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superscript iii reverse transcription kit  (New England Biolabs)


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    Structured Review

    New England Biolabs superscript iii reverse transcription kit
    Superscript Iii Reverse Transcription Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lunascript+rt+superscript+kit/LunaScript+RT+SuperMix/pmc07356975-91-22-34
    Average 97 stars, based on 1249 article reviews
    superscript iii reverse transcription kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: A two-component RUBY reporter system to assess plant virus infection.
    Article Snippet: .. Total RNA isolated from cDNA derived infections or subsequent mechanical passages were reverse-transcribed using LunaScript RT SuperMix (NEB, Ipswich, MA USA) and first strand-synthesis used for subsequent PCR analysis. ..

    Derivative Assay:

    Article Title: A two-component RUBY reporter system to assess plant virus infection.
    Article Snippet: .. Total RNA isolated from cDNA derived infections or subsequent mechanical passages were reverse-transcribed using LunaScript RT SuperMix (NEB, Ipswich, MA USA) and first strand-synthesis used for subsequent PCR analysis. ..

    Reverse Transcription:

    Article Title: A two-component RUBY reporter system to assess plant virus infection.
    Article Snippet: .. Total RNA isolated from cDNA derived infections or subsequent mechanical passages were reverse-transcribed using LunaScript RT SuperMix (NEB, Ipswich, MA USA) and first strand-synthesis used for subsequent PCR analysis. ..

    Article Title: Phosphorylation of YBX1 in the Kidneys is Altered in Legumain Knockout-Mice.
    Article Snippet: The isolated RNA concentration was determined by using a NanoDrop One/One spectrophotometer (Thermo Scientific). .. In the reverse transcription reaction, 1 mg of total RNA was used with LunaScript RT Supermix (New England Biolabs). .. For the RT-qPCR reaction, 10 ng of cDNA per well was used with KAPA SYBR FAST (Merck) master mix.

    Article Title: PARP7 inhibition and a STING agonist potentiate radiation-induced immunogenicity in glioblastoma
    Article Snippet: The VBCF NGS facility performed sequencing on a NovaSeq X sequencing system (Illumina) in read mode SR100. .. 1 μg of RNA was reverse transcribed using LunaScript RT SuperMix (NEB). cDNA was diluted 1:5 in H 2 O. qPCR was performed on a Bio-Rad CFX Opus 96 cycler operated by Bio-Rad CFX Maestro software (Version 2.3) using iQTM Multiplex Powermix (Bio-Rad). .. IFNB1 , CXCL10 , PD-L1 ( CD274 ), IL-6 ( IL6 ), TNFα (TNF ) and TBP levels were quantified by multiplexing RT-PCR using the commercially available primers (human IFNB1 UniqueAssayID: qHsaCEP0054112, human CXCL10 UniqueAssayID: qHsaCEP0053880, human IL6 UniqueAssayID: qHsaCEP0051939, human TBP UniqueAssayID: qHsaCIP0036255, human CD274 UniqueAssayID: qHsaCIP0039192, human TNF UniqueAssayID: qHsaCEP0040184). qPCR data were analyzed and plotted using GraphPad Prism (9.1.1).

    Article Title: SARS-CoV-2 Introductions into Lao PDR Revealed by Genomic Surveillance, 2021–2024
    Article Snippet: .. Briefly, viral RNA was reverse-transcribed using LunaScript RT SuperMix (New England Biolabs) and SARS-CoV-2 amplicons were obtained using the Q5 HS Master Mix (New England Biolabs). .. Samples were multiplexed using Oxford Nanopore rapid barcode kit (SQK-RBK110.96) and run in batches of 4-24 samples on R9 flow cells using the MinION Mk1B sequencer.

    Polymerase Chain Reaction:

    Article Title: A two-component RUBY reporter system to assess plant virus infection.
    Article Snippet: .. Total RNA isolated from cDNA derived infections or subsequent mechanical passages were reverse-transcribed using LunaScript RT SuperMix (NEB, Ipswich, MA USA) and first strand-synthesis used for subsequent PCR analysis. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Scaling Multiplex qPCR Primer Design to 1000-plex using the Degenerate Incomplete Multiplex Primer List Extension (DIMPLE) Algorithm
    Article Snippet: Bisulfite conversion was performed using the EZ DNA Methylation-Lightning Kit (Zymo Research) with 1–10 ng input DNA. .. For fusion detection assays, cDNA served as the qPCR template. cDNA was synthesized from up to 1000 ng total RNA using LunaScript RT SuperMix (New England Biolabs): 25°Cx2 min, 55°Cx10 min, 95°Cx1 min. Products were purified with 1.8× Ampure XP beads (Beckman Coulter, Brea, CA). ..

