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lambda zap express vector  (Agilent technologies)


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    Agilent technologies lambda zap express vector
    Lambda Zap Express Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda-zap+express+vector/pmc02920863-45-6-10
    Average 90 stars, based on 1 article reviews
    lambda zap express vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: Differential expression of lysosome-associated protein transmembrane-4 beta (LAPTM4B) in granulosa cells of ovarian follicles and in other bovine tissues
    Article Snippet: .. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as previously described [ ]. .. Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were randomly picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON).

    Article Title: Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea
    Article Snippet: .. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as described [ ]. .. Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON).

    Article Title: Functional analysis of a novel glioma antigen, EFTUD1
    Article Snippet: Short tandem repeat DNA profiling of T98G and U87MG cells, a kind gift from collaborators (Keio University), was performed using the Cell ID System (Promega). .. Immunoscreening of the cDNA Library With SEREX and Identification of Glioma Antigen Total RNA was extracted from cultured glioma cell lines (U87MG and T98G) by the guanidinium thiocyanate method, and poly(A) + RNA was purified. cDNA was ligated into a lambda ZAP expression vector (Stratagene), and a lambda-phage cDNA library was obtained through in vitro packaging. ..

    Article Title: Human PDE4A8, a novel brain-expressed PDE4 cAMP-specific phosphodiesterase that has undergone rapid evolutionary change
    Article Snippet: .. A human fetal brain cDNA library (Stratagene), cloned into the EcoRI site of the Lambda ZAP vector (Stratagene), was screened by hybridization using a probe corresponding to a portion of the human PDE4C catalytic region and 3′ sequences (the 1155 nt full-length insert of pPDE21 [ 10 ]; GenBank ® accession number L20968 ). ..

    Construct:

    Article Title: Differential expression of lysosome-associated protein transmembrane-4 beta (LAPTM4B) in granulosa cells of ovarian follicles and in other bovine tissues
    Article Snippet: .. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as previously described [ ]. .. Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were randomly picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON).

    Article Title: Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea
    Article Snippet: .. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as described [ ]. .. Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON).

    Plasmid Preparation:

    Article Title: Differential expression of lysosome-associated protein transmembrane-4 beta (LAPTM4B) in granulosa cells of ovarian follicles and in other bovine tissues
    Article Snippet: .. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as previously described [ ]. .. Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were randomly picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON).

    Article Title: Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea
    Article Snippet: .. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as described [ ]. .. Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON).

    Article Title: Functional analysis of a novel glioma antigen, EFTUD1
    Article Snippet: Short tandem repeat DNA profiling of T98G and U87MG cells, a kind gift from collaborators (Keio University), was performed using the Cell ID System (Promega). .. Immunoscreening of the cDNA Library With SEREX and Identification of Glioma Antigen Total RNA was extracted from cultured glioma cell lines (U87MG and T98G) by the guanidinium thiocyanate method, and poly(A) + RNA was purified. cDNA was ligated into a lambda ZAP expression vector (Stratagene), and a lambda-phage cDNA library was obtained through in vitro packaging. ..

    Article Title: Human PDE4A8, a novel brain-expressed PDE4 cAMP-specific phosphodiesterase that has undergone rapid evolutionary change
    Article Snippet: .. A human fetal brain cDNA library (Stratagene), cloned into the EcoRI site of the Lambda ZAP vector (Stratagene), was screened by hybridization using a probe corresponding to a portion of the human PDE4C catalytic region and 3′ sequences (the 1155 nt full-length insert of pPDE21 [ 10 ]; GenBank ® accession number L20968 ). ..

    Article Title: Gene discovery within the planctomycete division of the domain Bacteria using sequence tags from genomic DNA libraries
    Article Snippet: .. The restricted DNA fragments were ligated into the Bam HI arms of the lambda Zap Express vector (Stratagene). .. The vector DNA, containing inserts, was packaged into lambda phage heads using Gigapack III Gold packaging extract (Stratagene).

    Cloning:

    Article Title: Differential expression of lysosome-associated protein transmembrane-4 beta (LAPTM4B) in granulosa cells of ovarian follicles and in other bovine tissues
    Article Snippet: .. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as previously described [ ]. .. Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were randomly picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON).

    Article Title: Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea
    Article Snippet: .. The cDNA library was constructed in lambda Zap Express vector (Stratagene, La Jolla, CA) by unidirectional cloning of cDNAs as described [ ]. .. Following in vivo excision of pBluescript phagemids containing the cloned cDNA insert with the Ex-Assist/XLOLR system (Stratagene), single bacterial colonies were picked and their phagemid content were purified by mini-prep (Qiagen, Mississauga, ON).

    other:

    Article Title: An EST screen from the annelid Pomatoceros lamarckii reveals patterns of gene loss and gain in animals
    Article Snippet: The cDNA library was then cloned into the XhoI/EcoRI site of the Lambda-Zap Express vector (Stratagene, California, USA).

