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kapatm library quantification kit  (Roche)


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    Structured Review

    Roche kapatm library quantification kit
    Kapatm Library Quantification Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/library+quantification+kit/KAPA+Quantification+Kit/pmc13109727-77-9-13
    Average 99 stars, based on 5 article reviews
    kapatm library quantification kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Library Quantification:

    Article Title: Effect of Zearalenone on Microbial Spatial Differences in the Chyme and Intestinal Mucosa of Piglets.
    Article Snippet: .. Library size distribution was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and quantification was performed using the Kapa Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). .. Paired-end sequencing (2250 bp) was conducted on an Illumina NovaSeq 6000 platform (LC-Bio, Hangzhou, Zhejiang, China).

    Article Title: Exploring CSF microRNA signatures as diagnostic biomarkers in adult-type diffuse gliomas
    Article Snippet: .. Pooled libraries were quantified by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems). .. The Illumina NextSeq 500 was used to sequence the pooled library, one pool per run, in a single-end configuration, with a read length of 75 bp.

    Article Title: HDAC Inhibition Induces Transient Phenotypic Inertia in Dormant OCCC Spheroids by Derepression of Cell Cycle Genes.
    Article Snippet: Briefly, mRNA enrichment and library construction were performed using the Illumina Stranded mRNA Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s recommendations. .. Libraries were quantified using the KAPA Library Quantification Kits— Complete kit (Universal) (Kapa Biosystems, Wilmington, MA, USA), and the average fragment size was determined using Fragment Analyzer 5300 (Agilent, Santa Clara, CA, USA). ..

    Article Title: Whole-exome sequencing unveils novel potential gene mutations involved in primary renal small cell carcinoma
    Article Snippet: .. The concentration was accurately measured using the Qubit ® dsDNA HS Assay Kit (Thermo Fisher Scientific) and confirmed by quantitative PCR (qPCR) using the KAPA Library Quantification Kit (Roche, Catalog No. KK4824) to ensure precise molarity calculation for cluster generation. ..

    Article Title: Small regulatory RNAs modulate lactococcal susceptibility to cell wall-targeting antimicrobials
    Article Snippet: After ethanol precipitation, RNA was treated with RNA 5′ pyrophosphohydrolase (RppH) (New England Biolabs), recovered with RNA Clean & Concentrator-5 Kit, and 165 ng were taken for further processing using NEXTflex Small RNA-Seq Kit v3 (PerkinElmer) with the following modifications: at Step E, incubation at 42°C was extended to 60 min; at Step G, 16 polymerase chain reaction (PCR) cycles were performed; and Steps F and H1 were carried out as described in the alternative protocol for Preparing Libraries without Size Selection. .. The resulting complementary DNA (cDNA) libraries were quantified by KAPATM Library Quantification Kit (Roche) and sequenced on the Illumina MiSeqTM instrument using MiSeqTM Reagent Kit v2 chemistry (Illumina) for paired-end sequencing with 2 × 151 bp reads. ..

    Article Title: Cell culture methods and compositions
    Article Snippet: To remove excess primers and adaptor dimers, PCR products were purified twice using SPRI beads (Beckman Coulter). .. The quality of the libraries was assessed using Agilent Bioanalyzer DNA 1000 chip (Duke-NUS Genome Facility) and the molar concentration of each library was determined using KAPA library quantification kit (Roche). .. Three pooled libraries (each containing 9 samples with the pooled concentration of 8 nM in 30 uL) was sent for sequencing on Illumina HiSeq4000 (NovogeneAIT Genomics Singapore).

    Article Title: A Very-low-energy Fast Involves Increased Adipose Inflammatory Gene Expression: A 6-day Feeding Trial (FASTOMICS-6)
    Article Snippet: .. The final cDNA libraries were additionally quantified with the KAPA Library Quantification Kit (Roche, USA) for Illumina sequencing platforms. .. All samples were paired-end sequenced (2 × 100 bp) on the Illumina NovaSeq 6000 system (Illumina, San Diego, CA, USA).

    Article Title: Hypoxic CAF studies unveil PTHrP-vitamin D-RAS axis as pivotal in the CAF
    Article Snippet: Sequencing was performed using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles; cat. no. 20028318; Illumina, Inc.). .. Final library concentrations were quantified using the KAPA Library Quantification Kit (cat. no. KK4824/D; Roche Diagnostics) by real-time PCR. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Exploring CSF microRNA signatures as diagnostic biomarkers in adult-type diffuse gliomas
    Article Snippet: .. Pooled libraries were quantified by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems). .. The Illumina NextSeq 500 was used to sequence the pooled library, one pool per run, in a single-end configuration, with a read length of 75 bp.

    Article Title: Whole-exome sequencing unveils novel potential gene mutations involved in primary renal small cell carcinoma
    Article Snippet: .. The concentration was accurately measured using the Qubit ® dsDNA HS Assay Kit (Thermo Fisher Scientific) and confirmed by quantitative PCR (qPCR) using the KAPA Library Quantification Kit (Roche, Catalog No. KK4824) to ensure precise molarity calculation for cluster generation. ..

    Article Title: Hypoxic CAF studies unveil PTHrP-vitamin D-RAS axis as pivotal in the CAF
    Article Snippet: Sequencing was performed using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles; cat. no. 20028318; Illumina, Inc.). .. Final library concentrations were quantified using the KAPA Library Quantification Kit (cat. no. KK4824/D; Roche Diagnostics) by real-time PCR. ..

    Concentration Assay:

    Article Title: Whole-exome sequencing unveils novel potential gene mutations involved in primary renal small cell carcinoma
    Article Snippet: .. The concentration was accurately measured using the Qubit ® dsDNA HS Assay Kit (Thermo Fisher Scientific) and confirmed by quantitative PCR (qPCR) using the KAPA Library Quantification Kit (Roche, Catalog No. KK4824) to ensure precise molarity calculation for cluster generation. ..

    Article Title: Cell culture methods and compositions
    Article Snippet: To remove excess primers and adaptor dimers, PCR products were purified twice using SPRI beads (Beckman Coulter). .. The quality of the libraries was assessed using Agilent Bioanalyzer DNA 1000 chip (Duke-NUS Genome Facility) and the molar concentration of each library was determined using KAPA library quantification kit (Roche). .. Three pooled libraries (each containing 9 samples with the pooled concentration of 8 nM in 30 uL) was sent for sequencing on Illumina HiSeq4000 (NovogeneAIT Genomics Singapore).

    Sequencing:

    Article Title: Small regulatory RNAs modulate lactococcal susceptibility to cell wall-targeting antimicrobials
    Article Snippet: After ethanol precipitation, RNA was treated with RNA 5′ pyrophosphohydrolase (RppH) (New England Biolabs), recovered with RNA Clean & Concentrator-5 Kit, and 165 ng were taken for further processing using NEXTflex Small RNA-Seq Kit v3 (PerkinElmer) with the following modifications: at Step E, incubation at 42°C was extended to 60 min; at Step G, 16 polymerase chain reaction (PCR) cycles were performed; and Steps F and H1 were carried out as described in the alternative protocol for Preparing Libraries without Size Selection. .. The resulting complementary DNA (cDNA) libraries were quantified by KAPATM Library Quantification Kit (Roche) and sequenced on the Illumina MiSeqTM instrument using MiSeqTM Reagent Kit v2 chemistry (Illumina) for paired-end sequencing with 2 × 151 bp reads. ..



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