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Promega unmethylated lambda dna
Unmethylated Lambda Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+vector+dna/unmethylated+lambda+dna/pm38402612-186-34-42
Average 90 stars, based on 1 article reviews
unmethylated lambda dna - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Lambda DNA Preparation:


Article Title: Complementary genetic and epigenetic changes facilitate rapid adaptation to multiple global change stressors.
Article Snippet: For each sample, 250 ng of high- quality genomic DNA was digested with the restriction enzyme Msp1, 3’ ends were repaired with adenine, and Illumina methylated sequence adapters for NEBNext Ultra library preparation were ligated (New England Biolabs, Ipswich, MA). .. To quantify bisulfite conversion efficiency, 1 ng unmethylated lambda DNA (Promega) was added to each sample. ..

Article Title: DNA hypomethylation ameliorates erosive inflammatory arthritis by modulating interferon regulatory factor-8
Article Snippet: Genomic DNA (gDNA) was quantified using the Qubit Flex or the VarioSkan Flash (Qubit 1x HS dsDNA, Invitrogen, Cat# Q33231). .. 100ng of gDNA, including 0.2% Unmethylated Lambda DNA (Promega, Cat# D1521) was fragmented in a final volume of 50uL using the Covaris LE220 targeting ~350bp inserts. .. The fragmented gDNA was bisulfite converted with the EZ-96 DNA Methylation-Gold Mag Prep Kit (Zymo Research, Cat # D5043) according to the manufacturer’s recommendations.

Article Title: DNA methylation protects cancer cells against senescence.
Article Snippet: .. Briefly, 100 ng of genomic DNA was spiked with 1% (w/w) unmethylated lambda DNA (Promega) as a control for bisulfite conversion efficiency. .. The libraries were sequenced by Macrogen Japan Inc. on the Illumina HiSeq X Ten system.



other:

Article Title: DNA methylation memory of pancreatic acinar-ductal metaplasia transition state altering Kras-downstream PI3K and Rho GTPase signaling in the absence of Kras mutation.
Article Snippet: 1% unmethylated Lambda DNA (Promega cat. #D1521) was spiked into genomic DNA to monitor bisulfite conversion efficiency. xGen Methyl-Seq DNA Library Prep Kit (IDT, cat. #10009860) was used for WGBS library preparation.

Article Title: Integrative Molecular and Functional Analysis of Human Sperm Subpopulations to Identify New Biomarkers of Fertilization Potential.
Article Snippet: a Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico b Fundación Tambre, Madrid, Spain c Center for Reproductive Sciences, Department of Obstetrics and Gynecology, University of California School of Medicine, San Francisco, CA, USA d Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal e Weill Institute for Neurosciences, Department of Neurological Surgery, University of California San Francisco School of Medicine, San Francisco, CA, USA f Department of Genetics, The McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, USA g Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada h Department of Life Sciences, University of Coimbra, Coimbra, Portugal

Control:

Article Title: DNA methylation protects cancer cells against senescence.
Article Snippet: .. Briefly, 100 ng of genomic DNA was spiked with 1% (w/w) unmethylated lambda DNA (Promega) as a control for bisulfite conversion efficiency. .. The libraries were sequenced by Macrogen Japan Inc. on the Illumina HiSeq X Ten system.



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Image Search Results


Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

doi: 10.3389/fcimb.2018.00235

Figure Lengend Snippet: Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

Article Snippet: Herring sperm DNA (10164142), phage lambda DNA (D3779), and pBR322 (N3033L) DNA were purchased from Invitrogen (Karlsruhe, Germany), Sigma-Aldrich, and New England Biolabs (Frankfurt, Germany), respectively.

Techniques: Binding Assay, Incubation, Imaging, Lambda DNA Preparation, Plasmid Preparation, Isolation