    Article Title: A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution
    Article Snippet: Messenger RNA from approximately 10 8 cells of S. salmonicida was isolated using the Dynabead mRNA system (Dynal), and cDNA was synthesized and cloned into the lambda Uni-Zap XR vector (Stratagene), according to the manufacturer's instructions.

    Article Title: The gene diversity pattern ofDiplocarpon rosaepopulations is shaped by the age, diversity and fungicide treatment of their host populations
    Article Snippet: Black spot disease caused by the hemibiotrophic ascomycete Diplocarpon rosae is the most devastating disease of fieldgrown roses.. Although resistance to black spot is an important trait for rose breeding, little information on the diversity of the pathogen is currently available.. To date, a number of single-spore isolates have been characterized based on a set of test genotypes of the host.

    Cell Culture:

    Article Title: Functional analysis of a novel glioma antigen, EFTUD1
    Article Snippet: Short tandem repeat DNA profiling of T98G and U87MG cells, a kind gift from collaborators (Keio University), was performed using the Cell ID System (Promega). .. Immunoscreening of the cDNA Library With SEREX and Identification of Glioma Antigen Total RNA was extracted from cultured glioma cell lines (U87MG and T98G) by the guanidinium thiocyanate method, and poly(A) + RNA was purified. cDNA was ligated into a lambda ZAP expression vector (Stratagene), and a lambda-phage cDNA library was obtained through in vitro packaging. ..

    Purification:

    Article Title: Functional analysis of a novel glioma antigen, EFTUD1
    Article Snippet: Short tandem repeat DNA profiling of T98G and U87MG cells, a kind gift from collaborators (Keio University), was performed using the Cell ID System (Promega). .. Immunoscreening of the cDNA Library With SEREX and Identification of Glioma Antigen Total RNA was extracted from cultured glioma cell lines (U87MG and T98G) by the guanidinium thiocyanate method, and poly(A) + RNA was purified. cDNA was ligated into a lambda ZAP expression vector (Stratagene), and a lambda-phage cDNA library was obtained through in vitro packaging. ..

    Expressing:

    Article Title: Functional analysis of a novel glioma antigen, EFTUD1
    Article Snippet: Short tandem repeat DNA profiling of T98G and U87MG cells, a kind gift from collaborators (Keio University), was performed using the Cell ID System (Promega). .. Immunoscreening of the cDNA Library With SEREX and Identification of Glioma Antigen Total RNA was extracted from cultured glioma cell lines (U87MG and T98G) by the guanidinium thiocyanate method, and poly(A) + RNA was purified. cDNA was ligated into a lambda ZAP expression vector (Stratagene), and a lambda-phage cDNA library was obtained through in vitro packaging. ..

    In Vitro:

    Article Title: Functional analysis of a novel glioma antigen, EFTUD1
    Article Snippet: Short tandem repeat DNA profiling of T98G and U87MG cells, a kind gift from collaborators (Keio University), was performed using the Cell ID System (Promega). .. Immunoscreening of the cDNA Library With SEREX and Identification of Glioma Antigen Total RNA was extracted from cultured glioma cell lines (U87MG and T98G) by the guanidinium thiocyanate method, and poly(A) + RNA was purified. cDNA was ligated into a lambda ZAP expression vector (Stratagene), and a lambda-phage cDNA library was obtained through in vitro packaging. ..

    Hybridization:

    Article Title: Human PDE4A8, a novel brain-expressed PDE4 cAMP-specific phosphodiesterase that has undergone rapid evolutionary change
    Article Snippet: .. A human fetal brain cDNA library (Stratagene), cloned into the EcoRI site of the Lambda ZAP vector (Stratagene), was screened by hybridization using a probe corresponding to a portion of the human PDE4C catalytic region and 3′ sequences (the 1155 nt full-length insert of pPDE21 [ 10 ]; GenBank ® accession number L20968 ). ..



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    ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and <t>apoptin</t> transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ <t>ZAP-CMV</t> vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.
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    ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Expressing, Plasmid Preparation, Recombinant, Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Purification, Positive Control, Negative Control

    Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Immunostaining, Plasmid Preparation

    BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Transfection, Plasmid Preparation

    BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Transfection, Plasmid Preparation, Construct, Staining, Immunostaining, Fluorescence, Microscopy

    ( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques:

    ( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: TUNEL Assay, Activity Assay, Infection, Plasmid Preparation