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macropatch electrodes  (Zeitz Instruments Vertriebs GmbH)


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    Zeitz Instruments Vertriebs GmbH macropatch electrodes
    Macropatch Electrodes, supplied by Zeitz Instruments Vertriebs GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macropatch/microelectrodes/pm12686574-53-0-13
    Average 90 stars, based on 1 article reviews
    macropatch electrodes - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Antagonist pharmacology of desensitizing and non-desensitizing nicotinic acetylcholine receptors in cockroach neurons.
    Article Snippet: Two a-bungarotoxin-sensitive nicotinic acetylcholine (ACh) receptor subtypes in neurons of the American cockroach have been identified as desensitizing (nAChD) and selectively inhibitable with 100 nM imidacloprid, and non-desensitizing (nAChN) and selectively inhibitable with 100 pM methyllycaconitine.. In this paper, the single-electrode voltage-clamp technique was used to measure concentration-response relations for the action of ACh and five antagonists on pharmacologically separated nAChD and nAChN receptors of acutely dissociated neurons from thoracic ganglia of the American cockroach.. A dual bath and U-tube perfusion system was used to achieve rapid application of ACh in the continued presence of antagonists, which was essential to accurately measure inhibition by rapidly-reversible antagonists.

    Article Title: Volume Microscopy
    Article Snippet: Microelectrodes with a resistance of 7–11 MΩ were made from 2 mm borosilicate glass capillaries with filament (GBF 200F 10, Science Products) using a DMZ Universal Puller (Zeitz instruments) at least 1 day before electroporation to reduce adhesion between electrode tip and cell surfaces.

    Article Title: Feedforward discharges couple the singing central pattern generator and ventilation central pattern generator in the cricket abdominal central nervous system
    Article Snippet: Sharp microelectrodes were pulled (DMZ-Universal Puller, Zeitz-Instruments, Martinsried, Germany) from thick-wall borosilicate glass capillaries (GC100F-10, Harvard Apparatus Ltd., Kent, UK), filled with 2 mol l −1 potassium acetate and resistances of 70–90 MΩ.

    Article Title: The Cav1.2 N terminus contains a CaM kinase site that modulates channel trafficking and function.
    Article Snippet: The L-type voltage-gated calcium channel Cav1.2 and the calcium-activated CaM kinase cascade both regulate excitation transcription coupling in the brain.. CaM kinase is known to associate with the C terminus of Cav1.2 in a region called the PreIQ-IQ domain, which also binds multiple calmodulin molecules.. Here we identify and characterize a second CaMKII binding site in the N terminus of Cav1.2 that is formed by a stretch of four amino residues (cysteine–isoleucine–serine–isoleucine) and which regulates channel expression and function.

    Article Title: An auditory feature detection circuit for sound pattern recognition
    Article Snippet: Sharp microelectrodes were pulled (DMZ-Universal Puller, Zeitz-Instruments) from borosilicate glass capillaries (GC100F-10, Harvard Apparatus Ltd.).

    Article Title: Functional characterization and physiological roles of the single Shaker outward K + channel in Medicago truncatula.
    Article Snippet: Patch-clamp pipettes were pulled using a DMZ-Universal Puller (Zeitz-Instruments GmbH, Germany) from borosilicate capillaries (GC150F-7.5; Phymep, France) and fire polished (by the DMZ-Universal Puller).

    Article Title: Hydrogen sulfide stimulates CFTR in Xenopus oocytes by activation of the cAMP/PKA signalling axis.
    Article Snippet: Chlorinated silver wires served as recording electrodes and were mounted into borosilicate glass capillaries (Hilgenberg, Malsfeld, Germany) with an outer diameter of 1.2 mm, which were pulled to microelectrodes with a DMZ universal puller (Zeitz-Instruments, Martinsried, Germany) and filled with 1 M KCl.

    Article Title: Selective actions of insecticides on desensitizing and non-desensitizing nicotinic acetylcholine receptors in cockroach (Periplaneta americana) neurons.
    Article Snippet: BACKGROUND: Insect desensitizing nicotinic acetylcholine (nAChD) receptors are desensitized by low concentrations of agonists, including neonicotinoid insecticides, but are essentially insensitive to spinosyns, while non-desensitizing nicotinic acetylcholine (nAChN) receptors are selectively activated by spinosyns and relatively insensitive to neonicotinoids.. RESULTS: The single-electrode voltage-clamp technique was used to measure the actions of newer nicotinic insecticides dinotefuran, sulfoxaflor, triflumezopyrim, spinetoram and GS-ω/k-hexatoxin-Hv1a on cockroach neuronal nAChD and nAChN currents.. Like imidacloprid and clothianidin, newer orthosteric nicotinic agonist insecticides dinotefuran and sulfoxaflor act by desensitizing nAChD receptors.



