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embl3 sp6 t7 lambda bamh1 cloned library  (TaKaRa)


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    TaKaRa embl3 sp6 t7 lambda bamh1 cloned library
    Embl3 Sp6 T7 Lambda Bamh1 Cloned Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14661 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+embl3+sp6/BamH+I/us07375182-1653-6-12
    Average 99 stars, based on 14661 article reviews
    embl3 sp6 t7 lambda bamh1 cloned library - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: An ethylene-responsive element with spatial bias in Arabidopsis.
    Article Snippet: stress through a complex signal transduction network [1, 2].. Ethylene response factors (ERFs) are key downstream transcription factors in the ethylene signalling pathway.. A well-characterized DNA motif recognized by ERFs is the GCC box (5′A G C C G C C-3′), a canonical ethylene-responsive element [3].

    Article Title: An ancestral glutamate receptor mediates cell volume regulation during high K + stress in cyanobacteria
    Article Snippet: .. DNA sequences encoding GluR0 and KirBac6.1 were PCR amplified using Synechocystis genomic DNA as a template and cloned into the BamHI/SalI sites of pSTV28 (TaKaRa) to generate pSTV28-GluR0 and pSTV28-KirBac6.1, respectively. ..

    Clone Assay:

    Article Title: An ethylene-responsive element with spatial bias in Arabidopsis.
    Article Snippet: stress through a complex signal transduction network [1, 2].. Ethylene response factors (ERFs) are key downstream transcription factors in the ethylene signalling pathway.. A well-characterized DNA motif recognized by ERFs is the GCC box (5′A G C C G C C-3′), a canonical ethylene-responsive element [3].

    Article Title: Cancer-linked ANKRD26-RET uses an unusual combination of pathomechanisms leading to strongly increased cell proliferation.
    Article Snippet: .. Human ANKRD26-GFP was cloned by PCR using EST clone 6830677 (NM_014915.2) as template and subcloned into pEGFP-N3 (Clontech) using KpnI und BamHI as restriction sites, as described before [19]. ..

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Article Title: An ancestral glutamate receptor mediates cell volume regulation during high K + stress in cyanobacteria
    Article Snippet: .. DNA sequences encoding GluR0 and KirBac6.1 were PCR amplified using Synechocystis genomic DNA as a template and cloned into the BamHI/SalI sites of pSTV28 (TaKaRa) to generate pSTV28-GluR0 and pSTV28-KirBac6.1, respectively. ..

    Plasmid Preparation:

    Article Title: An ethylene-responsive element with spatial bias in Arabidopsis.
    Article Snippet: stress through a complex signal transduction network [1, 2].. Ethylene response factors (ERFs) are key downstream transcription factors in the ethylene signalling pathway.. A well-characterized DNA motif recognized by ERFs is the GCC box (5′A G C C G C C-3′), a canonical ethylene-responsive element [3].

    Article Title: Immunoglobulin G and immunoglobulin A anti-Ro52 in plasma and saliva show little association with disease activity in patients with primary Sjögren's disease.
    Article Snippet: .. Using the full-length TRIM21 gene as a template, four constructs for the expression of individual domains C-terminally fused to MBP were generated by polymerase chain reaction (PCR) and inserted into the NcoI and BamHI sites of the pET15b vector by In-Fusion cloning (Takara Bio, Kusatsu, Japan) (1): residues 1–85 encompassing the RING domain (2), residues 86–130 (B-Box) (3), residues 131–276 (Coiled–Coil) and (4) residues 277–475 (PRY/SPRY). .. To eliminate the inherent IgG (Fc) binding activity of the PRY/SPRY domain, the critical residues Trp 381 and Trp 383 were mutated to www.scandjrheumatol.se alanine by QuickChange (Agilent, Santa Clara, CA, USA) site-directed mutagenesis reactions.

    Article Title: CpxR and LrhA coordinate the regulation of Xenocoumacin 1 biosynthesis, flagellar assembly, and chemotaxis in Xenorhabdus nematophila
    Article Snippet: .. The pET28a vector was digested with restriction endonuclease BamH I and Nde I (both from Takara, Japan) to generate N-terminal 6 × His-tagged expression vectors, while digestion with Nco I and Xho I (both from Takara, Japan) was conducted to construct C-terminal 6 × His-tagged expression vectors. ..

