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goat anti λ  (SouthernBiotech)


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    Structured Review

    SouthernBiotech goat anti λ
    Goat Anti λ, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+phage+vector+zap+express/Goat+Anti-Human+Lambda-AP/pmc04106706-59-8-13
    Average 93 stars, based on 44 article reviews
    goat anti λ - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Enzyme-linked Immunospot:

    Article Title: Discovery and Evaluation of TLR-Targeted Immune Agonists.
    Article Snippet: Toll-like receptor (TLR) activation converts immunologically inactive tumors into immunologically active tumors by activating tumor residing antigen-presenting cells and recruitment of cytotoxic T lymphocytes.. Targeted immune agonists (TIAs) are antibody drug conjugates with small-molecule TLR agonist payloads.. The mechanism of action of TIAs involves tumor antigen recognition, Fcγ-receptor-dependent phagocytosis, and TLR-mediated activation to drive tumor killing by myeloid cells.

    Article Title: Development and application of an in vitro assay to assess target-independent B-cell activation by targeted TLR7 immune agonists.
    Article Snippet: .. Capture antibodies (a 1:1 mix of goat F(ab’)2 antihuman kappa and goat F(ab’)2 anti-human lambda; both from SouthernBiotech) were diluted each to final 5 μg/ml in PBS and incubated in ELISPOT plates overnight at 4 ◦C with seal. ..

    Flow Cytometry:

    Article Title: A mouse model of cardiac immunoglobulin light chain amyloidosis reveals insights into tissue accumulation and toxicity of amyloid fibrils
    Article Snippet: .. Table S2: cardiac and fibrosis-related DEG ALvsCtrl ALvsLS Log2 FC FDR Log2 FC FDR Nmrk2 3.91 0.0007 3.44 0.0062 Irf7 2.09 0.0033 3.45 0.0011 Gpnmb 4.12 1.85E-05 3.47 0.0001 Mmp12 7.6 0.0026 8.17 0.0098 Lgals3 2.7 0.0001 4.09 0.0002 Ltbp2 2.71 0.0029 3.52 0.0058 Timp1 2.63 0.0094 3.74 0.0129 Nppa 3.58 0.0021 1.992 0.095 Table S3: List of antibodies used in the study Antibody Reactivity Clone Source Conjugate Dilution Applications Anti-B220 Mouse Clone RA3-6B2 (Becton Dickinson #562922) Rat BV421 1/100 Flow cytometry Anti-CD138 Mouse Clone 281-2 (BD Biosciences #558626) Rat APC 1/100 Flow cytometry Anti-human VL6 Human Monoclonal (from A. Solomon’s lab) Mouse Unconjugated 1/200 Western-Blot Anti-lambda Human Polyclonal (southernBiotech #2070- 01, #2070-04) Goat Unconjugated or AP 1/1000 ELISA Anti-lambda Human Polyclonal (Dako #A0194) Goat Unconjugated, HRP, gold particles 1/1000 Western Blot Anti-lambda Human Polyclonal (Dako, #F0435) Rabbit (Fab'2) FITC 1/2000 Flow cytometry+ IF Anti-lambda Human Clone [4C2] (Abcam, #ab77275) Mouse FITC 1/50 IF Anti-IgG Rabbit Polyclonal (Beckman Coulter, #4050-05) Goat HRP 1/2000 Western-Blot Anti-IgG Mouse Polyclonal (Beckman Coulter #1031-05) Goat HRP 1/2000 Western-Blot AntiSAA1+SAA2 Mouse Polyclonal (Abcam, #ab199030) Rabbit Unconjugated 1/120 IF Anti-ApoA2 Mouse Polyclonal (AbClonal, #A14690) Rabbit Unconjugated 1/100 IF Anti-IgG Rabbit Polyclonal (Molecular probes #A21245) Goat AF647 1/100 IF Anti-SAP Mouse Clone 300103, (R&D Systems #MAB2556) Rat Unconjugated 1/100 IF Anti-IgG Rat Polyclonal (Molecular probes #A11081) Goat AF546 1/100 IF Supplemental figures ..

    Western Blot:

