Review



lav blot 1 test kit  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Bio-Rad lav blot 1 test kit
    Lav Blot 1 Test Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/System+Test+Kit/pmc06789618-57-7-12
    Average 94 stars, based on 158 article reviews
    lav blot 1 test kit - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Quantitative bead–based multiplex assay for simultaneous determination of IgG concentrations of pertussis toxin, filamentous hemagglutinin, pertactin, diphtheria, tetanus, Haemophilus influenzae b, and hepatitis B in human serum samples
    Article Snippet: .. The Bio-Rad ELISA test kit was used for the quantification of human antibodies against the Hep B IgG class in 09/222, 07/164, and MRS. ..

    Article Title: Isolation of recombinant HIV-1 Rev protein and investigation of a new class of benzimidazole inhibitors capability to disrupt Rev-RRE complex.
    Article Snippet: In the present study, an efficient method for the expression and purification of recombinant HIV Rev protein with a C-terminal hexahistidine tag was proposed.. Noteworthy, this method circumvents the precipitation of the protein into inclusion bodies and their subsequent aggregation during purification.. It does not necessitate denaturing isolation conditions, in contrast to currently widely used protocols.

    Software:

    Article Title:
    Article Snippet: Geneoscopy, Inc. 2220 Welsch Industrial Ct. St. Louis, MO 63146 www.geneoscopy.com 314-887-7777 ColoSense Clinician Brochure® For In-Vitro Diagnostic Use Rx Only Prescription Use Only ColoSense Clinician Brochure® Table of Contents Intended Use / Indication for Use Contraindications Warnings and Precautions ColoSense Overview Patient Samples for ColoSense ColoSense Patient Services Colorectal Cancer Overview Device Description Clinical Study: The ColoRectal Cancer and PRE-Cancerous Adenoma Non-InvasiVE DetectioN Test (CRC-PREVENT) Overview Study Design Study Population Clinical Validation of ColoSense Clinical Performance Measures and Related Endpoints Study Population and Baseline Demographics (Clinical Effectiveness Population) Performance Characteristics for the Average-risk Cohort (Clinical Effectiveness Population) Performance in the Non-Average-risk Cohort Cross-Reactivity Interfering Substances Ordering ColoSense Instructions for Sample Collection Interpretation of ColoSense Results Positive Result Negative Result No Result References 1 1 2 3 4 4 4 5 6 6 6 6 7 7 7 9 11 13 14 14 15 16 16 16 16 17 Intended Use / Indications For Use ColoSense is intended for the qualitative detection of colorectal neoplasia-associated RNA markers and for the presence of occult hemoglobin in human stool. .. ColoSense is for use with the ColoSense Collection Kit, the ColoSense Test Kit, the ColoSense Software, and the following instruments: Polymedco iFOBT Analyzer; bioMérieux EMAG Nucleic Acid Extraction System; and Bio-Rad QXDx ddPCR System. .. ColoSense is a singlesite test performed at Geneoscopy, Inc. A positive ColoSense result may indicate the presence of colorectal cancer (CRC), advanced adenomas (AA), or serrated precancerous lesions (SPL) and should be followed by a colonoscopy.

    Extraction:

    Article Title:
    Article Snippet: Geneoscopy, Inc. 2220 Welsch Industrial Ct. St. Louis, MO 63146 www.geneoscopy.com 314-887-7777 ColoSense Clinician Brochure® For In-Vitro Diagnostic Use Rx Only Prescription Use Only ColoSense Clinician Brochure® Table of Contents Intended Use / Indication for Use Contraindications Warnings and Precautions ColoSense Overview Patient Samples for ColoSense ColoSense Patient Services Colorectal Cancer Overview Device Description Clinical Study: The ColoRectal Cancer and PRE-Cancerous Adenoma Non-InvasiVE DetectioN Test (CRC-PREVENT) Overview Study Design Study Population Clinical Validation of ColoSense Clinical Performance Measures and Related Endpoints Study Population and Baseline Demographics (Clinical Effectiveness Population) Performance Characteristics for the Average-risk Cohort (Clinical Effectiveness Population) Performance in the Non-Average-risk Cohort Cross-Reactivity Interfering Substances Ordering ColoSense Instructions for Sample Collection Interpretation of ColoSense Results Positive Result Negative Result No Result References 1 1 2 3 4 4 4 5 6 6 6 6 7 7 7 9 11 13 14 14 15 16 16 16 16 17 Intended Use / Indications For Use ColoSense is intended for the qualitative detection of colorectal neoplasia-associated RNA markers and for the presence of occult hemoglobin in human stool. .. ColoSense is for use with the ColoSense Collection Kit, the ColoSense Test Kit, the ColoSense Software, and the following instruments: Polymedco iFOBT Analyzer; bioMérieux EMAG Nucleic Acid Extraction System; and Bio-Rad QXDx ddPCR System. .. ColoSense is a singlesite test performed at Geneoscopy, Inc. A positive ColoSense result may indicate the presence of colorectal cancer (CRC), advanced adenomas (AA), or serrated precancerous lesions (SPL) and should be followed by a colonoscopy.

