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lambda zap ii vector at xhoi neb  (New England Biolabs)


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    Structured Review

    New England Biolabs lambda zap ii vector at xhoi neb
    Lambda Zap Ii Vector At Xhoi Neb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+zap+ii/XhoI/pm34509026-86-5-11
    Average 99 stars, based on 9085 article reviews
    lambda zap ii vector at xhoi neb - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: Mechanistic insights into Sindbis virus infection: noncapped genomic RNAs enhance the translation of capped genomic RNAs to promote viral infectivity
    Article Snippet: .. Linearized templates for in vitro transcription of vRNA were prepared by digesting 4 μg of the infectious clone plasmids with the restriction enzyme XhoI (New England Biolabs). ..

    Amplification:

    Article Title: Enzymatic mutant suitable for homogeneous immunoassay method
    Article Snippet: This DNA encoded in a plasmid was amplified by PCR with reaction at 94° C. for 2 minutes followed by 30 cycles of reaction at 94° C. for 30 seconds, at 55° C. for 30 seconds, and at 68° C. for 1 minute by using the primers GUS_NotBack (ATAAGAATGCGGCCGCTATGTTACGTCCTGTAGAAA) (SEQ ID NO: 4) and GUSmIV5 XhoFor (GCCCTCGAGTCATTGTTTGTCTCCCTG) (SEQ ID NO: 5) and KOD-Plus-Neo DNA polymerase (TOYOBO CO., LTD.). .. The amplified GUS(IV-5)KE fragment was cleaved with the restriction enzymes NotI and XhoI (New England Biolabs Japan, Tokyo), and inserted through ligation into the GUSm moiety of each of pET32-VH(NP)-GUSm and pET32-VL(NP)-GUSm (Su et al., Analyst, 143, 2096-101, 2018) cleaved with the same two restriction enzymes, and their sequences were confirmed. ..

    Ligation:

    Article Title: Enzymatic mutant suitable for homogeneous immunoassay method
    Article Snippet: This DNA encoded in a plasmid was amplified by PCR with reaction at 94° C. for 2 minutes followed by 30 cycles of reaction at 94° C. for 30 seconds, at 55° C. for 30 seconds, and at 68° C. for 1 minute by using the primers GUS_NotBack (ATAAGAATGCGGCCGCTATGTTACGTCCTGTAGAAA) (SEQ ID NO: 4) and GUSmIV5 XhoFor (GCCCTCGAGTCATTGTTTGTCTCCCTG) (SEQ ID NO: 5) and KOD-Plus-Neo DNA polymerase (TOYOBO CO., LTD.). .. The amplified GUS(IV-5)KE fragment was cleaved with the restriction enzymes NotI and XhoI (New England Biolabs Japan, Tokyo), and inserted through ligation into the GUSm moiety of each of pET32-VH(NP)-GUSm and pET32-VL(NP)-GUSm (Su et al., Analyst, 143, 2096-101, 2018) cleaved with the same two restriction enzymes, and their sequences were confirmed. ..

    Purification:

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and XhoI (NEB), ligated with T4 Ligase (NEB) and cloned into pcDNA4/TO/Myc-His6. pcDNA4-hEMID1 N-terminal EMI-Myc-His6, encoding the first 102 amino acids comprising the N-terminal portion and EMI, was subcloned from full-length pcDNA3.1-hEMID1-FLAG (made by gene synthesis, GenScript) as template. ..

    Polymerase Chain Reaction:

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and XhoI (NEB), ligated with T4 Ligase (NEB) and cloned into pcDNA4/TO/Myc-His6. pcDNA4-hEMID1 N-terminal EMI-Myc-His6, encoding the first 102 amino acids comprising the N-terminal portion and EMI, was subcloned from full-length pcDNA3.1-hEMID1-FLAG (made by gene synthesis, GenScript) as template. ..

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: PCR products were treated with DpnI to remove the parental plasmid before being transformed into DH5α-competent cells (Invitrogen). pcDNA4-hMMRN1 N-terminal EMI-Myc-His6 encoding the first 290 amino acids covering the N-terminal EMI domain of hMMRN1 was subcloned from pcDNA3.1-hMMRN1 WT-Myc-His6 using CloneAmp HiFi PCR Premix with primers AI364 and AI365. .. PCR products were digested with HindIII and XhoI and then ligated to pcDNA4/TO/Myc-His6 expression vector using T4 Polynucleotide Ligase (New England Biolabs (NEB)). pcDNA4-hMMRN2 N-terminal EMI-Myc-His6, encoding the first 132 amino acids corresponding to the N-terminal portion and EMI domain, was subcloned from full-length pcDNA3.1-hMMRN2-FLAG (made by gene synthesis, GenScript) as template, using cloned Pfu polymerase (VWR). .. Primers RU004 and RU005, synthesized by Invitrogen, were used.

