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lipofectin ® method  (Thermo Fisher)


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    Structured Review

    Thermo Fisher lipofectin ® method
    Lipofectin ® Method, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipofectin+method/pm24960080-94-5-8
    Average 90 stars, based on 1 article reviews
    lipofectin ® method - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat.
    Article Snippet: .. The cells were washed with DMEM twice and incubated with 2 ml DMEM before transfection with 10 μg of plasmid DNA by Lipofectin method (Life Technology, GIBCO BRL). ..

    Transfection:

    Article Title: Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat.
    Article Snippet: .. The cells were washed with DMEM twice and incubated with 2 ml DMEM before transfection with 10 μg of plasmid DNA by Lipofectin method (Life Technology, GIBCO BRL). ..

    Article Title: Conditional expression of PTEN alters the androgen responsiveness of prostate cancer cells.
    Article Snippet: Z. Wu, M. Conaway, D. Gioeli, M.J. Weber, and D. Theodorescu* DepartmentofMolecular PhysiologyandBiological Physics,Universityof VirginiaHealth Sciences Center, Charlottesville,Virginia DepartmentofHealth Evaluation Sciences (Biostatistics),Universityof VirginiaHealth SciencesCenter, Charlottesville,Virginia DepartmentofMicrobiology,Universityof VirginiaHealth Sciences Center,Charlottesville,Virginia

    Article Title: Differential Ras activation between caveolae/raft and non-raft microdomains.
    Article Snippet: .. Cell culture, transfection, and imaging COS cells were transfected with pRaichu-tH or pRaichu-tK using the Lipofectin method (Gibco BRL). .. For disruption of caveolae/ rafts, cells were incubated in Hank’s Balanced Salt Solution (HBSS) containing 10 mM methyl-β-cyclodextrin (MβCD) (Sigma-Aldrich) for 30 min at 37°C (Prior et al., 2003; Roy et al., 1999).

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen).

    Article Title: A novel point mutation in the ligand-binding domain (LBD) of the human glucocorticoid receptor (hGR) causing generalized glucocorticoid resistance: the importance of the C terminus of hGR LBD in conferring transactivational activity.
    Article Snippet: .. CV-1, COS-7, and HCT-116 cells were transfected using the lipofectin method (Invitrogen Life Technologies, Inc., Gaithersburg, MD) as previously described (26). .. HeLa cells were transfected using FuGene 6 reagent according to the instructions of the manufacturer (Roche, Indianapolis, IN).

    Plasmid Preparation:

    Article Title: Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat.
    Article Snippet: .. The cells were washed with DMEM twice and incubated with 2 ml DMEM before transfection with 10 μg of plasmid DNA by Lipofectin method (Life Technology, GIBCO BRL). ..

    Cell Culture:

    Article Title: Differential Ras activation between caveolae/raft and non-raft microdomains.
    Article Snippet: .. Cell culture, transfection, and imaging COS cells were transfected with pRaichu-tH or pRaichu-tK using the Lipofectin method (Gibco BRL). .. For disruption of caveolae/ rafts, cells were incubated in Hank’s Balanced Salt Solution (HBSS) containing 10 mM methyl-β-cyclodextrin (MβCD) (Sigma-Aldrich) for 30 min at 37°C (Prior et al., 2003; Roy et al., 1999).

    Imaging:

    Article Title: Differential Ras activation between caveolae/raft and non-raft microdomains.
    Article Snippet: .. Cell culture, transfection, and imaging COS cells were transfected with pRaichu-tH or pRaichu-tK using the Lipofectin method (Gibco BRL). .. For disruption of caveolae/ rafts, cells were incubated in Hank’s Balanced Salt Solution (HBSS) containing 10 mM methyl-β-cyclodextrin (MβCD) (Sigma-Aldrich) for 30 min at 37°C (Prior et al., 2003; Roy et al., 1999).

    Modification:

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen).

    Construct:

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen).

    Article Title: Tat-dependent repression of human immunodeficiency virus type 1 long terminal repeat promoter activity by fusion of cellular transcription factors.
    Article Snippet: Transcription initiation from HIV-1 long terminal repeat (LTR) promoter requires the virally encoded transactivator, Tat, and several cellular co-factors to accomplish the Tat-dependent processive transcription elongation.. Individual cellular transcription activators, LBP-1b and Oct-1, on the other hand, have been shown to inhibit LTR promoter activities probably via competitive binding against TFIID to the TATA-box in LTR promoter.. To explore the genetic interference strategies against the viral replication, we took advantage of the existence of the bipartite DNA binding domains and the repression domains of LBP-1b and Oct-1 factors to generate a chimeric transcription repressor.

    Luciferase:

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen).

    Expressing:

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen).

    Article Title: Tat-dependent repression of human immunodeficiency virus type 1 long terminal repeat promoter activity by fusion of cellular transcription factors.
    Article Snippet: Transcription initiation from HIV-1 long terminal repeat (LTR) promoter requires the virally encoded transactivator, Tat, and several cellular co-factors to accomplish the Tat-dependent processive transcription elongation.. Individual cellular transcription activators, LBP-1b and Oct-1, on the other hand, have been shown to inhibit LTR promoter activities probably via competitive binding against TFIID to the TATA-box in LTR promoter.. To explore the genetic interference strategies against the viral replication, we took advantage of the existence of the bipartite DNA binding domains and the repression domains of LBP-1b and Oct-1 factors to generate a chimeric transcription repressor.

    other:




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