dna polymerases (New England Biolabs)

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New England Biolabs dna polymerases
Dna Polymerases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lunascript+rt+superscript+kit/DNA+Polymerase+I/pmc11635996-88-47-63
Average 99 stars, based on 1549 article reviews

dna polymerases - by Bioz Stars, 2026-09

99/100 stars

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Virus:

Article Title: HIV-driven virome dysbiosis unveils distinct virome features and inter-viral correlations in blood and respiratory niches.
Article Snippet: To selectively concentrate viral particles, we performed enzymatic digestion using a nuclease cocktail (TURBO DNase from Ambion with Cat.NO.AM2239, Benzonase Nuclease from Merck with Cat.NO.70664-3, Baseline-ZERO from Epicentre with Cat.NO.DB0715K and RNase A from Thermo Fisher Scientific with Cat.NO.EN0531), 34μl/pool at 37°C for 60 minutes, effectively eliminating unprotected host and environmental nucleic acids 74. .. Viral nucleic acids were extracted using the QIAamp MinElute Virus Spin Kit. cDNA synthesis used SuperScript III (Invitrogen, 18080093), 1μl/pool and Klenow polymerase (NEB, M0210L), 1μl/pool followed by library preparation With Nextera XT Pre kit (Illumina, FC-131-1024) and paired-end sequencing (MiSeq, 2×250 bp). ..

cDNA Synthesis:

Sequencing:

Sonication:

Article Title: Lamins and lineage-relevant transcription factors coordinate gene expression in lineage development
Article Snippet: .. End repair of the sonicated DNA was performed by mixing 50 μL of sonicated Hi-C DNA with 10 μL of 10x phosphorylation reaction buffer (NEB, B0201S), 0.5 μL of T4 DNA polymerase (NEB, M0203S), 0.5 μL of T4 polynucleotide kinase (NEB, M0201S), 5 μL of 10 mM dNTP mix (Qiagen, 201912), 15 μL of 10 mM dATP (NEB, N0440S), 2 μL of 10x diluted Klenow fragment DNA polymerase I (NEB, M0210L), and 17 μL of distilled water. ..

Hi-C:

Phospho-proteomics:

Polymerase Chain Reaction:

Article Title: Methods for indexing samples and sequencing multiple polynucleotide templates
Article Snippet: The recovered volumes were each purified with a Qiagen PCR purification kit column and eluted in 30 μl of EB. .. Step 2) End-Repair Materials: Nebulized DNA (from Step 1) Water T4 DNA ligase buffer with 10 mM ATP (10×) (NEB, B0202S) dNTPs mix (10 mM each) (NEB, N0447S) T4 DNA Polymerase (3 U/ul) (NEB, M0203L) E. coli DNA Pol I large fragment (Klenow) (5 U/ul) (NEB, M0210S) T4 polynucleotide kinase (10 U/ul) (NEB, M0201L) PCR purification kit columns (Qiagen, 28104) Procedure: End repair mix was assembled as follows: Nebulized DNA 30 μl Water 45 μl T4 DNA ligase buffer with 10 mM ATP 10 μl dNTPs 4 μl T4 DNA pol 5 μl Klenow DNA pol 1 ul T4 PNK 5 ul 100 μl total The reaction was incubated for 30 minutes at room temperature. .. The DNA was purified on a Qiagen column, eluting in 30 μl EB.

Purification:

Incubation:

Article Title: Identification of Parkinson’s disease-associated regulatory variants in human dopaminergic neurons reveals modulators of SCARB2 and BAG3 expression
Article Snippet: .. To fill the restriction fragment overhangs, 18.75 μl of 0.4mM biotin-14-dCTP (19518018, ThermoFisher), 0.75 μl of 10 mM dATP (U120D, Promega), 0.75 μl of 10 mM dGTP (U121D, Promega), 0.75 μl of 10 mM dTTP (U123D, Promega) and 8 μl of 5U/μl DNA polymerase I Klenow (NEB M0210L, New England Biolabs) was added and the samples were incubated at 37°C for 90 minutes in a thermomixer at 400 rpm. .. The step of DNA ligation was set up by adding 657 μl of nuclease-free water, 120 μl of 10X T4 DNA Ligase buffer (EL0011, ThermoFisher), 100 μl of 10% Triton X-100 (Sigma-Aldrich, X100), 12 μl of 20 mg/ml bovine serum albumin (BSA) (Carl Roth, 8076.4) and 5 μl of 5 Weiss U/μl T4 DNA ligase (EL0011, ThermoFisher) to the samples that were incubated at room temperature for 4 hours on a rotating wheel.

Article Title: Stage-specific epigenetic priming amplifies gene activation during lineage commitment
Article Snippet: First, for addition of 5′-phosphate groups and removal of 3′-phosphate groups, digested samples were incubated with 10xNEBuffer 2.1 (NEB, #B7202S), 100 mM ATP (NEB, #P0756), 100 mM DTT, and T4 PNK (10 U/μl; NEB, #M0201), at 37°C for 15 min with interval mixing. .. Then, 50 U of the DNA Polymerase I Klenow Fragment (NEB, #M0210) was added to the reaction and incubated at 37°C for 15 min with interval mixing. .. Next, the following mixture was added to each sample: 10 mM deoxyguanosine triphosphate (dGTP) and deoxythymidine triphosphate (dTTP) (NEB, #N0446), 1 mM biotin-dATP, and biotin–deoxycytidine triphosphate (dCTP) (Jena Bioscience, #NU-835-BIO14 and #NU-809-BIOX), T4 DNA ligase buffer, and BSA.

Plasmid Preparation:

Article Title: Distinct mechanisms of replication stress induced by oncogenic RAS and cyclin E1 converge on R-loop-dependent fork reversal.
Article Snippet: .. The cleaved vector was religated by T4 DNA ligase (NEB, M0202S) after filling in DNA ends with Klenow Fragment (NEB, M0210S). pBABEneo-CCNE1 was constructed by inserting the Bsp1407I/HindIII fragment of pLXSNneo-CCNE1 (kindly provided by Dr. Stefano Ferrari) including the CCNE1 gene between the Bsp1407I and HindIII sites in pBABEneo. ..

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