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n3041s cpg methylated puc19 dna  (New England Biolabs)


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    New England Biolabs n3041s cpg methylated puc19 dna
    N3041s Cpg Methylated Puc19 Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+vector+dna/pUC19+Vector/pm38402612-186-61-59
    Average 97 stars, based on 1414 article reviews
    n3041s cpg methylated puc19 dna - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: How to convert a 3D printer to a personal automated liquid handler for life science workflows.
    Article Snippet: .. The end-point PCR and purification workflow used 10 ng of pUC19 vector (New England Biolabs, Ipswich, MA) as the input template, 200 nM forward primer 5’-AGG AGA AAA TAC CGC ATC AG-3’ for the 406 bp amplicon, 200 nM forward primer 5’-GAT CCT CTA GAG TCG ACC TG-3’ for the 206 bp amplicon, 200 nM universal reverse primer 5’-CAG GTT TCC CGA CTG GAA AG-3’ (Integrated DNA Technologies, Coralville, IA), and 1X Hot Start Taq Master Mix 50 μL reaction (New England Biolabs) in 200 μL reaction tubes (Eppendorf, Hamburg, Germany). .. The amplification followed a thermal cycle of 95 °C for 60 seconds as the initial denaturation, followed by 30 cycles of 95 °C for 15 seconds, 50 °C for 30 seconds, 68 °C for 30 seconds, and ending with the final extension of 62 °C for 300 seconds using Mastercycler nexus X2 (Eppendorf).

    Article Title: The Campylobacter jejuni BumS sensor phosphatase detects the branched short-chain fatty acids isobutyrate and isovalerate as direct cues for signal transduction.
    Article Snippet: Two-component signal transduction systems (TCSs) are nearly ubiquitous across bacterial species and enable bacteria to sense and respond to specific cues for environmental adaptation.. The Campylobacter jejuni BumSR TCS is unusual in that the BumS sensor exclusively functions as a phosphatase rather than a kinase to control phosphorylated levels of its cognate BumR response regulator (P-BumR).. We previously found that BumSR directs a response to the short-chain fatty acid butyrate generated by resident microbiota so that C. jejuni identifies ideal lower intestinal niches in avian and human hosts for colonization.

    Purification:

    Article Title: How to convert a 3D printer to a personal automated liquid handler for life science workflows.
    Article Snippet: .. The end-point PCR and purification workflow used 10 ng of pUC19 vector (New England Biolabs, Ipswich, MA) as the input template, 200 nM forward primer 5’-AGG AGA AAA TAC CGC ATC AG-3’ for the 406 bp amplicon, 200 nM forward primer 5’-GAT CCT CTA GAG TCG ACC TG-3’ for the 206 bp amplicon, 200 nM universal reverse primer 5’-CAG GTT TCC CGA CTG GAA AG-3’ (Integrated DNA Technologies, Coralville, IA), and 1X Hot Start Taq Master Mix 50 μL reaction (New England Biolabs) in 200 μL reaction tubes (Eppendorf, Hamburg, Germany). .. The amplification followed a thermal cycle of 95 °C for 60 seconds as the initial denaturation, followed by 30 cycles of 95 °C for 15 seconds, 50 °C for 30 seconds, 68 °C for 30 seconds, and ending with the final extension of 62 °C for 300 seconds using Mastercycler nexus X2 (Eppendorf).

    Amplification:

    Article Title: How to convert a 3D printer to a personal automated liquid handler for life science workflows.
    Article Snippet: .. The end-point PCR and purification workflow used 10 ng of pUC19 vector (New England Biolabs, Ipswich, MA) as the input template, 200 nM forward primer 5’-AGG AGA AAA TAC CGC ATC AG-3’ for the 406 bp amplicon, 200 nM forward primer 5’-GAT CCT CTA GAG TCG ACC TG-3’ for the 206 bp amplicon, 200 nM universal reverse primer 5’-CAG GTT TCC CGA CTG GAA AG-3’ (Integrated DNA Technologies, Coralville, IA), and 1X Hot Start Taq Master Mix 50 μL reaction (New England Biolabs) in 200 μL reaction tubes (Eppendorf, Hamburg, Germany). .. The amplification followed a thermal cycle of 95 °C for 60 seconds as the initial denaturation, followed by 30 cycles of 95 °C for 15 seconds, 50 °C for 30 seconds, 68 °C for 30 seconds, and ending with the final extension of 62 °C for 300 seconds using Mastercycler nexus X2 (Eppendorf).