    Infection:

    Article Title: Isolation of recombinant HIV-1 Rev protein and investigation of a new class of benzimidazole inhibitors capability to disrupt Rev-RRE complex.
    Article Snippet: In the present study, an efficient method for the expression and purification of recombinant HIV Rev protein with a C-terminal hexahistidine tag was proposed.. Noteworthy, this method circumvents the precipitation of the protein into inclusion bodies and their subsequent aggregation during purification.. It does not necessitate denaturing isolation conditions, in contrast to currently widely used protocols.

    Article Title: Anti-HIV and Antimicrobial Activity of 7-Hydrazino-8-hydroxyquinoline-Based Aromatic Hydrazones
    Article Snippet: .. The final ethanol concentration was 1% v / v . On the third day after infection, the culture fluid was collected for determining the amount of p24 antigen by enzyme immunoassay analysis using a commercial test system kit (Genscreen ULTRA HIV Ag-Ab, Bio-Rad, Hercules, CA, USA). ..

    other:

    Article Title: Anti-HIV and Antimicrobial Activity of 7-Hydrazino-8-hydroxyquinoline-Based Aromatic Hydrazones.
    Article Snippet: Candida albicans ATCC 10231, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213 strains were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

    Diagnostic Assay:

    Article Title: Real-Time Quaking-Induced Conversion Assay Applied to the Italian Chronic Wasting Disease Monitoring Plan: Comparison of Classical and Innovative Diagnostic Methods
    Article Snippet: .. Based on the protocol established in this study, all samples that tested positive or doubtful in the applied diagnostic tests were retested with both the Idexx test, RT-QuIC, and, in addition, the rapid test TeSeETM SAP Combi Kit (Bio-Rad, Hercules, CA, USA) and two confirmatory tests, Western Blot (WB) and Immunohistochemistry (IHC). ..

    Western Blot:

    Article Title: Real-Time Quaking-Induced Conversion Assay Applied to the Italian Chronic Wasting Disease Monitoring Plan: Comparison of Classical and Innovative Diagnostic Methods
    Article Snippet: .. Based on the protocol established in this study, all samples that tested positive or doubtful in the applied diagnostic tests were retested with both the Idexx test, RT-QuIC, and, in addition, the rapid test TeSeETM SAP Combi Kit (Bio-Rad, Hercules, CA, USA) and two confirmatory tests, Western Blot (WB) and Immunohistochemistry (IHC). ..

    Immunohistochemistry:

    Article Title: Real-Time Quaking-Induced Conversion Assay Applied to the Italian Chronic Wasting Disease Monitoring Plan: Comparison of Classical and Innovative Diagnostic Methods
    Article Snippet: .. Based on the protocol established in this study, all samples that tested positive or doubtful in the applied diagnostic tests were retested with both the Idexx test, RT-QuIC, and, in addition, the rapid test TeSeETM SAP Combi Kit (Bio-Rad, Hercules, CA, USA) and two confirmatory tests, Western Blot (WB) and Immunohistochemistry (IHC). ..

    Concentration Assay:

    Article Title: Anti-HIV and Antimicrobial Activity of 7-Hydrazino-8-hydroxyquinoline-Based Aromatic Hydrazones
    Article Snippet: .. The final ethanol concentration was 1% v / v . On the third day after infection, the culture fluid was collected for determining the amount of p24 antigen by enzyme immunoassay analysis using a commercial test system kit (Genscreen ULTRA HIV Ag-Ab, Bio-Rad, Hercules, CA, USA). ..

    Enzyme Immunoassay:

    Article Title: Anti-HIV and Antimicrobial Activity of 7-Hydrazino-8-hydroxyquinoline-Based Aromatic Hydrazones
    Article Snippet: .. The final ethanol concentration was 1% v / v . On the third day after infection, the culture fluid was collected for determining the amount of p24 antigen by enzyme immunoassay analysis using a commercial test system kit (Genscreen ULTRA HIV Ag-Ab, Bio-Rad, Hercules, CA, USA). ..