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Construct:

    Article Title: Immunoglobulin G and immunoglobulin A anti-Ro52 in plasma and saliva show little association with disease activity in patients with primary Sjögren's disease.
    Article Snippet: .. Using the full-length TRIM21 gene as a template, four constructs for the expression of individual domains C-terminally fused to MBP were generated by polymerase chain reaction (PCR) and inserted into the NcoI and BamHI sites of the pET15b vector by In-Fusion cloning (Takara Bio, Kusatsu, Japan) (1): residues 1–85 encompassing the RING domain (2), residues 86–130 (B-Box) (3), residues 131–276 (Coiled–Coil) and (4) residues 277–475 (PRY/SPRY). .. To eliminate the inherent IgG (Fc) binding activity of the PRY/SPRY domain, the critical residues Trp 381 and Trp 383 were mutated to www.scandjrheumatol.se alanine by QuickChange (Agilent, Santa Clara, CA, USA) site-directed mutagenesis reactions.

    Article Title: CpxR and LrhA coordinate the regulation of Xenocoumacin 1 biosynthesis, flagellar assembly, and chemotaxis in Xenorhabdus nematophila
    Article Snippet: .. The pET28a vector was digested with restriction endonuclease BamH I and Nde I (both from Takara, Japan) to generate N-terminal 6 × His-tagged expression vectors, while digestion with Nco I and Xho I (both from Takara, Japan) was conducted to construct C-terminal 6 × His-tagged expression vectors. ..

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Expressing:

    Article Title: Immunoglobulin G and immunoglobulin A anti-Ro52 in plasma and saliva show little association with disease activity in patients with primary Sjögren's disease.
    Article Snippet: .. Using the full-length TRIM21 gene as a template, four constructs for the expression of individual domains C-terminally fused to MBP were generated by polymerase chain reaction (PCR) and inserted into the NcoI and BamHI sites of the pET15b vector by In-Fusion cloning (Takara Bio, Kusatsu, Japan) (1): residues 1–85 encompassing the RING domain (2), residues 86–130 (B-Box) (3), residues 131–276 (Coiled–Coil) and (4) residues 277–475 (PRY/SPRY). .. To eliminate the inherent IgG (Fc) binding activity of the PRY/SPRY domain, the critical residues Trp 381 and Trp 383 were mutated to www.scandjrheumatol.se alanine by QuickChange (Agilent, Santa Clara, CA, USA) site-directed mutagenesis reactions.

    Article Title: CpxR and LrhA coordinate the regulation of Xenocoumacin 1 biosynthesis, flagellar assembly, and chemotaxis in Xenorhabdus nematophila
    Article Snippet: .. The pET28a vector was digested with restriction endonuclease BamH I and Nde I (both from Takara, Japan) to generate N-terminal 6 × His-tagged expression vectors, while digestion with Nco I and Xho I (both from Takara, Japan) was conducted to construct C-terminal 6 × His-tagged expression vectors. ..

    Generated:

    Article Title: Immunoglobulin G and immunoglobulin A anti-Ro52 in plasma and saliva show little association with disease activity in patients with primary Sjögren's disease.
    Article Snippet: .. Using the full-length TRIM21 gene as a template, four constructs for the expression of individual domains C-terminally fused to MBP were generated by polymerase chain reaction (PCR) and inserted into the NcoI and BamHI sites of the pET15b vector by In-Fusion cloning (Takara Bio, Kusatsu, Japan) (1): residues 1–85 encompassing the RING domain (2), residues 86–130 (B-Box) (3), residues 131–276 (Coiled–Coil) and (4) residues 277–475 (PRY/SPRY). .. To eliminate the inherent IgG (Fc) binding activity of the PRY/SPRY domain, the critical residues Trp 381 and Trp 383 were mutated to www.scandjrheumatol.se alanine by QuickChange (Agilent, Santa Clara, CA, USA) site-directed mutagenesis reactions.