    Article Title: Small RNA genomics of Aedes aegypti mosquitoes discovers infectious viruses that trigger an RNA interference response
    Article Snippet: For RT-PCR, 1 μg RNA was used for first-strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit followed by Phusion High-Fidelity DNA Polymerase (NEB). .. RT-qPCR was performed with LunaScript RT Supermix (NEB) in a Bio-Rad CFX Opus 96 Real-Time PCR System. ..

    Article Title: PARP7 inhibition and a STING agonist potentiate radiation-induced immunogenicity in glioblastoma
    Article Snippet: The VBCF NGS facility performed sequencing on a NovaSeq X sequencing system (Illumina) in read mode SR100. .. 1 μg of RNA was reverse transcribed using LunaScript RT SuperMix (NEB). cDNA was diluted 1:5 in H 2 O. qPCR was performed on a Bio-Rad CFX Opus 96 cycler operated by Bio-Rad CFX Maestro software (Version 2.3) using iQTM Multiplex Powermix (Bio-Rad). .. IFNB1 , CXCL10 , PD-L1 ( CD274 ), IL-6 ( IL6 ), TNFα (TNF ) and TBP levels were quantified by multiplexing RT-PCR using the commercially available primers (human IFNB1 UniqueAssayID: qHsaCEP0054112, human CXCL10 UniqueAssayID: qHsaCEP0053880, human IL6 UniqueAssayID: qHsaCEP0051939, human TBP UniqueAssayID: qHsaCIP0036255, human CD274 UniqueAssayID: qHsaCIP0039192, human TNF UniqueAssayID: qHsaCEP0040184). qPCR data were analyzed and plotted using GraphPad Prism (9.1.1).

    Article Title: Small RNA genomics of Aedes aegypti mosquitoes discovers infectious viruses that trigger an RNA interference response.
    Article Snippet: For RT-PCR, 1 μg RNA was used for first-strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit followed by Phusion High-Fidelity DNA Polymerase (NEB). .. RT-qPCR was performed with LunaScript RT Supermix (NEB) in a Bio-Rad CFX Opus 96 Real-Time PCR System. ..

    Synthesized:

    Article Title: Scaling Multiplex qPCR Primer Design to 1000-plex using the Degenerate Incomplete Multiplex Primer List Extension (DIMPLE) Algorithm
    Article Snippet: Bisulfite conversion was performed using the EZ DNA Methylation-Lightning Kit (Zymo Research) with 1–10 ng input DNA. .. For fusion detection assays, cDNA served as the qPCR template. cDNA was synthesized from up to 1000 ng total RNA using LunaScript RT SuperMix (New England Biolabs): 25°Cx2 min, 55°Cx10 min, 95°Cx1 min. Products were purified with 1.8× Ampure XP beads (Beckman Coulter, Brea, CA). ..

    Purification:

    Article Title: Scaling Multiplex qPCR Primer Design to 1000-plex using the Degenerate Incomplete Multiplex Primer List Extension (DIMPLE) Algorithm
    Article Snippet: Bisulfite conversion was performed using the EZ DNA Methylation-Lightning Kit (Zymo Research) with 1–10 ng input DNA. .. For fusion detection assays, cDNA served as the qPCR template. cDNA was synthesized from up to 1000 ng total RNA using LunaScript RT SuperMix (New England Biolabs): 25°Cx2 min, 55°Cx10 min, 95°Cx1 min. Products were purified with 1.8× Ampure XP beads (Beckman Coulter, Brea, CA). ..

    Quantitative RT-PCR:

    Article Title: Small RNA genomics of Aedes aegypti mosquitoes discovers infectious viruses that trigger an RNA interference response
    Article Snippet: For RT-PCR, 1 μg RNA was used for first-strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit followed by Phusion High-Fidelity DNA Polymerase (NEB). .. RT-qPCR was performed with LunaScript RT Supermix (NEB) in a Bio-Rad CFX Opus 96 Real-Time PCR System. ..

    Article Title: Small RNA genomics of Aedes aegypti mosquitoes discovers infectious viruses that trigger an RNA interference response.
    Article Snippet: For RT-PCR, 1 μg RNA was used for first-strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit followed by Phusion High-Fidelity DNA Polymerase (NEB). .. RT-qPCR was performed with LunaScript RT Supermix (NEB) in a Bio-Rad CFX Opus 96 Real-Time PCR System. ..