    Analysis:

    Article Title: Anti-HIV and Antimicrobial Activity of 7-Hydrazino-8-hydroxyquinoline-Based Aromatic Hydrazones
    Article Snippet: .. The final ethanol concentration was 1% v / v . On the third day after infection, the culture fluid was collected for determining the amount of p24 antigen by enzyme immunoassay analysis using a commercial test system kit (Genscreen ULTRA HIV Ag-Ab, Bio-Rad, Hercules, CA, USA). ..



    Similar Products

    97
    Bio-Rad lav blot i kit
    Lav Blot I Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/Bio-Rad+Protein+Assay+Kit+I/10__1016_slash_s2352___3018_ascii40_24_ascii41_00090___0-47-29-33
    Average 97 stars, based on 1 article reviews
    lav blot i kit - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    90
    Bio-Rad lav blot 1 kit
    Lav Blot 1 Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/Western+Blot+Temperature+Sensitive+Reagents+Bag/pmc02726149-101-32-36
    Average 90 stars, based on 1 article reviews
    lav blot 1 kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Bio-Rad lav blot hiv-1 western blotting kit
    The <t>HIV-1</t> subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.
    Lav Blot Hiv 1 Western Blotting Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/Western+Blot+Temperature+Sensitive+Reagents+Bag/pmc02708135-97-9-16
    Average 90 stars, based on 1 article reviews
    lav blot hiv-1 western blotting kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Bio-Rad lav blot kits
    The <t>HIV-1</t> subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.
    Lav Blot Kits, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/Western+Blot+Temperature+Sensitive+Reagents+Bag/pmc03167974-48-10-9
    Average 90 stars, based on 1 article reviews
    lav blot kits - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    94
    Bio-Rad lav blot 1 test kit
    The <t>HIV-1</t> subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.
    Lav Blot 1 Test Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/System+Test+Kit/pmc06789618-57-7-12
    Average 94 stars, based on 1 article reviews
    lav blot 1 test kit - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    90
    Bio-Rad lav blot kit
    The <t>HIV-1</t> subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.
    Lav Blot Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/Comparative+Proteomics+Kit+II%3A+Western+Blot+Module/pmc06300218-161-13-17
    Average 90 stars, based on 1 article reviews
    lav blot kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Bio-Rad hiv strips (lav blot kit
    The <t>HIV-1</t> subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.
    Hiv Strips (Lav Blot Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/hiv+strips++lav+blot+kit/us09555097-548-16-23
    Average 90 stars, based on 1 article reviews
    hiv strips (lav blot kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Bio-Rad lav blot 2 kit
    The <t>HIV-1</t> subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.
    Lav Blot 2 Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lav+blot+kit/Western+Blot+Temperature+Sensitive+Reagents+Bag/pmc04313141-38-38-42
    Average 90 stars, based on 1 article reviews
    lav blot 2 kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    The HIV-1 subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.

    Journal: Virology Journal

    Article Title: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

    doi: 10.1186/1743-422X-6-87

    Figure Lengend Snippet: The HIV-1 subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella . (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac ( lactose ) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys ® HIV p24 Ag assay. In the Roche Elecsys ® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys ® 2010 analyzer using readings from the negative and positive calibrators.

    Article Snippet: HIV-1 Gag-specific IgG responses were confirmed using the New LAV Blot I HIV-1 Western blotting kit (Biorad).

    Techniques: Expressing, Plasmid Preparation, Recombinant, SDS Page

    HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by IFN-γ and IL-4 ELISPOT assays . Groups of mice (5 mice per group) were vaccinated three times (day 0, 28 and 56) with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM). Mice were sacrificed on day 84, and splenocytes were prepared from isolated spleens pooled from 5 mice per group then used in IFN-γ (A) and IL-4 (B) ELISPOT assays with R10 medium only (negative assay control) or with the Gag CD4 peptide. Bars are the mean number of spots from triplicate wells and are expressed as SFU/10e6 splenocytes. Differences in immune responses between vaccine groups were analyzed by a two-sample student's t-test. A p < 0.05 was considered statistically significant. The differences in response between stimulated cells and unstimulated cells within the same vaccine group were also analyzed by a student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

