (also referred to as a “DNA chip” assay, e.g. a GeneChip assay-Affymetrix, Santa Clara, CA).



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    ( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation <t>(MassArray)</t> are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® <t>MALDI-TOF</t> platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.
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    ( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation <t>(MassArray)</t> are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® <t>MALDI-TOF</t> platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.
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    ( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation <t>(MassArray)</t> are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® <t>MALDI-TOF</t> platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.
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    Image Search Results


    ( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation (MassArray) are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® MALDI-TOF platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.

    Journal: PLoS ONE

    Article Title: MicroRNA-200 Family Modulation in Distinct Breast Cancer Phenotypes

    doi: 10.1371/journal.pone.0047709

    Figure Lengend Snippet: ( A ) Schematic depiction of miR- 200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation (MassArray) are indicated by a black bar. Chromosomal location is indicated between brackets. ( B ) The DNA methylation levels across the regions shown in panel ( A ) were quantified in breast cancers by Sequenom MassArray® MALDI-TOF platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. ( C ) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.

    Article Snippet: The DNA methylation status of miR-200f loci in tumor samples were inspected using Sequenom MassArray® MALDI-TOF platform.

    Techniques: DNA Methylation Assay, Methylation, Expressing, CpG Methylation Assay