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lambda zap ii phage vector  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda zap ii phage vector
    Lambda Zap Ii Phage Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+zap+ii/us11778965-890-20-35
    Average 90 stars, based on 1 article reviews
    lambda zap ii phage vector - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: High affinity human antibodies and human antibodies against human antigens
    Article Snippet: A 4 kb HindIII fragment of phage clone λ29.4, containing a γ switch region, was used to probe a human placenta genomic DNA library cloned into the phage vector lambda FIX™ II (Stratagene, La Jolla, Calif.).

    Article Title: Transgenic non-human animals for producing chimeric antibodies
    Article Snippet: A 4 kb HindIII fragment of phage clone λ29.4, containing a γ switch region, was used to probe a human placenta genomic DNA library cloned into the phage vector lambda FIXTM II (Stratagene, La Jolla, Calif.).

    Isolation:

    Article Title: Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
    Article Snippet: To enhance the nicotine to nornicotine conversion in the transgenic plants generated in this study, detached leaves were dipped into a solution of 0.2% ethephon, dried, and cured in plastic storage bags for seven days at room temperature in the dark. cDNA Libraries and Expressed Sequence Tags Total cellular RNA was isolated from senescing leaf tissue of Burley lines DH 91-1307-46(NC) and DH 91-1307-46(Con) using the TRIzol® reagent according to the manufacturer's protocol (Invitrogen). .. PolyA+RNA was isolated from total RNA using the MessageMaker system (Invitrogen), and cDNA was subsequently synthesized and cloned into the lambda ZAP II phage vector using the ZAP-cDNA Synthesis and Gigapack III Gold Cloning Kit (Stratagene). .. Aliquots of the phage libraries were converted to pBluescript-based plasmid libraries following the mass excision protocol outlined by Stratagene.

    Synthesized:

    Article Title: Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
    Article Snippet: To enhance the nicotine to nornicotine conversion in the transgenic plants generated in this study, detached leaves were dipped into a solution of 0.2% ethephon, dried, and cured in plastic storage bags for seven days at room temperature in the dark. cDNA Libraries and Expressed Sequence Tags Total cellular RNA was isolated from senescing leaf tissue of Burley lines DH 91-1307-46(NC) and DH 91-1307-46(Con) using the TRIzol® reagent according to the manufacturer's protocol (Invitrogen). .. PolyA+RNA was isolated from total RNA using the MessageMaker system (Invitrogen), and cDNA was subsequently synthesized and cloned into the lambda ZAP II phage vector using the ZAP-cDNA Synthesis and Gigapack III Gold Cloning Kit (Stratagene). .. Aliquots of the phage libraries were converted to pBluescript-based plasmid libraries following the mass excision protocol outlined by Stratagene.

    Clone Assay:

    Article Title: Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
    Article Snippet: To enhance the nicotine to nornicotine conversion in the transgenic plants generated in this study, detached leaves were dipped into a solution of 0.2% ethephon, dried, and cured in plastic storage bags for seven days at room temperature in the dark. cDNA Libraries and Expressed Sequence Tags Total cellular RNA was isolated from senescing leaf tissue of Burley lines DH 91-1307-46(NC) and DH 91-1307-46(Con) using the TRIzol® reagent according to the manufacturer's protocol (Invitrogen). .. PolyA+RNA was isolated from total RNA using the MessageMaker system (Invitrogen), and cDNA was subsequently synthesized and cloned into the lambda ZAP II phage vector using the ZAP-cDNA Synthesis and Gigapack III Gold Cloning Kit (Stratagene). .. Aliquots of the phage libraries were converted to pBluescript-based plasmid libraries following the mass excision protocol outlined by Stratagene.

    Cloning:

    Article Title: Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
    Article Snippet: To enhance the nicotine to nornicotine conversion in the transgenic plants generated in this study, detached leaves were dipped into a solution of 0.2% ethephon, dried, and cured in plastic storage bags for seven days at room temperature in the dark. cDNA Libraries and Expressed Sequence Tags Total cellular RNA was isolated from senescing leaf tissue of Burley lines DH 91-1307-46(NC) and DH 91-1307-46(Con) using the TRIzol® reagent according to the manufacturer's protocol (Invitrogen). .. PolyA+RNA was isolated from total RNA using the MessageMaker system (Invitrogen), and cDNA was subsequently synthesized and cloned into the lambda ZAP II phage vector using the ZAP-cDNA Synthesis and Gigapack III Gold Cloning Kit (Stratagene). .. Aliquots of the phage libraries were converted to pBluescript-based plasmid libraries following the mass excision protocol outlined by Stratagene.

    cDNA Synthesis:

    Article Title: Toxoplasma gondii cylclic GMP dependent protein kinase G as a chemotherapeutic target for antiprotozoal agents
    Article Snippet: Poly (A) RNA was twice selected using oligo (dT) cellulose chromatography and used as a template for first strand cDNA synthesis with SuperScript reverse transcriptase (Life Technologies, Inc.) and primed with oligo (dT). .. Following second strand cDNA synthesis and EcoR I adapter ligation, double stranded cDNA was size fractionated by column chromatography using Sephacryl S-500 HR and then ligated into the phage vector lambda ZAP II (Stratagene). .. Ligation reaction products were then packaged using Gigapack III Gold packaging extract (Stratagene) according to the manufacturer's recommendations.