    Journal: Virology Journal

    Article Title: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

    doi: 10.1186/1743-422X-6-87

    Figure Lengend Snippet: HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by IFN-γ and IL-4 ELISPOT assays . Groups of mice (5 mice per group) were vaccinated three times (day 0, 28 and 56) with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM). Mice were sacrificed on day 84, and splenocytes were prepared from isolated spleens pooled from 5 mice per group then used in IFN-γ (A) and IL-4 (B) ELISPOT assays with R10 medium only (negative assay control) or with the Gag CD4 peptide. Bars are the mean number of spots from triplicate wells and are expressed as SFU/10e6 splenocytes. Differences in immune responses between vaccine groups were analyzed by a two-sample student's t-test. A p < 0.05 was considered statistically significant. The differences in response between stimulated cells and unstimulated cells within the same vaccine group were also analyzed by a student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

    Article Snippet: HIV-1 Gag-specific IgG responses were confirmed using the New LAV Blot I HIV-1 Western blotting kit (Biorad).

    Techniques: Enzyme-linked Immunospot, Recombinant, Isolation

    HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by the amount of cytokines secreted by stimulated cells . Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Splenocytes isolated and pooled from 5 mice per group on day 84 were incubated in R10 medium only (negative assay control), or stimulated with a Gag CD4 peptide for 48 hrs. Cytokines released into the supernatant were quantified using a mouse Th1/Th2 cytokine bead array (CBA) assay. (A) IFN-γ, (B) TNF-α, (C) IL-4 and (D) IL-5. Each bar in the graphs represents the average picogram amount of cytokine produced per 10e6 splenocytes in 48 hours of stimulation with media or Gag CD4 peptide, for triplicate responses. A p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

    Journal: Virology Journal

    Article Title: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

    doi: 10.1186/1743-422X-6-87

    Figure Lengend Snippet: HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by the amount of cytokines secreted by stimulated cells . Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Splenocytes isolated and pooled from 5 mice per group on day 84 were incubated in R10 medium only (negative assay control), or stimulated with a Gag CD4 peptide for 48 hrs. Cytokines released into the supernatant were quantified using a mouse Th1/Th2 cytokine bead array (CBA) assay. (A) IFN-γ, (B) TNF-α, (C) IL-4 and (D) IL-5. Each bar in the graphs represents the average picogram amount of cytokine produced per 10e6 splenocytes in 48 hours of stimulation with media or Gag CD4 peptide, for triplicate responses. A p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

    Article Snippet: HIV-1 Gag-specific IgG responses were confirmed using the New LAV Blot I HIV-1 Western blotting kit (Biorad).

    Techniques: Recombinant, Isolation, Incubation, Produced

    HIV-1 subtype C Gag-specific serum IgG responses in mice vaccinated with recombinant Salmonella vaccine vector . Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Serum (pooled from 5 mice per group) was isolated from blood taken before each vaccination on day 0, 28 and 56 and just before sacrifice on day 84. (A) The HIV-1 Gag-specific IgG for each group of mice with a 1/100 serum dilution. The data are the ratio of the OD 405 nm for vaccinated mice and the OD 405 nm for the day 0 serum (pre-bleed). (B) The HIV-1 Gag-specific IgG1 and IgG2a were measured in serum of each group of mice on Day 84 with a 1/100 serum dilution. Each bar represents the mean OD 405 nm value. Differences in antibody responses between vaccine groups at different time points were analyzed by a two-sample student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

    Journal: Virology Journal

    Article Title: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

    doi: 10.1186/1743-422X-6-87

    Figure Lengend Snippet: HIV-1 subtype C Gag-specific serum IgG responses in mice vaccinated with recombinant Salmonella vaccine vector . Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Serum (pooled from 5 mice per group) was isolated from blood taken before each vaccination on day 0, 28 and 56 and just before sacrifice on day 84. (A) The HIV-1 Gag-specific IgG for each group of mice with a 1/100 serum dilution. The data are the ratio of the OD 405 nm for vaccinated mice and the OD 405 nm for the day 0 serum (pre-bleed). (B) The HIV-1 Gag-specific IgG1 and IgG2a were measured in serum of each group of mice on Day 84 with a 1/100 serum dilution. Each bar represents the mean OD 405 nm value. Differences in antibody responses between vaccine groups at different time points were analyzed by a two-sample student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

    Article Snippet: HIV-1 Gag-specific IgG responses were confirmed using the New LAV Blot I HIV-1 Western blotting kit (Biorad).

    Techniques: Recombinant, Plasmid Preparation, Isolation