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Pacific Biosciences pacbio full length transcript sequencing
Pacbio Full Length Transcript Sequencing, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/length+transcript+sequences/full+kinnex+kit+length+rna/pmc12614215-60-32-30
Average 86 stars, based on 1 article reviews
pacbio full length transcript sequencing - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Sequencing:

Article Title: EMITS: expectation-maximization abundance estimation for fungal ITS communities from long-read sequencing
Article Snippet: .. Advances in long-read sequencing, particularly Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), have made full-length ITS amplicon sequencing increasingly accessible, offering improved taxonomic resolution over short-read approaches that target only the ITS1 or ITS2 subregion [ , ]. ..

Article Title: Tonotopic specialization of MYO7A isoforms in auditory hair cells.
Article Snippet: .. The Kinnex kit (103-072-200, Pacific Biosciences, CA, USA) was used to generate concatemers for long-read sequencing. .. Library quality was assessed using a Qubit fluorometer (Q33238, Thermo Fisher Scientific, MA, USA) and an Agilent 2100 Bioanalyzer (G2939BA, Agilent Technologies, CA, USA).

Article Title: Effects of 16S rRNA hypervariable region selection on respiratory microbiome profiling in healthy adults.
Article Snippet: Selection of the 16S rRNA gene target region can substantially influence inferred respiratory microbiome profiles, particularly in low-biomass samples.. In this study, we compared full-length V1-V9 (Pacific Biosciences) and V3-V4 (Illumina) amplicon sequencing using exhaled breath condensate collected from 50 healthy adults.. Samples that yielded successful amplification for both approaches were included in paired, within-subject comparisons of sequencing depth, alpha diversity, and taxonomic composition.

Article Title: Human antibody targeting Crimean-Congo hemorrhagic fever virus glycoprotein 38 protects mice against heterologous virus challenge.
Article Snippet: Using the AMPure Size Select Magnetic Bead Kit (ratio of 0.6× (Beckman Coulter)), the first PCR reaction was purified. .. Amplicon libraries were prepared per the Multiplex SMRT Sequencing protocol (Pacific Biosciences) and sequenced on a Sequel instrument (Pacific Biosciences). .. Raw sequences were demultiplexed, and circular consensus sequences were determined using SMRT Analysis tool suite (Pacific Biosciences).

Article Title: Human antibody targeting Crimean-Congo hemorrhagic fever virus glycoprotein 38 protects mice against heterologous virus challenge
Article Snippet: Using the AMPure Size Select Magnetic Bead Kit (ratio of 0.6× (Beckman Coulter)), the first PCR reaction was purified. .. Amplicon libraries were prepared per the Multiplex SMRT Sequencing protocol (Pacific Biosciences) and sequenced on a Sequel instrument (Pacific Biosciences). .. Raw sequences were demultiplexed, and circular consensus sequences were determined using SMRT Analysis tool suite (Pacific Biosciences).

Article Title: Acarbose redirects gut microbiome utilization of dietary carbohydrates to suppress anaphylaxis in mice.
Article Snippet: .. Full-length 16S rRNA gene amplicon sequencing Full-length 16S rRNA genes were amplified using the Pacific Biosciences (PacBio) barcoded forward primers (5′-Phos-GCATCNNNN NNNNNNAGRGTTYGATYMTGGCTCAG-3′) and 1492Rmod (5′-PhosGCATCNNNNNNNNNNRGHTACCTTGTTACGACTT-3′). ..

Amplification:

Article Title: EMITS: expectation-maximization abundance estimation for fungal ITS communities from long-read sequencing
Article Snippet: .. Advances in long-read sequencing, particularly Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), have made full-length ITS amplicon sequencing increasingly accessible, offering improved taxonomic resolution over short-read approaches that target only the ITS1 or ITS2 subregion [ , ]. ..

Article Title: Effects of 16S rRNA hypervariable region selection on respiratory microbiome profiling in healthy adults.
Article Snippet: Selection of the 16S rRNA gene target region can substantially influence inferred respiratory microbiome profiles, particularly in low-biomass samples.. In this study, we compared full-length V1-V9 (Pacific Biosciences) and V3-V4 (Illumina) amplicon sequencing using exhaled breath condensate collected from 50 healthy adults.. Samples that yielded successful amplification for both approaches were included in paired, within-subject comparisons of sequencing depth, alpha diversity, and taxonomic composition.

Article Title: Human antibody targeting Crimean-Congo hemorrhagic fever virus glycoprotein 38 protects mice against heterologous virus challenge.
Article Snippet: Using the AMPure Size Select Magnetic Bead Kit (ratio of 0.6× (Beckman Coulter)), the first PCR reaction was purified. .. Amplicon libraries were prepared per the Multiplex SMRT Sequencing protocol (Pacific Biosciences) and sequenced on a Sequel instrument (Pacific Biosciences). .. Raw sequences were demultiplexed, and circular consensus sequences were determined using SMRT Analysis tool suite (Pacific Biosciences).

Article Title: Human antibody targeting Crimean-Congo hemorrhagic fever virus glycoprotein 38 protects mice against heterologous virus challenge
Article Snippet: Using the AMPure Size Select Magnetic Bead Kit (ratio of 0.6× (Beckman Coulter)), the first PCR reaction was purified. .. Amplicon libraries were prepared per the Multiplex SMRT Sequencing protocol (Pacific Biosciences) and sequenced on a Sequel instrument (Pacific Biosciences). .. Raw sequences were demultiplexed, and circular consensus sequences were determined using SMRT Analysis tool suite (Pacific Biosciences).

Article Title: Acarbose redirects gut microbiome utilization of dietary carbohydrates to suppress anaphylaxis in mice.
Article Snippet: .. Full-length 16S rRNA gene amplicon sequencing Full-length 16S rRNA genes were amplified using the Pacific Biosciences (PacBio) barcoded forward primers (5′-Phos-GCATCNNNN NNNNNNAGRGTTYGATYMTGGCTCAG-3′) and 1492Rmod (5′-PhosGCATCNNNNNNNNNNRGHTACCTTGTTACGACTT-3′). ..

Multiplex Assay:

Article Title: Human antibody targeting Crimean-Congo hemorrhagic fever virus glycoprotein 38 protects mice against heterologous virus challenge.
Article Snippet: Using the AMPure Size Select Magnetic Bead Kit (ratio of 0.6× (Beckman Coulter)), the first PCR reaction was purified. .. Amplicon libraries were prepared per the Multiplex SMRT Sequencing protocol (Pacific Biosciences) and sequenced on a Sequel instrument (Pacific Biosciences). .. Raw sequences were demultiplexed, and circular consensus sequences were determined using SMRT Analysis tool suite (Pacific Biosciences).

Article Title: Human antibody targeting Crimean-Congo hemorrhagic fever virus glycoprotein 38 protects mice against heterologous virus challenge
Article Snippet: Using the AMPure Size Select Magnetic Bead Kit (ratio of 0.6× (Beckman Coulter)), the first PCR reaction was purified. .. Amplicon libraries were prepared per the Multiplex SMRT Sequencing protocol (Pacific Biosciences) and sequenced on a Sequel instrument (Pacific Biosciences). .. Raw sequences were demultiplexed, and circular consensus sequences were determined using SMRT Analysis tool suite (Pacific Biosciences).



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