    Maestro Software:

    Article Title: PARP7 inhibition and a STING agonist potentiate radiation-induced immunogenicity in glioblastoma
    Article Snippet: The VBCF NGS facility performed sequencing on a NovaSeq X sequencing system (Illumina) in read mode SR100. .. 1 μg of RNA was reverse transcribed using LunaScript RT SuperMix (NEB). cDNA was diluted 1:5 in H 2 O. qPCR was performed on a Bio-Rad CFX Opus 96 cycler operated by Bio-Rad CFX Maestro software (Version 2.3) using iQTM Multiplex Powermix (Bio-Rad). .. IFNB1 , CXCL10 , PD-L1 ( CD274 ), IL-6 ( IL6 ), TNFα (TNF ) and TBP levels were quantified by multiplexing RT-PCR using the commercially available primers (human IFNB1 UniqueAssayID: qHsaCEP0054112, human CXCL10 UniqueAssayID: qHsaCEP0053880, human IL6 UniqueAssayID: qHsaCEP0051939, human TBP UniqueAssayID: qHsaCIP0036255, human CD274 UniqueAssayID: qHsaCIP0039192, human TNF UniqueAssayID: qHsaCEP0040184). qPCR data were analyzed and plotted using GraphPad Prism (9.1.1).

    Multiplex Assay:

    Article Title: PARP7 inhibition and a STING agonist potentiate radiation-induced immunogenicity in glioblastoma
    Article Snippet: The VBCF NGS facility performed sequencing on a NovaSeq X sequencing system (Illumina) in read mode SR100. .. 1 μg of RNA was reverse transcribed using LunaScript RT SuperMix (NEB). cDNA was diluted 1:5 in H 2 O. qPCR was performed on a Bio-Rad CFX Opus 96 cycler operated by Bio-Rad CFX Maestro software (Version 2.3) using iQTM Multiplex Powermix (Bio-Rad). .. IFNB1 , CXCL10 , PD-L1 ( CD274 ), IL-6 ( IL6 ), TNFα (TNF ) and TBP levels were quantified by multiplexing RT-PCR using the commercially available primers (human IFNB1 UniqueAssayID: qHsaCEP0054112, human CXCL10 UniqueAssayID: qHsaCEP0053880, human IL6 UniqueAssayID: qHsaCEP0051939, human TBP UniqueAssayID: qHsaCIP0036255, human CD274 UniqueAssayID: qHsaCIP0039192, human TNF UniqueAssayID: qHsaCEP0040184). qPCR data were analyzed and plotted using GraphPad Prism (9.1.1).

    cDNA Synthesis:

    Article Title: Profiles of stress hormones in relation to DENV serotypes among dengue-positive patients
    Article Snippet: Viral RNA was extracted from selected patient samples using the QIAamp Viral RNA Mini Kit (Qiagen, USA). .. RNA quantity and quality were assessed using a Nanodrop plate reader (SkanIt RE 4.1, Thermo Scientific, USA). cDNA synthesis was performed using the LunaScript® RT SuperMix (New England Biolabs) and RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher, USA) as per the manufacturer’s protocols. cDNA samples were shipped to Theragen (Korea) for total mRNA sequencing using the Illumina NovaSeq 6000 platform with a 150 PE sequencing run. .. Duplicate samples were used for whole-genome amplicon sequencing at the Korea Research Institute for Standards and Science (KRISS), using the Oxford Nanopore GridION platform.

    Sequencing:

    Article Title: Profiles of stress hormones in relation to DENV serotypes among dengue-positive patients
    Article Snippet: Viral RNA was extracted from selected patient samples using the QIAamp Viral RNA Mini Kit (Qiagen, USA). .. RNA quantity and quality were assessed using a Nanodrop plate reader (SkanIt RE 4.1, Thermo Scientific, USA). cDNA synthesis was performed using the LunaScript® RT SuperMix (New England Biolabs) and RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher, USA) as per the manufacturer’s protocols. cDNA samples were shipped to Theragen (Korea) for total mRNA sequencing using the Illumina NovaSeq 6000 platform with a 150 PE sequencing run. .. Duplicate samples were used for whole-genome amplicon sequencing at the Korea Research Institute for Standards and Science (KRISS), using the Oxford Nanopore GridION platform.



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