lambda embl3 sp6 t7 vector (TaKaRa)

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TaKaRa lambda embl3 sp6 t7 vector
Lambda Embl3 Sp6 T7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+embl3+sp6/pmc11115939-207-37-41
Average 86 stars, based on 1 article reviews

lambda embl3 sp6 t7 vector - by Bioz Stars, 2026-10

86/100 stars

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Labeling:

Article Title: S-adenosyl-L-methionine synthetase promoter and its use in expression of transgenic genes in plants
Article Snippet: .. This PstI fragment was labeled with α-32P-dCTP, as described in Example 3, and used as a probe to screen a soybean genomic DNA library that had been constructed in a EMBL3 SP6/T7 vector (ClonTech, Palo Alto, Calif.). .. The library was plated with LE392 (ClonTech) cells at 50,000 plaque forming units (pfu) per 150 mm NZCYM agar plate (GIBCO BRL).

Construct:

Article Title: Method of diagnosis of inflammatory disease using eotaxin antibodies
Article Snippet: .. A human genomic phage library constructed in the EMBL3 SP6/T7 vector, purchased from CLONTECH Laboratories, Inc. (Palo Alto, Calif.), was screened with the 201 bp PCR fragment to obtain a full-length clone. ..

Article Title: Molecular mechanisms of herbicide-inducible gene expression of tobacco CYP71AH11 metabolizing the herbicide chlorotoluron.
Article Snippet: Tobacco cytochrome P450 (CYP) 71AH11 metabolized the herbicide chlorotoluron, and its mRNA level was increased in tobacco culture cells by the treatment of 2,4-D.. In order to clarify molecular mechanisms of induced gene expression of CYP71AH11 by herbicide treatment, a 1574-bp 50-flanking region of CYP71AH11 was cloned, ligated to the reporter b-glucuronidase (GUS) gene, and then transformed into tobacco plants.. The GUS activity in the transgenic tobacco plants was highly induced by bromoxynil treatment, followed by 2,4-D. Chlorotoluron was slightly increased the GUS activity.

Sequencing:

Article Title: Tissue specific promoters
Article Snippet: .. The 5′ and 3′ primer of the “EMBL3 LD-Insert Screening Amplimer Set” (Clontech #9104-1, Heidelberg, Germany), which are phage arm specific comprise the nucleotide sequence CTG CTT CTA ATA GAG TCT TGC TGC AGA CAA ACT GCG CAA C (SEQ ID NO: 5) and TGA ACA CTC GTC CGA GAA TAA CGA GTG GAT CTG GGT C (SEQ ID NO: 6), respectively. .. The amplification of genomic sugar beet DNA fragments was carried out with the help of the “Advantage Genomic PCR Kit” (Clonetech #K1906-1, Heidelberg, Germany).

Polymerase Chain Reaction:

Clone Assay:

Article Title:
Article Snippet: .. Genomic clones of the porcine ML gene were isolated by screening a pig genomic library in EMBL3 (Clontech Inc.) with pR45. ..

Isolation:

Article Title:
Article Snippet: .. Genomic clones of the porcine ML gene were isolated by screening a pig genomic library in EMBL3 (Clontech Inc.) with pR45. ..

Hybridization:

Article Title: Novel glutamate dehydrogenase genes show increased transcript and protein abundances in mature tomato fruits.
Article Snippet: NAD(P)H-glutamate dehydrogenase (GDH, EC 1.4.1.3) contributes to the control of glutamate homeostasis in all living organisms.. In bacteria and animals, GDH is a homohexamer allosterically regulated, whereas in plants NADH-GDH (EC 1.4.1.2) is also found as heterohexamer of and -subunits, but its regulation remains undefined.. In tomato (Solanum lycopersicum), GDH activity increases during the fruit ripening along with the content of free glutamate, the most abundant amino acid of ripe fruit involved in conferring the genuine tomato flavour.

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