    Article Title: Cyclic GMP dependent protein kinase as a chemotherapeutic target for antiprotozoal agents
    Article Snippet: Poly (A) RNA was twice selected using oligo (dT) cellulose chromatography and used as a template for first strand cDNA synthesis with SuperScript reverse transcriptase (Life Technologies, Inc.) and primed with oligo (dT). .. Following second strand cDNA synthesis and EcoR I adapter ligation, double stranded cDNA was size fractionated by column chromatography using Sephacryl S-500 HR and then ligated into the phage vector lambda ZAP II (Stratagene). .. Ligation reaction products were then packaged using Gigapack III Gold packaging extract (Stratagene) according to the manufacturer's recommendations.

    Adapter Ligation:

    Article Title: Toxoplasma gondii cylclic GMP dependent protein kinase G as a chemotherapeutic target for antiprotozoal agents
    Article Snippet: Poly (A) RNA was twice selected using oligo (dT) cellulose chromatography and used as a template for first strand cDNA synthesis with SuperScript reverse transcriptase (Life Technologies, Inc.) and primed with oligo (dT). .. Following second strand cDNA synthesis and EcoR I adapter ligation, double stranded cDNA was size fractionated by column chromatography using Sephacryl S-500 HR and then ligated into the phage vector lambda ZAP II (Stratagene). .. Ligation reaction products were then packaged using Gigapack III Gold packaging extract (Stratagene) according to the manufacturer's recommendations.

    Article Title: Cyclic GMP dependent protein kinase as a chemotherapeutic target for antiprotozoal agents
    Article Snippet: Poly (A) RNA was twice selected using oligo (dT) cellulose chromatography and used as a template for first strand cDNA synthesis with SuperScript reverse transcriptase (Life Technologies, Inc.) and primed with oligo (dT). .. Following second strand cDNA synthesis and EcoR I adapter ligation, double stranded cDNA was size fractionated by column chromatography using Sephacryl S-500 HR and then ligated into the phage vector lambda ZAP II (Stratagene). .. Ligation reaction products were then packaged using Gigapack III Gold packaging extract (Stratagene) according to the manufacturer's recommendations.

    Column Chromatography:

    Article Title: Toxoplasma gondii cylclic GMP dependent protein kinase G as a chemotherapeutic target for antiprotozoal agents
    Article Snippet: Poly (A) RNA was twice selected using oligo (dT) cellulose chromatography and used as a template for first strand cDNA synthesis with SuperScript reverse transcriptase (Life Technologies, Inc.) and primed with oligo (dT). .. Following second strand cDNA synthesis and EcoR I adapter ligation, double stranded cDNA was size fractionated by column chromatography using Sephacryl S-500 HR and then ligated into the phage vector lambda ZAP II (Stratagene). .. Ligation reaction products were then packaged using Gigapack III Gold packaging extract (Stratagene) according to the manufacturer's recommendations.

    Article Title: Cyclic GMP dependent protein kinase as a chemotherapeutic target for antiprotozoal agents
    Article Snippet: Poly (A) RNA was twice selected using oligo (dT) cellulose chromatography and used as a template for first strand cDNA synthesis with SuperScript reverse transcriptase (Life Technologies, Inc.) and primed with oligo (dT). .. Following second strand cDNA synthesis and EcoR I adapter ligation, double stranded cDNA was size fractionated by column chromatography using Sephacryl S-500 HR and then ligated into the phage vector lambda ZAP II (Stratagene). .. Ligation reaction products were then packaged using Gigapack III Gold packaging extract (Stratagene) according to the manufacturer's recommendations.

    Plasmid Preparation:

    Article Title: Toxoplasma gondii cylclic GMP dependent protein kinase G as a chemotherapeutic target for antiprotozoal agents
    Article Snippet: Poly (A) RNA was twice selected using oligo (dT) cellulose chromatography and used as a template for first strand cDNA synthesis with SuperScript reverse transcriptase (Life Technologies, Inc.) and primed with oligo (dT). .. Following second strand cDNA synthesis and EcoR I adapter ligation, double stranded cDNA was size fractionated by column chromatography using Sephacryl S-500 HR and then ligated into the phage vector lambda ZAP II (Stratagene). .. Ligation reaction products were then packaged using Gigapack III Gold packaging extract (Stratagene) according to the manufacturer's recommendations.

    Article Title: Cyclic GMP dependent protein kinase as a chemotherapeutic target for antiprotozoal agents
    Article Snippet: Poly (A) RNA was twice selected using oligo (dT) cellulose chromatography and used as a template for first strand cDNA synthesis with SuperScript reverse transcriptase (Life Technologies, Inc.) and primed with oligo (dT). .. Following second strand cDNA synthesis and EcoR I adapter ligation, double stranded cDNA was size fractionated by column chromatography using Sephacryl S-500 HR and then ligated into the phage vector lambda ZAP II (Stratagene). .. Ligation reaction products were then packaged using Gigapack III Gold packaging extract (Stratagene) according to the manufacturer's recommendations.



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