lunascript rt master mix kit (New England Biolabs)
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lunascript rt master mix kit
Lunascript Rt Master Mix Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lunascript+rt+superscript+kit/LunaScript+RT+Master+Mix+(Primer-free)+Kit/pmc12386592-146-33-38
Average 97 stars, based on 138 article reviews
Lunascript Rt Master Mix Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lunascript+rt+superscript+kit/LunaScript+RT+Master+Mix+(Primer-free)+Kit/pmc12386592-146-33-38
Average 97 stars, based on 138 article reviews
lunascript rt master mix kit - by Bioz Stars,
2026-10
97/100 stars
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cDNA Synthesis:Article Title: Nuclear envelope budding enables export of large transcripts in muscle cells. Article Snippet: RNA was eluted in 50 μl of nuclease-free water and the concentration was determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific). .. First-strand cDNA synthesis was performed on all RNA samples using the other:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: Biotinylated isolectin GS-IB4 (GSL; Thermo Fisher, I21414) and streptavidin magnetic beads were used to further purify the brain vascular fragments. Isolation:Article Title: Hepatitis C Virus 5′UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA Synthesis Article Snippet: Reagents: Enzymes: T7 RNA polymerase (New England Biolabs (NEB), Frankfurt am Main, Germany; M0251L); DNase I (RNase free) (NEB M0303S); TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA; AM2238); Maxima Reverse Transcriptase (Thermo Fisher EP0743). .. Kits: Click iT Nascent RNA Capture Kit (Invitrogen, Life Technologies GmbH, Darmstadt, Germany; C10365 ); Monarch RNA isolation kit (NEB T2040L); Article Title: Virus-Like Particles: The Next Frontier in Livestock Gene Editing Article Snippet: .. Therefore, RNA of wildtype and VLP-transduced organoids was isolated, and cDNA was synthesized using the Article Title: Hepatitis C Virus 5'UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA Synthesis. Article Snippet: Reagents: Enzymes: T7 RNA polymerase (New England Biolabs (NEB), Frankfurt am Main, Germany; M0251L); DNase I (RNase free) (NEB M0303S); TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA; AM2238); Maxima Reverse Transcriptase (Thermo Fisher EP0743). .. Kits: Click iT Nascent RNA Capture Kit (Invitrogen, Life Technologies GmbH, Darmstadt, Germany; C10365); Monarch RNA isolation kit (NEB T2040L); Real-time Polymerase Chain Reaction:Article Title: Hepatitis C Virus 5′UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA Synthesis Article Snippet: Reagents: Enzymes: T7 RNA polymerase (New England Biolabs (NEB), Frankfurt am Main, Germany; M0251L); DNase I (RNase free) (NEB M0303S); TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA; AM2238); Maxima Reverse Transcriptase (Thermo Fisher EP0743). .. Kits: Click iT Nascent RNA Capture Kit (Invitrogen, Life Technologies GmbH, Darmstadt, Germany; C10365 ); Monarch RNA isolation kit (NEB T2040L); Article Title: ER-associated control of axonal vesicle trafficking during neuronal development Article Snippet: RNA quantity and purity were assessed by spectrophotometry (NanoDrop, Thermo Fisher Scientific). .. For each sample, 500 ng to 1 μg of RNA was reverse-transcribed using the Article Title: Hepatitis C Virus 5'UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA Synthesis. Article Snippet: Reagents: Enzymes: T7 RNA polymerase (New England Biolabs (NEB), Frankfurt am Main, Germany; M0251L); DNase I (RNase free) (NEB M0303S); TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA; AM2238); Maxima Reverse Transcriptase (Thermo Fisher EP0743). .. Kits: Click iT Nascent RNA Capture Kit (Invitrogen, Life Technologies GmbH, Darmstadt, Germany; C10365); Monarch RNA isolation kit (NEB T2040L); Luciferase:Article Title: Hepatitis C Virus 5′UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA Synthesis Article Snippet: Reagents: Enzymes: T7 RNA polymerase (New England Biolabs (NEB), Frankfurt am Main, Germany; M0251L); DNase I (RNase free) (NEB M0303S); TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA; AM2238); Maxima Reverse Transcriptase (Thermo Fisher EP0743). .. Kits: Click iT Nascent RNA Capture Kit (Invitrogen, Life Technologies GmbH, Darmstadt, Germany; C10365 ); Monarch RNA isolation kit (NEB