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
    Article Snippet: .. The cloning strategy involved double-digestion of the pDZ plasmid with E co RI-HF (NEB, R3101S) and X ho I (NEB, R0146S), followed by In-Fusion cloning (Takara Bio, 638948) with three PCR-amplified fragments, containing the H7 ORF and its flanking regulatory regions. ..

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection.
    Article Snippet: .. The cloning strategy involved doubledigestion of the pDZ plasmid with EcoRI-HF (NEB, R3101S) and XhoI (NEB, R0146S), followed by In-Fusion cloning (Takara Bio, 638948) with three PCR-amplified fragments, containing the H7 ORF and its flanking regulatory regions. ..

    Clone Assay:

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and XhoI (NEB), ligated with T4 Ligase (NEB) and cloned into pcDNA4/TO/Myc-His6. pcDNA4-hEMID1 N-terminal EMI-Myc-His6, encoding the first 102 amino acids comprising the N-terminal portion and EMI, was subcloned from full-length pcDNA3.1-hEMID1-FLAG (made by gene synthesis, GenScript) as template. ..

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: PCR products were treated with DpnI to remove the parental plasmid before being transformed into DH5α-competent cells (Invitrogen). pcDNA4-hMMRN1 N-terminal EMI-Myc-His6 encoding the first 290 amino acids covering the N-terminal EMI domain of hMMRN1 was subcloned from pcDNA3.1-hMMRN1 WT-Myc-His6 using CloneAmp HiFi PCR Premix with primers AI364 and AI365. .. PCR products were digested with HindIII and XhoI and then ligated to pcDNA4/TO/Myc-His6 expression vector using T4 Polynucleotide Ligase (New England Biolabs (NEB)). pcDNA4-hMMRN2 N-terminal EMI-Myc-His6, encoding the first 132 amino acids corresponding to the N-terminal portion and EMI domain, was subcloned from full-length pcDNA3.1-hMMRN2-FLAG (made by gene synthesis, GenScript) as template, using cloned Pfu polymerase (VWR). .. Primers RU004 and RU005, synthesized by Invitrogen, were used.

    Expressing:

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: PCR products were treated with DpnI to remove the parental plasmid before being transformed into DH5α-competent cells (Invitrogen). pcDNA4-hMMRN1 N-terminal EMI-Myc-His6 encoding the first 290 amino acids covering the N-terminal EMI domain of hMMRN1 was subcloned from pcDNA3.1-hMMRN1 WT-Myc-His6 using CloneAmp HiFi PCR Premix with primers AI364 and AI365. .. PCR products were digested with HindIII and XhoI and then ligated to pcDNA4/TO/Myc-His6 expression vector using T4 Polynucleotide Ligase (New England Biolabs (NEB)). pcDNA4-hMMRN2 N-terminal EMI-Myc-His6, encoding the first 132 amino acids corresponding to the N-terminal portion and EMI domain, was subcloned from full-length pcDNA3.1-hMMRN2-FLAG (made by gene synthesis, GenScript) as template, using cloned Pfu polymerase (VWR). .. Primers RU004 and RU005, synthesized by Invitrogen, were used.

    Cloning:

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
    Article Snippet: .. The cloning strategy involved double-digestion of the pDZ plasmid with E co RI-HF (NEB, R3101S) and X ho I (NEB, R0146S), followed by In-Fusion cloning (Takara Bio, 638948) with three PCR-amplified fragments, containing the H7 ORF and its flanking regulatory regions. ..

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection.
    Article Snippet: .. The cloning strategy involved doubledigestion of the pDZ plasmid with EcoRI-HF (NEB, R3101S) and XhoI (NEB, R0146S), followed by In-Fusion cloning (Takara Bio, 638948) with three PCR-amplified fragments, containing the H7 ORF and its flanking regulatory regions. ..

    Plasmid Preparation:

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
    Article Snippet: .. The cloning strategy involved double-digestion of the pDZ plasmid with E co RI-HF (NEB, R3101S) and X ho I (NEB, R0146S), followed by In-Fusion cloning (Takara Bio, 638948) with three PCR-amplified fragments, containing the H7 ORF and its flanking regulatory regions. ..

    Article Title: Context-dependent translation inhibition as a novel oncology therapeutic modality
    Article Snippet: .. The plasmid pT7CFE1 (Thermo Scientific) was digested in rCutsmart buffer with the restriction enzymes XhoI and MscI (New England Biolabs) for 1 h at 37 °C. .. The linearized plasmid was used as a template for Gibson Assembly together with a gBlock (Integrated DNA Technologies) containing the coding sequence of interest and the HiBit tag, followed by a stop codon.

    Article Title: Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection.
    Article Snippet: .. The cloning strategy involved doubledigestion of the pDZ plasmid with EcoRI-HF (NEB, R3101S) and XhoI (NEB, R0146S), followed by In-Fusion cloning (Takara Bio, 638948) with three PCR-amplified fragments, containing the H7 ORF and its flanking regulatory regions. ..



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