read sequencing (Plasmidsaurus)

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Plasmidsaurus read sequencing
Read Sequencing, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/long-read+sequencing/Custom+Sequencing/pm42881158-336-5-9
Average 97 stars, based on 1 article reviews

read sequencing - by Bioz Stars, 2026-11

97/100 stars

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Article Title: Leveraging high temperature for enhanced and faster detection of SARS-CoV-2 and its Omicron variant with CRISPR-AsCas12a.
Article Snippet: CRISPR-based methods are effective for detecting SARS-CoV-2, with performance comparable to gold-standard PCR.. Developing simpler, faster, and more sensitive CRISPR-based diagnostics will accelerate their adoption in field.. Adjusting the temperature at which Cas proteins operate is crucial for enhancing DNA recognition kinetics, thereby improving specificity, sensitivity, and practicality.

Article Title: Vangl2 acts in distinct cell types to establish bidirectional hair-bundle polarity and maintains tissue-wide alignment in zebrafish neuromasts
Article Snippet: The amplicon was gel extracted and cloned into the pDONR221 vector.

Article Title: Reciprocal, methylation-dependent binding of Zfp57 and Gzf1 safeguards Dlk1-Dio3 imprinting during developmental reprogramming
Article Snippet: Primary antibody against H3K4me3 (cellsignal #9751) was added at a 1:50 dilution and incubated overnight at 4 °C.

Plasmid Preparation:

Article Title: Mobile genetic elements drive a plasmid fusion and deletion lifecycle shaping evolution and antimicrobial resistance.
Article Snippet: .. Plasmid architectures were determined by long-read sequencing performed by Plasmidsaurus (San Francisco, CA, USA) using Oxford Nanopore Technologies. ..

Sequencing:

Article Title: A human transcriptomic deletion links cortical expansion and cancer
Article Snippet: .. We verified all plasmids with long read sequencing before use (Plasmidosaurus). .. NIH/3T3 cells (cat no. CRL-1658) were obtained from American Type Culture Collection (Manassas, VA).

Article Title: Genome shuffling enables quantitative trait locus mapping in Bacillus subtilis
Article Snippet: .. The colonies that failed to grow on these plates were analyzed for the presence of the desired edits using PCR amplification of the targeted region, followed by long-read sequencing (Plasmidsaurus Inc., Eugene, OR, USA). .. Select strains were also verified by whole-genome sequencing on an Illumina MiSeq instrument (Illumina, San Diego, CA) as described previously .

Article Title: From junk to deleterious: Natural subtelomeric repeat amplifications impact fitness and cellular phenotypes in yeast
Article Snippet: .. All plasmids were validated by long-read sequencing (Plasmidsaurus). ..

Polymerase Chain Reaction:

Amplification:

Long-read Sequencing:

Article Title: Chromosomal integration of a bcrRABD-carrying integrative and conjugative element increases bacitracin resistance in Clostridium perfringens following restrictions in antimicrobial use in Canadian broiler chickens
Article Snippet: After adjusting all samples at 50 ng/μL in a final volume of 20 μL, samples were sent at the Genome Quebec Centre (Montreal, Quebec, Canada) sequencing centre and sequenced on a NovaSeq6000 SP instrument (Illumina) to generate 150 bp paired-end reads. .. From the selected isolates, the total DNA of two isolates (isolates #24 and #103) exhibiting high resistance to bacitracin (>256 μg/mL) was also send to Plasmidsaurus Inc. for long-read sequencing using the Oxford Nanopore Technology (ONT). ..

Review