    Polymerase Chain Reaction:

    Article Title: Immunoglobulin G and immunoglobulin A anti-Ro52 in plasma and saliva show little association with disease activity in patients with primary Sjögren's disease.
    Article Snippet: .. Using the full-length TRIM21 gene as a template, four constructs for the expression of individual domains C-terminally fused to MBP were generated by polymerase chain reaction (PCR) and inserted into the NcoI and BamHI sites of the pET15b vector by In-Fusion cloning (Takara Bio, Kusatsu, Japan) (1): residues 1–85 encompassing the RING domain (2), residues 86–130 (B-Box) (3), residues 131–276 (Coiled–Coil) and (4) residues 277–475 (PRY/SPRY). .. To eliminate the inherent IgG (Fc) binding activity of the PRY/SPRY domain, the critical residues Trp 381 and Trp 383 were mutated to www.scandjrheumatol.se alanine by QuickChange (Agilent, Santa Clara, CA, USA) site-directed mutagenesis reactions.

    Article Title: Cancer-linked ANKRD26-RET uses an unusual combination of pathomechanisms leading to strongly increased cell proliferation.
    Article Snippet: .. Human ANKRD26-GFP was cloned by PCR using EST clone 6830677 (NM_014915.2) as template and subcloned into pEGFP-N3 (Clontech) using KpnI und BamHI as restriction sites, as described before [19]. ..

    Article Title: An ancestral glutamate receptor mediates cell volume regulation during high K + stress in cyanobacteria
    Article Snippet: .. DNA sequences encoding GluR0 and KirBac6.1 were PCR amplified using Synechocystis genomic DNA as a template and cloned into the BamHI/SalI sites of pSTV28 (TaKaRa) to generate pSTV28-GluR0 and pSTV28-KirBac6.1, respectively. ..

    Cloning:

    Article Title: Immunoglobulin G and immunoglobulin A anti-Ro52 in plasma and saliva show little association with disease activity in patients with primary Sjögren's disease.
    Article Snippet: .. Using the full-length TRIM21 gene as a template, four constructs for the expression of individual domains C-terminally fused to MBP were generated by polymerase chain reaction (PCR) and inserted into the NcoI and BamHI sites of the pET15b vector by In-Fusion cloning (Takara Bio, Kusatsu, Japan) (1): residues 1–85 encompassing the RING domain (2), residues 86–130 (B-Box) (3), residues 131–276 (Coiled–Coil) and (4) residues 277–475 (PRY/SPRY). .. To eliminate the inherent IgG (Fc) binding activity of the PRY/SPRY domain, the critical residues Trp 381 and Trp 383 were mutated to www.scandjrheumatol.se alanine by QuickChange (Agilent, Santa Clara, CA, USA) site-directed mutagenesis reactions.

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Article Title: Neural signaling contributes to heart formation and growth in the invertebrate chordate, Ciona robusta
    Article Snippet: VACHT>eCFP cassette [ ] of pSPCiVACHTC [ ] was amplified by PCR using with PrimeSTAR HS DNA polymerase (Takara Bio Japan). .. The fragment was inserted into the BamHI site of pMiLRneo [ ] by In-Fusion HD Cloning kit (Clontech) to create pMiCiVACHTC . pMiCiVACHTC was electroporated with Minos transposase mRNA to create stable transgenic line according to the previous method [ ]. ..

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Ligation:

    Article Title: DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains
    Article Snippet: Nine representative TYLCV sequences originating from Australia, China, Egypt, Japan, Jordan, Portugal, Spain, Sweden, and the United States were synthesized (Macrogen, Korea), and we cloned them into the binary vector pCAMBIA1303 using Kpn I and Hin dIII (Takara, Japan). .. Infectious clones of six Korean TYLCV isolates (collected in 2023) were constructed by amplifying IC1 and IC2 fragments, cloning them into the pJET vector (Thermo Fisher Scientific, USA), and assembling into the pCAMBIA1303 (1.1-mer) via a three-piece ligation using Bam HI, Sph I, and Hin dIII (Takara) ( ). ..

    Transgenic Assay:

    Article Title: Neural signaling contributes to heart formation and growth in the invertebrate chordate, Ciona robusta
    Article Snippet: VACHT>eCFP cassette [ ] of pSPCiVACHTC [ ] was amplified by PCR using with PrimeSTAR HS DNA polymerase (Takara Bio Japan). .. The fragment was inserted into the BamHI site of pMiLRneo [ ] by In-Fusion HD Cloning kit (Clontech) to create pMiCiVACHTC . pMiCiVACHTC was electroporated with Minos transposase mRNA to create stable transgenic line according to the previous method [ ]. ..

    Positive Control:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Over Expression:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Sequencing:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..



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