    Article Title: A mouse model of cardiac immunoglobulin light chain amyloidosis reveals insights into tissue accumulation and toxicity of amyloid fibrils
    Article Snippet: .. Table S2: cardiac and fibrosis-related DEG ALvsCtrl ALvsLS Log2 FC FDR Log2 FC FDR Nmrk2 3.91 0.0007 3.44 0.0062 Irf7 2.09 0.0033 3.45 0.0011 Gpnmb 4.12 1.85E-05 3.47 0.0001 Mmp12 7.6 0.0026 8.17 0.0098 Lgals3 2.7 0.0001 4.09 0.0002 Ltbp2 2.71 0.0029 3.52 0.0058 Timp1 2.63 0.0094 3.74 0.0129 Nppa 3.58 0.0021 1.992 0.095 Table S3: List of antibodies used in the study Antibody Reactivity Clone Source Conjugate Dilution Applications Anti-B220 Mouse Clone RA3-6B2 (Becton Dickinson #562922) Rat BV421 1/100 Flow cytometry Anti-CD138 Mouse Clone 281-2 (BD Biosciences #558626) Rat APC 1/100 Flow cytometry Anti-human VL6 Human Monoclonal (from A. Solomon’s lab) Mouse Unconjugated 1/200 Western-Blot Anti-lambda Human Polyclonal (southernBiotech #2070- 01, #2070-04) Goat Unconjugated or AP 1/1000 ELISA Anti-lambda Human Polyclonal (Dako #A0194) Goat Unconjugated, HRP, gold particles 1/1000 Western Blot Anti-lambda Human Polyclonal (Dako, #F0435) Rabbit (Fab'2) FITC 1/2000 Flow cytometry+ IF Anti-lambda Human Clone [4C2] (Abcam, #ab77275) Mouse FITC 1/50 IF Anti-IgG Rabbit Polyclonal (Beckman Coulter, #4050-05) Goat HRP 1/2000 Western-Blot Anti-IgG Mouse Polyclonal (Beckman Coulter #1031-05) Goat HRP 1/2000 Western-Blot AntiSAA1+SAA2 Mouse Polyclonal (Abcam, #ab199030) Rabbit Unconjugated 1/120 IF Anti-ApoA2 Mouse Polyclonal (AbClonal, #A14690) Rabbit Unconjugated 1/100 IF Anti-IgG Rabbit Polyclonal (Molecular probes #A21245) Goat AF647 1/100 IF Anti-SAP Mouse Clone 300103, (R&D Systems #MAB2556) Rat Unconjugated 1/100 IF Anti-IgG Rat Polyclonal (Molecular probes #A11081) Goat AF546 1/100 IF Supplemental figures ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: A mouse model of cardiac immunoglobulin light chain amyloidosis reveals insights into tissue accumulation and toxicity of amyloid fibrils
    Article Snippet: .. Table S2: cardiac and fibrosis-related DEG ALvsCtrl ALvsLS Log2 FC FDR Log2 FC FDR Nmrk2 3.91 0.0007 3.44 0.0062 Irf7 2.09 0.0033 3.45 0.0011 Gpnmb 4.12 1.85E-05 3.47 0.0001 Mmp12 7.6 0.0026 8.17 0.0098 Lgals3 2.7 0.0001 4.09 0.0002 Ltbp2 2.71 0.0029 3.52 0.0058 Timp1 2.63 0.0094 3.74 0.0129 Nppa 3.58 0.0021 1.992 0.095 Table S3: List of antibodies used in the study Antibody Reactivity Clone Source Conjugate Dilution Applications Anti-B220 Mouse Clone RA3-6B2 (Becton Dickinson #562922) Rat BV421 1/100 Flow cytometry Anti-CD138 Mouse Clone 281-2 (BD Biosciences #558626) Rat APC 1/100 Flow cytometry Anti-human VL6 Human Monoclonal (from A. Solomon’s lab) Mouse Unconjugated 1/200 Western-Blot Anti-lambda Human Polyclonal (southernBiotech #2070- 01, #2070-04) Goat Unconjugated or AP 1/1000 ELISA Anti-lambda Human Polyclonal (Dako #A0194) Goat Unconjugated, HRP, gold particles 1/1000 Western Blot Anti-lambda Human Polyclonal (Dako, #F0435) Rabbit (Fab'2) FITC 1/2000 Flow cytometry+ IF Anti-lambda Human Clone [4C2] (Abcam, #ab77275) Mouse FITC 1/50 IF Anti-IgG Rabbit Polyclonal (Beckman Coulter, #4050-05) Goat HRP 1/2000 Western-Blot Anti-IgG Mouse Polyclonal (Beckman Coulter #1031-05) Goat HRP 1/2000 Western-Blot AntiSAA1+SAA2 Mouse Polyclonal (Abcam, #ab199030) Rabbit Unconjugated 1/120 IF Anti-ApoA2 Mouse Polyclonal (AbClonal, #A14690) Rabbit Unconjugated 1/100 IF Anti-IgG Rabbit Polyclonal (Molecular probes #A21245) Goat AF647 1/100 IF Anti-SAP Mouse Clone 300103, (R&D Systems #MAB2556) Rat Unconjugated 1/100 IF Anti-IgG Rat Polyclonal (Molecular probes #A11081) Goat AF546 1/100 IF Supplemental figures ..

    Article Title: Novel and unique rheumatoid factors cross-react with viral epitopes in COVID-19.
    Article Snippet: Rheumatoid factors (RFs), polyreactive antibodies canonically known to bind two conformational epitopes of IgG Fc, are a hallmark of rheumatoid arthritis but also can arise in other inflammatory conditions and infections.. Also, infections may contribute to the development of rheumatoid arthritis and other autoimmune diseases.. Recently, RFs only in rheumatoid arthritis were found to bind novel linear IgG epitopes as well as thousands of other rheumatoid arthritis autoantigens.

    Magnetic Beads:

    Article Title: Novel and unique rheumatoid factors cross-react with viral epitopes in COVID-19.
    Article Snippet: Rheumatoid factors (RFs), polyreactive antibodies canonically known to bind two conformational epitopes of IgG Fc, are a hallmark of rheumatoid arthritis but also can arise in other inflammatory conditions and infections.. Also, infections may contribute to the development of rheumatoid arthritis and other autoimmune diseases.. Recently, RFs only in rheumatoid arthritis were found to bind novel linear IgG epitopes as well as thousands of other rheumatoid arthritis autoantigens.

    Incubation:

    Article Title: Development and application of an in vitro assay to assess target-independent B-cell activation by targeted TLR7 immune agonists.
    Article Snippet: .. Capture antibodies (a 1:1 mix of goat F(ab’)2 antihuman kappa and goat F(ab’)2 anti-human lambda; both from SouthernBiotech) were diluted each to final 5 μg/ml in PBS and incubated in ELISPOT plates overnight at 4 ◦C with seal. ..

    other:

    Article Title: A genome wide CRISPR screen reveals novel determinants of long-lived plasma cell secretory capacity
    Article Snippet: A similar workflow was used for detection of NP-specific antibodies in serum, except that ELISA plates were coated with NP 10-19 -BSA (Biosearch technologies) and detected with a Peroxidase-conjugated donkey anti-mouse IgG(H+L) (Jackson ImmunoResearch).



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