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lambda phage vector zap express  (Agilent technologies)


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    Agilent technologies lambda phage vector zap express
    Lambda Phage Vector Zap Express, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+phage+vector+zap+express/pmc00154039-70-30-35
    Average 90 stars, based on 1 article reviews
    lambda phage vector zap express - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: Structural integrity of the Golgi is temperature sensitive in conditional-lethal mutants with no detectable GM130.
    Article Snippet: At 39.5 C in the temperature-sensitive, conditional-lethal mutant ldlG, glycoprotein processing is disrupted and secretion is blocked.. The ultrastructure of the Golgi apparatus in ldlG cells was examined using immunofluorescence and immunoelectron microscopy.. At 34 C the structure of the Golgi apparatus was normal, whereas after incubation at 39.5 C for 12 h it disassembled into dispersed vesicles.

    Clone Assay:

    Article Title: Structural integrity of the Golgi is temperature sensitive in conditional-lethal mutants with no detectable GM130.
    Article Snippet: At 39.5 C in the temperature-sensitive, conditional-lethal mutant ldlG, glycoprotein processing is disrupted and secretion is blocked.. The ultrastructure of the Golgi apparatus in ldlG cells was examined using immunofluorescence and immunoelectron microscopy.. At 34 C the structure of the Golgi apparatus was normal, whereas after incubation at 39.5 C for 12 h it disassembled into dispersed vesicles.

    Article Title: Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.
    Article Snippet: In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., ‘G004’ and ‘Hakusai Chukanbohon Nou 7’ (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance.. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes.. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance.

    Construct:

    Article Title: Structural integrity of the Golgi is temperature sensitive in conditional-lethal mutants with no detectable GM130.
    Article Snippet: At 39.5 C in the temperature-sensitive, conditional-lethal mutant ldlG, glycoprotein processing is disrupted and secretion is blocked.. The ultrastructure of the Golgi apparatus in ldlG cells was examined using immunofluorescence and immunoelectron microscopy.. At 34 C the structure of the Golgi apparatus was normal, whereas after incubation at 39.5 C for 12 h it disassembled into dispersed vesicles.

    Article Title: Genomic cloning of feline Fas ligand gene and characterization of the transcription regulatory region.
    Article Snippet: The feline Fas ligand gene was molecularly cloned from a feline genomic library and its genomic organization was determined.. The feline Fas ligand gene contained four exons and spanned approximately 10 kb in the genome, and thus had the same structure as the human Fas ligand gene.. The promoter region of the feline Fas ligand gene was further characterized by deletion mapping.

    Article Title: Identification of genes involved in biosynthesis of Actinobacillus pleuropneumoniae serotype 1 O-antigen and biological properties of rough mutants
    Article Snippet: DNA sequence information was analyzed with software from the University of Wisconsin Genetic Computer Group20 using the National Center for Biotechnology Information (NCBI) databases. .. A DNA library of A. pleuropneumoniae serotype 1 reference strain 407421 constructed in Lambda Zap Express phage vector (Stratagene, La Jolla, CA, USA) was screened with DIG-labeled probes designed for Southern blotting. ..

    Article Title: Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.
    Article Snippet: In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., ‘G004’ and ‘Hakusai Chukanbohon Nou 7’ (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance.. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes.. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance.

    Article Title: Identification, cloning and characterization ofrfaEofActinobacillus pleuropneumoniaeserotype 1, a gene involved in lipopolysaccharide inner-core biosynthesis
    Article Snippet: Plasmid DNA was extracted with the Plasmid QIAprep spin miniprep kit (Qiagen Inc., Mississauga, ON, Canada). .. A plasmidic DNA library of A. pleuropneumoniae serotype 1 reference strain 4074 was constructed in Lambda Zap Express phage vector (Stratagene, La Jolla, CA, USA) and used for complementation assays. ..

    Article Title: Monoclonal Antibodies Bind A SNP-Sensitive Epitope that is Present Uniquely in Mycobacterium avium Subspecies Paratuberculosis
    Article Snippet: .. A MAP strain ATCC19698 expression library was constructed in the lambda ZAP phage vector (Agilent technologies-Stratagene, La Jolla, CA, USA) using size selected fragments in the 3–6 kb range as described previously (Bannantine and Stabel, ). ..

    Isolation:

    Article Title: Structural integrity of the Golgi is temperature sensitive in conditional-lethal mutants with no detectable GM130.
    Article Snippet: At 39.5 C in the temperature-sensitive, conditional-lethal mutant ldlG, glycoprotein processing is disrupted and secretion is blocked.. The ultrastructure of the Golgi apparatus in ldlG cells was examined using immunofluorescence and immunoelectron microscopy.. At 34 C the structure of the Golgi apparatus was normal, whereas after incubation at 39.5 C for 12 h it disassembled into dispersed vesicles.

    Article Title: Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.
    Article Snippet: In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., ‘G004’ and ‘Hakusai Chukanbohon Nou 7’ (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance.. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes.. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance.

    Article Title: Transcriptional Regulatory Properties of Epstein-Barr Virus Nuclear Antigen 3C Are Conserved in Simian Lymphocryptoviruses
    Article Snippet: .. Briefly, BaLCV-enriched DNA was isolated from S594 cells by using a modified Hirt procedure ( 42 ), digested with restriction enzymes, and used to generate a genomic library in the lambda phage vector ZAP Express (Stratagene). ..