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    EPSCs after stimulation of the SCE and FCE.A, Type I fiber. Twin pulse stimulation of the SCE generating two EPSCs, the first by release of one transmitter quantum, the second of three quanta caused by facilitation.Asterisks in A and C mark spontaneously released single quanta. B, EPSCs after twin pulse stimulation of the FCE. The amplitude of the second EPSC is typically smaller than that of the first in type I fibers because of depression of release. C, Direct stimulation of a release bouton of FCE in a type IV fiber with a single pulse through the <t>macropatch</t> electrode. D, Stimulation and recording paradigm for the SCE and FCE. Both axons were stimulated selectively, as shown in A and B, but the FCE usually for a shorter period than the SCE. Only the EPSC amplitudes generated by the second of the twin pulses are plotted. In the experiment shown, 20 min was allowed for equilibration after 10−8m ω-AgaTX application before resuming stimulation and recording. The SCE was stimulated first. The short equilibration time was chosen to show the gradual development of the toxin effect.
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    EPSCs after stimulation of the SCE and FCE.A, Type I fiber. Twin pulse stimulation of the SCE generating two EPSCs, the first by release of one transmitter quantum, the second of three quanta caused by facilitation.Asterisks in A and C mark spontaneously released single quanta. B, EPSCs after twin pulse stimulation of the FCE. The amplitude of the second EPSC is typically smaller than that of the first in type I fibers because of depression of release. C, Direct stimulation of a release bouton of FCE in a type IV fiber with a single pulse through the <t>macropatch</t> electrode. D, Stimulation and recording paradigm for the SCE and FCE. Both axons were stimulated selectively, as shown in A and B, but the FCE usually for a shorter period than the SCE. Only the EPSC amplitudes generated by the second of the twin pulses are plotted. In the experiment shown, 20 min was allowed for equilibration after 10−8m ω-AgaTX application before resuming stimulation and recording. The SCE was stimulated first. The short equilibration time was chosen to show the gradual development of the toxin effect.
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    Hilgenberg gmbh macropatch pipettes
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    Makita macropatch
    EPSCs after stimulation of the SCE and FCE.A, Type I fiber. Twin pulse stimulation of the SCE generating two EPSCs, the first by release of one transmitter quantum, the second of three quanta caused by facilitation.Asterisks in A and C mark spontaneously released single quanta. B, EPSCs after twin pulse stimulation of the FCE. The amplitude of the second EPSC is typically smaller than that of the first in type I fibers because of depression of release. C, Direct stimulation of a release bouton of FCE in a type IV fiber with a single pulse through the <t>macropatch</t> electrode. D, Stimulation and recording paradigm for the SCE and FCE. Both axons were stimulated selectively, as shown in A and B, but the FCE usually for a shorter period than the SCE. Only the EPSC amplitudes generated by the second of the twin pulses are plotted. In the experiment shown, 20 min was allowed for equilibration after 10−8m ω-AgaTX application before resuming stimulation and recording. The SCE was stimulated first. The short equilibration time was chosen to show the gradual development of the toxin effect.
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    EPSCs after stimulation of the SCE and FCE.A, Type I fiber. Twin pulse stimulation of the SCE generating two EPSCs, the first by release of one transmitter quantum, the second of three quanta caused by facilitation.Asterisks in A and C mark spontaneously released single quanta. B, EPSCs after twin pulse stimulation of the FCE. The amplitude of the second EPSC is typically smaller than that of the first in type I fibers because of depression of release. C, Direct stimulation of a release bouton of FCE in a type IV fiber with a single pulse through the macropatch electrode. D, Stimulation and recording paradigm for the SCE and FCE. Both axons were stimulated selectively, as shown in A and B, but the FCE usually for a shorter period than the SCE. Only the EPSC amplitudes generated by the second of the twin pulses are plotted. In the experiment shown, 20 min was allowed for equilibration after 10−8m ω-AgaTX application before resuming stimulation and recording. The SCE was stimulated first. The short equilibration time was chosen to show the gradual development of the toxin effect.

    Journal: The Journal of Neuroscience

    Article Title: The Neuromuscular Junctions of the Slow and the Fast Excitatory Axon in the Closer of the Crab Eriphia spinifrons Are Endowed with Different Ca 2+ Channel Types and Allow Neuron-Specific Modulation of Transmitter Release by Two Neuropeptides

    doi: 10.1523/JNEUROSCI.22-03-00708.2002

    Figure Lengend Snippet: EPSCs after stimulation of the SCE and FCE.A, Type I fiber. Twin pulse stimulation of the SCE generating two EPSCs, the first by release of one transmitter quantum, the second of three quanta caused by facilitation.Asterisks in A and C mark spontaneously released single quanta. B, EPSCs after twin pulse stimulation of the FCE. The amplitude of the second EPSC is typically smaller than that of the first in type I fibers because of depression of release. C, Direct stimulation of a release bouton of FCE in a type IV fiber with a single pulse through the macropatch electrode. D, Stimulation and recording paradigm for the SCE and FCE. Both axons were stimulated selectively, as shown in A and B, but the FCE usually for a shorter period than the SCE. Only the EPSC amplitudes generated by the second of the twin pulses are plotted. In the experiment shown, 20 min was allowed for equilibration after 10−8m ω-AgaTX application before resuming stimulation and recording. The SCE was stimulated first. The short equilibration time was chosen to show the gradual development of the toxin effect.

    Article Snippet: The macropatch electrode is specific for current recording within the region of the electrode lumen with an amplifier designed for stimulating and recording from individual release sites (Zeitz Instruments, Augsburg, Germany).

    Techniques: Generated