rneasy plant mini kit  (Qiagen)

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: Expressing, Plasmid Preparation, Recombinant, Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Purification, Positive Control, Negative Control

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: Immunostaining, Plasmid Preparation

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: Transfection, Plasmid Preparation

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: Transfection, Plasmid Preparation, Construct, Staining, Immunostaining, Fluorescence, Microscopy

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: ( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques:

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: ( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: TUNEL Assay, Activity Assay, Infection, Plasmid Preparation