    Article Title: Functional evaluation and clinical classification of BRCA2 variants.
    Article Snippet: Multiple sgRNAs were designed using the Benchling design tool. sgRNA-annealed oligonucleotides were ligated into pSpCas9(BB)-2A-Puro (PX459 v.2.0) (Addgene, 62988) following BbsI (New England Biolabs, R0539L) digestion to create a Cas9–sgRNA co-expression construct for each individual SGE. .. For each SGE, 600−1,000 bp homologous arms upstream and downstream of the target region were amplified from wild-type HAP1 gDNA and cloned into a BamHI-HF-digested pUC19 vector using a NEBuilder HiFi DNA assembly Cloning kit. ..

    Plasmid Preparation:

    Article Title: Promoter polynucleotide, signal polypeptide and use thereof
    Article Snippet: .. The pMT48 vector was a vector in which Rep gene (SEQ ID NO: 7) which is an origin of replication of a plasmid pLMT1-74 was introduced into the EcoRI site of pUC19 (New England Biolabs). ..

    Clone Assay:

    Article Title: Functional evaluation and clinical classification of BRCA2 variants.
    Article Snippet: Multiple sgRNAs were designed using the Benchling design tool. sgRNA-annealed oligonucleotides were ligated into pSpCas9(BB)-2A-Puro (PX459 v.2.0) (Addgene, 62988) following BbsI (New England Biolabs, R0539L) digestion to create a Cas9–sgRNA co-expression construct for each individual SGE. .. For each SGE, 600−1,000 bp homologous arms upstream and downstream of the target region were amplified from wild-type HAP1 gDNA and cloned into a BamHI-HF-digested pUC19 vector using a NEBuilder HiFi DNA assembly Cloning kit. ..

    Cloning:

    Article Title: Functional evaluation and clinical classification of BRCA2 variants.
    Article Snippet: Multiple sgRNAs were designed using the Benchling design tool. sgRNA-annealed oligonucleotides were ligated into pSpCas9(BB)-2A-Puro (PX459 v.2.0) (Addgene, 62988) following BbsI (New England Biolabs, R0539L) digestion to create a Cas9–sgRNA co-expression construct for each individual SGE. .. For each SGE, 600−1,000 bp homologous arms upstream and downstream of the target region were amplified from wild-type HAP1 gDNA and cloned into a BamHI-HF-digested pUC19 vector using a NEBuilder HiFi DNA assembly Cloning kit. ..

    Construct:

    Article Title: Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators
    Article Snippet: .. For lipofection, the construct was diluted 1:5 with an empty pUC19 vector (New England Biolabs) and then transfected with 7.5 μl of TransIT-293 and 2.5 μg of DNA. .. Cells were replated 36 to 60 hours after transfection on glass-bottom dishes (Cellvis, catalog no. D35-14-1.5-N) that were previously coated in poly- d -lysine to aid in cell adhesion.

    Transfection:

    Article Title: Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators
    Article Snippet: .. For lipofection, the construct was diluted 1:5 with an empty pUC19 vector (New England Biolabs) and then transfected with 7.5 μl of TransIT-293 and 2.5 μg of DNA. .. Cells were replated 36 to 60 hours after transfection on glass-bottom dishes (Cellvis, catalog no. D35-14-1.5-N) that were previously coated in poly- d -lysine to aid in cell adhesion.



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    Image Search Results


    Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation

    doi: 10.3389/fcimb.2018.00235

    Figure Lengend Snippet: Eap binding to DNA is affected by the state and type of DNA. Eap (0.5 μg/ml) was co-incubated with DNA samples (100 ng/ml) obtained from different sources, and subsequently attached to OTS silicon prior to AFM imaging. Representative AFM height images of Eap/DNA complexes adhering to OTS silicon from three independent experiments are shown: (A) Phage lambda DNA. (B) Artificial 1.4 kb PCR DNA-product. (C) Pst I-digested plasmid pBR322 (D) Circular pBR322 isolated from E. coli DH5α. (E) Sheared herring sperm DNA. Eap molecules putatively adhering to DNA (white arrows), OTS (yellow arrows), or cross-linking DNA (green arrows) are indicated. DNA molecules displayed in (A–C) , and (E) were aligned to OTS silicon by DNA combing.

    Article Snippet: Herring sperm DNA (10164142), phage lambda DNA (D3779), and pBR322 (N3033L) DNA were purchased from Invitrogen (Karlsruhe, Germany), Sigma-Aldrich, and New England Biolabs (Frankfurt, Germany), respectively.

    Techniques: Binding Assay, Incubation, Imaging, Lambda DNA Preparation, Plasmid Preparation, Isolation