    Modification:

    Article Title: Structural integrity of the Golgi is temperature sensitive in conditional-lethal mutants with no detectable GM130.
    Article Snippet: At 39.5 C in the temperature-sensitive, conditional-lethal mutant ldlG, glycoprotein processing is disrupted and secretion is blocked.. The ultrastructure of the Golgi apparatus in ldlG cells was examined using immunofluorescence and immunoelectron microscopy.. At 34 C the structure of the Golgi apparatus was normal, whereas after incubation at 39.5 C for 12 h it disassembled into dispersed vesicles.

    Article Title: Transcriptional Regulatory Properties of Epstein-Barr Virus Nuclear Antigen 3C Are Conserved in Simian Lymphocryptoviruses
    Article Snippet: .. Briefly, BaLCV-enriched DNA was isolated from S594 cells by using a modified Hirt procedure ( 42 ), digested with restriction enzymes, and used to generate a genomic library in the lambda phage vector ZAP Express (Stratagene). ..

    Plasmid Preparation:

    Article Title: Structural integrity of the Golgi is temperature sensitive in conditional-lethal mutants with no detectable GM130.
    Article Snippet: At 39.5 C in the temperature-sensitive, conditional-lethal mutant ldlG, glycoprotein processing is disrupted and secretion is blocked.. The ultrastructure of the Golgi apparatus in ldlG cells was examined using immunofluorescence and immunoelectron microscopy.. At 34 C the structure of the Golgi apparatus was normal, whereas after incubation at 39.5 C for 12 h it disassembled into dispersed vesicles.

    Article Title: Genomic cloning of feline Fas ligand gene and characterization of the transcription regulatory region.
    Article Snippet: The feline Fas ligand gene was molecularly cloned from a feline genomic library and its genomic organization was determined.. The feline Fas ligand gene contained four exons and spanned approximately 10 kb in the genome, and thus had the same structure as the human Fas ligand gene.. The promoter region of the feline Fas ligand gene was further characterized by deletion mapping.

    Article Title: Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.
    Article Snippet: In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., ‘G004’ and ‘Hakusai Chukanbohon Nou 7’ (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance.. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes.. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance.

    Article Title: Transcriptional Regulatory Properties of Epstein-Barr Virus Nuclear Antigen 3C Are Conserved in Simian Lymphocryptoviruses
    Article Snippet: .. Briefly, BaLCV-enriched DNA was isolated from S594 cells by using a modified Hirt procedure ( 42 ), digested with restriction enzymes, and used to generate a genomic library in the lambda phage vector ZAP Express (Stratagene). ..

    Article Title: Monoclonal Antibodies Bind A SNP-Sensitive Epitope that is Present Uniquely in Mycobacterium avium Subspecies Paratuberculosis
    Article Snippet: .. A MAP strain ATCC19698 expression library was constructed in the lambda ZAP phage vector (Agilent technologies-Stratagene, La Jolla, CA, USA) using size selected fragments in the 3–6 kb range as described previously (Bannantine and Stabel, ). ..

    Southern Blot:

    Article Title: Identification of genes involved in biosynthesis of Actinobacillus pleuropneumoniae serotype 1 O-antigen and biological properties of rough mutants
    Article Snippet: DNA sequence information was analyzed with software from the University of Wisconsin Genetic Computer Group20 using the National Center for Biotechnology Information (NCBI) databases. .. A DNA library of A. pleuropneumoniae serotype 1 reference strain 407421 constructed in Lambda Zap Express phage vector (Stratagene, La Jolla, CA, USA) was screened with DIG-labeled probes designed for Southern blotting. ..

    Hybridization:

    Article Title: Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L.
    Article Snippet: In an analysis of 114 F2 individuals from a cross between clubroot-resistant and susceptible lines of Brassica rapa L., ‘G004’ and ‘Hakusai Chukanbohon Nou 7’ (A9709), respectively, we identified two loci, Crr1 and Crr2, for clubroot (caused by Plasmodiophora brassicae Woronin) resistance.. Each locus segregated independently among the F2 population, indicating that the loci reside on a different region of chromosomes or on different chromosomes.. Genetic analysis showed that each locus had little effect on clubroot resistance by itself, indicating that these two loci are complementary for clubroot resistance.

    Expressing:

    Article Title: Monoclonal Antibodies Bind A SNP-Sensitive Epitope that is Present Uniquely in Mycobacterium avium Subspecies Paratuberculosis
    Article Snippet: .. A MAP strain ATCC19698 expression library was constructed in the lambda ZAP phage vector (Agilent technologies-Stratagene, La Jolla, CA, USA) using size selected fragments in the 3–6 kb range as described previously (Bannantine and Stabel, ). ..

    other:

    Article Title: Molecular cloning and characterization of multidomain xylanase from manure library
    Article Snippet: The gene (manf-x10) encoding xylanase from an environmental genomic DNA library was cloned and expressed in Escherichia coli.. The manf-x10 encoded a predicted protein of 467 amino acids residues with a molecular mass of 50.3 kD.. Sequence analysis of manf-x10 gene revealed that the N-terminus had high homology to the catalytic domain of other bacterial xylanase enzymes.



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