T2040L); Article Title: Hepatitis C Virus 5'UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA Synthesis. Article Snippet: Reagents: Enzymes: T7 RNA polymerase (New England Biolabs (NEB), Frankfurt am Main, Germany; M0251L); DNase I (RNase free) (NEB M0303S); TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA; AM2238); Maxima Reverse Transcriptase (Thermo Fisher EP0743). .. Kits: Click iT Nascent RNA Capture Kit (Invitrogen, Life Technologies GmbH, Darmstadt, Germany; C10365); Monarch RNA isolation kit (NEB T2040L); Staining:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # Membrane:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # Gel Extraction:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # DNA Purification:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # Chromatin Immunoprecipitation:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # DNA Sequencing:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # Multiplex Assay:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # Recombinant:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # Plasmid Preparation:Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit mAb anti-LEF1 Cell Signaling Technology Cat. # 2230 rabbit mAb anti-ERG Cell Signaling Technology Cat. # 97249 chicken polyclonal anti-GFP Aves Labs Cat. # GFP-1020 rat monoclonal anti-PECAM1 BD Biosciences Cat. # 553370 goat polyclonal anti-mScarlet Biorbyt Cat. # orb1463262 rat monoclonal anti-PLVAP BD Biosciences Cat. # 553849 rabbit monoclonal anti-GLUT1 Invitrogen Cat. # MA5-31960 mouse monoclonal anti-α Smooth Muscle Actin, Alexa Fluor 647 conjugated BioLegend Cat. # 614855 rabbit monoclonal anti-GLUT1, iFluor 488 conjugated HUABIO Cat. # HA720154F rabbit monoclonal anti-ERG, Alexa Fluor 647 conjugated Abcam Cat. # ab196149 Rabbit mAb IgG XP Isotype Control Cell Signaling Technology Cat. # 66362 Alexa dye-conjugated secondary antibodies (multiple) Thermo Fisher N/A Bacterial and virus strains NEB-Stable E. coli New England BioLabs Cat# C3040H Chemicals, peptides, and recombinant proteins Cas9 protein Integrated DNA Technologies (IDT) Cat. # 1081058 NucleoBond Xtra Midi Plus EF kit TaKaRa Cat. # 740422.10 Isoflurane MP Biomedicals Cat. # ICN15195790 1 ml tuberculin syringe Becton, Dickinson Cat. # BD 309659 30 gauge x 1/2 inch PrecisionGlide Needle Becton, Dickinson Cat. # BD 305106 Trypan blue Gibco Cat. #15250061 EasyTouch Insulin Syringe (30G 1cc 5/16") Amazon N/A SeaPlaque Agarose Lonza Cat. # BMA50100 Fluoromount G EM Sciences Cat. # 17984–25 Dulbecco’s Phosphate Buffered Saline (no Ca or Mg) Gibco Cat. # 14190144 EDTA Thermo Fisher Cat. # AM9262 RNasin-Plus RNase Inhibitor Promega Cat. # N2615 Biotinylated isolectin GS-IB4 (GSL) Thermo Fisher cat. # I21414 Isolectin GS-IB4 from Griffonia simplicifolia (GSL), Alexa Fluor 488 conjugated Thermo Fisher Cat. # I21411 Ficoll PM 400 Sigma-Aldrich Cat. # F4375 Streptavidin Magnetic Beads Thermo Fisher Cat. # 88816 Spermidine Sigma Cat. # S2501 1x cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat. # 11873580001 300 μm Nylon Mesh Filters, 47 mm diameter, Nonsterile Tisch Scientific Cat. # ME17240 20 μm filter (pluriStrainer) Cell Strainer Cat # 43-50020-03 Direct-zol RNA Microprep Kits Zymo Cat. # R2061 TRI reagent Zymo Cat. # R2050-1-50 (Continued on next page) e1 Neuron 114, 1–15.e1–e9, May 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # Reverse Transcription:Article Title: ER-associated control of axonal vesicle trafficking during neuronal development Article Snippet: RNA quantity and purity were assessed by spectrophotometry (NanoDrop, Thermo Fisher Scientific). .. For each sample, 500 ng to 1 μg of RNA was reverse-transcribed using the Synthesized:Article Title: Virus-Like Particles: The Next Frontier in Livestock Gene Editing Article Snippet: .. Therefore, RNA of wildtype and VLP-transduced organoids was isolated, and cDNA was synthesized using the Amplification:Article Title: Virus-Like Particles: The Next Frontier in Livestock Gene Editing Article Snippet: .. Therefore, RNA of wildtype and VLP-transduced organoids was isolated, and cDNA was synthesized using the Polymerase Chain Reaction:Article Title: Virus-Like Particles: The Next Frontier in Livestock Gene Editing Article Snippet: .. Therefore, RNA of wildtype and VLP-transduced organoids was isolated, and cDNA was synthesized using the |