full-length transcript isoform-sequencing (iso-seq) (Pacific Biosciences)
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Pacific Biosciences
full-length transcript isoform-sequencing (iso-seq)
Full Length Transcript Isoform Sequencing (Iso Seq), supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/length+transcript+sequences/isoform+sequencing/pmc11960707-49-8-6
Average 90 stars, based on 1 article reviews
Full Length Transcript Isoform Sequencing (Iso Seq), supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/length+transcript+sequences/isoform+sequencing/pmc11960707-49-8-6
Average 90 stars, based on 1 article reviews
full-length transcript isoform-sequencing (iso-seq) - by Bioz Stars,
2026-11
90/100 stars
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Sequencing:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. RNA Sequencing:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Activation Assay:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Variant Assay:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. RNA Sequencing Assay:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Expressing:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Standard Deviation:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Ex Vivo:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Inhibition:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Generated:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Transfection:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Staining:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Plasmid Preparation:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. Mutagenesis:Article Title: Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducing Spirodela polyrhiza Article Snippet: We further performed long-read cDNA-seq with Article Title: Full-length mRNA sequencing resolves novel variation in 5′ UTR length for genes expressed during human CD4 T - cell activation Article Snippet: Article Title: Investigating RNA dynamics from single molecule transcriptomes. Article Snippet: Advances in experimental protocols for studying diverse RNA modalities and their associated data streams have driven the development of a new generation of computational tools.. Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation.. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. Article Title: Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod ( Oxyeleotris lineolata ). Article Snippet: Thus, the Article Title: Comprehensive analysis of Metacrinus rotundus full length transcriptome. Article Snippet: To reveal the complexity of the FL reference transcriptome of M. rotundus more comprehensively, we applied long-read Article Title: A tumour necrosis factor-α responsive cryptic promoter drives overexpression of the human endogenous retrovirus ERVK-7. Article Snippet: To do so, we obtained Article Title: Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolution. Article Snippet: longand short-read isoform sequencing at bulk or single-cell resolution Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, Aziz M. Al’Khafaji, and Brian J. Haas Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA; Department of Biosystems Science and Engineering, ETH Zurich, 4056 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 4056 Basel, Switzerland Article Title: Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: insights into slow-twitch and fast-twitch muscle specialization. Article Snippet: Alternative splicing (AS) plays a crucial role in regulating muscle type specialization characteristics in mammals but has been rarely explored in teleost.. In this study, we combined Iso-Seq and RNA-Seq technologies to profile the transcriptome and AS landscape of slow-twitch muscle (SM) and fast-twitch muscle (FM) in a migratory teleost, Pseudocaranx dentex.. We identified 24,096 full-length transcripts and 14,346 isoforms in SM, and 18,483 full-length transcripts and 10,541 isoforms in FM, revealing extensive transcript and isoform diversity. |