lambda zap ii vector at xhoi neb (New England Biolabs)
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New England Biolabs
lambda zap ii vector at xhoi neb
Lambda Zap Ii Vector At Xhoi Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+zap+ii/XhoI/pm34509026-86-5-11
Average 99 stars, based on 9085 article reviews
Lambda Zap Ii Vector At Xhoi Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+zap+ii/XhoI/pm34509026-86-5-11
Average 99 stars, based on 9085 article reviews
lambda zap ii vector at xhoi neb - by Bioz Stars,
2026-10
99/100 stars
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In Vitro:Article Title: Mechanistic insights into Sindbis virus infection: noncapped genomic RNAs enhance the translation of capped genomic RNAs to promote viral infectivity Article Snippet: .. Linearized templates for in vitro transcription of vRNA were prepared by digesting 4 μg of the infectious clone plasmids with the Amplification:Article Title: Enzymatic mutant suitable for homogeneous immunoassay method Article Snippet: This DNA encoded in a plasmid was amplified by PCR with reaction at 94° C. for 2 minutes followed by 30 cycles of reaction at 94° C. for 30 seconds, at 55° C. for 30 seconds, and at 68° C. for 1 minute by using the primers GUS_NotBack (ATAAGAATGCGGCCGCTATGTTACGTCCTGTAGAAA) (SEQ ID NO: 4) and GUSmIV5 XhoFor (GCCCTCGAGTCATTGTTTGTCTCCCTG) (SEQ ID NO: 5) and KOD-Plus-Neo DNA polymerase (TOYOBO CO., LTD.). .. The amplified GUS(IV-5)KE fragment was cleaved with the restriction enzymes NotI and Ligation:Article Title: Enzymatic mutant suitable for homogeneous immunoassay method Article Snippet: This DNA encoded in a plasmid was amplified by PCR with reaction at 94° C. for 2 minutes followed by 30 cycles of reaction at 94° C. for 30 seconds, at 55° C. for 30 seconds, and at 68° C. for 1 minute by using the primers GUS_NotBack (ATAAGAATGCGGCCGCTATGTTACGTCCTGTAGAAA) (SEQ ID NO: 4) and GUSmIV5 XhoFor (GCCCTCGAGTCATTGTTTGTCTCCCTG) (SEQ ID NO: 5) and KOD-Plus-Neo DNA polymerase (TOYOBO CO., LTD.). .. The amplified GUS(IV-5)KE fragment was cleaved with the restriction enzymes NotI and Purification:Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains. Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and Polymerase Chain Reaction:Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains. Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains. Article Snippet: PCR products were treated with DpnI to remove the parental plasmid before being transformed into DH5α-competent cells (Invitrogen). pcDNA4-hMMRN1 N-terminal EMI-Myc-His6 encoding the first 290 amino acids covering the N-terminal EMI domain of hMMRN1 was subcloned from pcDNA3.1-hMMRN1 WT-Myc-His6 using CloneAmp HiFi PCR Premix with primers AI364 and AI365. .. PCR products were digested with HindIII and Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection Article Snippet: .. The cloning strategy involved double-digestion of the pDZ plasmid with E co RI-HF (NEB, R3101S) and Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection. Article Snippet: .. The cloning strategy involved doubledigestion of the pDZ plasmid with EcoRI-HF (NEB, R3101S) and Clone Assay:Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains. Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains. Article Snippet: PCR products were treated with DpnI to remove the parental plasmid before being transformed into DH5α-competent cells (Invitrogen). pcDNA4-hMMRN1 N-terminal EMI-Myc-His6 encoding the first 290 amino acids covering the N-terminal EMI domain of hMMRN1 was subcloned from pcDNA3.1-hMMRN1 WT-Myc-His6 using CloneAmp HiFi PCR Premix with primers AI364 and AI365. .. PCR products were digested with HindIII and Expressing:Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains. Article Snippet: PCR products were treated with DpnI to remove the parental plasmid before being transformed into DH5α-competent cells (Invitrogen). pcDNA4-hMMRN1 N-terminal EMI-Myc-His6 encoding the first 290 amino acids covering the N-terminal EMI domain of hMMRN1 was subcloned from pcDNA3.1-hMMRN1 WT-Myc-His6 using CloneAmp HiFi PCR Premix with primers AI364 and AI365. .. PCR products were digested with HindIII and Cloning:Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection Article Snippet: .. The cloning strategy involved double-digestion of the pDZ plasmid with E co RI-HF (NEB, R3101S) and Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection. Article Snippet: .. The cloning strategy involved doubledigestion of the pDZ plasmid with EcoRI-HF (NEB, R3101S) and Plasmid Preparation:Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection Article Snippet: .. The cloning strategy involved double-digestion of the pDZ plasmid with E co RI-HF (NEB, R3101S) and Article Title: Context-dependent translation inhibition as a novel oncology therapeutic modality Article Snippet: .. The plasmid pT7CFE1 (Thermo Scientific) was digested in rCutsmart buffer with the Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection. Article Snippet: .. The cloning strategy involved doubledigestion of the pDZ plasmid with EcoRI-HF (NEB, R3101S) and |