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Promega lambda gt11 phage vector (huynh)
Lambda Gt11 Phage Vector (Huynh), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+phage+vector+zap+express/%CE%BBgt11/us07094406-559-4-14
Average 90 stars, based on 1 article reviews
lambda gt11 phage vector (huynh) - by Bioz Stars, 2026-09
90/100 stars

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other:

Article Title: Limited genetic divergence among Australian alpine Poa tussock grasses coupled with regional structuring points to ongoing gene flow and taxonomic challenges
Article Snippet: Initially, marker size was measured by comparison with the fmol ® λgt11 sequencing ladder (Promega).

Cloning:

Article Title: Complete sequence of the genes encoding the V H and V L regions of low- and high-affinity monoclonal IgM and lgA1 rheumatoid factors produced by CD5 + B cells from a rheumatoid arthritis patient
Article Snippet: Blots were hybridized with the different V H probes and washed as previously described ( 33 ) Autoradiography was performed using Kodak XAR-5 film (Eastman Kodak Co., Rochester, NY). .. Synthesis of cDNA, and cloning and sequencing of mAb V H and V L genes Total cDNA was synthesized from 5′ poly(A+) mRNA using ohgo(dT) priming and reagents as provided by Pharmacia LKB Biotechnology, Inc. cDNA with Eco RI adaptors on both sides was ligated into Eco RI-digested, dephosphorylated λ gt11 arms (Promega Biotec, Madison, Wl). .. Recombinant λ gt11 was packaged using the Packagene (Promega Biotec) packaging extract and used to construct recombinant libraries.

Sequencing:

Article Title: Complete sequence of the genes encoding the V H and V L regions of low- and high-affinity monoclonal IgM and lgA1 rheumatoid factors produced by CD5 + B cells from a rheumatoid arthritis patient
Article Snippet: Blots were hybridized with the different V H probes and washed as previously described ( 33 ) Autoradiography was performed using Kodak XAR-5 film (Eastman Kodak Co., Rochester, NY). .. Synthesis of cDNA, and cloning and sequencing of mAb V H and V L genes Total cDNA was synthesized from 5′ poly(A+) mRNA using ohgo(dT) priming and reagents as provided by Pharmacia LKB Biotechnology, Inc. cDNA with Eco RI adaptors on both sides was ligated into Eco RI-digested, dephosphorylated λ gt11 arms (Promega Biotec, Madison, Wl). .. Recombinant λ gt11 was packaged using the Packagene (Promega Biotec) packaging extract and used to construct recombinant libraries.

Article Title: Small population size and extremely low levels of genetic diversity in island populations of the platypus, Ornithorhynchus anatinus
Article Snippet: .. Alleles were sized by comparison with λgt11 ladders (Promega fmol_ DNA Cycle Sequencing System, Madison, WI). ..

Synthesized:

Article Title: Complete sequence of the genes encoding the V H and V L regions of low- and high-affinity monoclonal IgM and lgA1 rheumatoid factors produced by CD5 + B cells from a rheumatoid arthritis patient
Article Snippet: Blots were hybridized with the different V H probes and washed as previously described ( 33 ) Autoradiography was performed using Kodak XAR-5 film (Eastman Kodak Co., Rochester, NY). .. Synthesis of cDNA, and cloning and sequencing of mAb V H and V L genes Total cDNA was synthesized from 5′ poly(A+) mRNA using ohgo(dT) priming and reagents as provided by Pharmacia LKB Biotechnology, Inc. cDNA with Eco RI adaptors on both sides was ligated into Eco RI-digested, dephosphorylated λ gt11 arms (Promega Biotec, Madison, Wl). .. Recombinant λ gt11 was packaged using the Packagene (Promega Biotec) packaging extract and used to construct recombinant libraries.

Article Title: Complete sequence of the genes encoding the V H and V L regions of low- and high-affinity monoclonal IgM and lgA1 rheumatoid factors produced by CD5 + B cells from a rheumatoid arthritis patient
Article Snippet: Blots were hybridized with the different V H probes and washed as previously described ( 33 ) Autoradiography was performed using Kodak XAR-5 film (Eastman Kodak Co., Rochester, NY). .. Total cDNA was synthesized from 5′ poly(A+) mRNA using ohgo(dT) priming and reagents as provided by Pharmacia LKB Biotechnology, Inc. cDNA with Eco RI adaptors on both sides was ligated into Eco RI-digested, dephosphorylated λ gt11 arms (Promega Biotec, Madison, Wl). .. Recombinant λ gt11 was packaged using the Packagene (Promega Biotec) packaging extract and used to construct recombinant libraries.

Recombinant:

Article Title: Complete sequence of the genes encoding the V H and V L regions of low- and high-affinity monoclonal IgM and lgA1 rheumatoid factors produced by CD5 + B cells from a rheumatoid arthritis patient
Article Snippet: Synthesis of cDNA, and cloning and sequencing of mAb V H and V L genes Total cDNA was synthesized from 5′ poly(A+) mRNA using ohgo(dT) priming and reagents as provided by Pharmacia LKB Biotechnology, Inc. cDNA with Eco RI adaptors on both sides was ligated into Eco RI-digested, dephosphorylated λ gt11 arms (Promega Biotec, Madison, Wl). .. Recombinant λ gt11 was packaged using the Packagene (Promega Biotec) packaging extract and used to construct recombinant libraries. .. Replica filters (Gene Screen membranes, Biotechnology Systems NEN Research Products) from each plate were separately hybridized with appropriate 32 P-labeled H and L chain gene-specific DNA or oligonucleotide probes.

Construct:

Article Title: Complete sequence of the genes encoding the V H and V L regions of low- and high-affinity monoclonal IgM and lgA1 rheumatoid factors produced by CD5 + B cells from a rheumatoid arthritis patient
Article Snippet: Synthesis of cDNA, and cloning and sequencing of mAb V H and V L genes Total cDNA was synthesized from 5′ poly(A+) mRNA using ohgo(dT) priming and reagents as provided by Pharmacia LKB Biotechnology, Inc. cDNA with Eco RI adaptors on both sides was ligated into Eco RI-digested, dephosphorylated λ gt11 arms (Promega Biotec, Madison, Wl). .. Recombinant λ gt11 was packaged using the Packagene (Promega Biotec) packaging extract and used to construct recombinant libraries. .. Replica filters (Gene Screen membranes, Biotechnology Systems NEN Research Products) from each plate were separately hybridized with appropriate 32 P-labeled H and L chain gene-specific DNA or oligonucleotide probes.

Comparison:

Article Title: Small population size and extremely low levels of genetic diversity in island populations of the platypus, Ornithorhynchus anatinus
Article Snippet: .. Alleles were sized by comparison with λgt11 ladders (Promega fmol_ DNA Cycle Sequencing System, Madison, WI). ..



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