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lambda phage vector embl3 sp6 7  (TaKaRa)


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    Structured Review

    TaKaRa lambda phage vector embl3 sp6 7
    Lambda Phage Vector Embl3 Sp6 7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+embl3+sp6/BamH+I/pm10645719-62-20-27
    Average 99 stars, based on 14737 article reviews
    lambda phage vector embl3 sp6 7 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET.
    Article Snippet: .. The PCR fragment was cloned between XhoI and BamHI restriction sites of a mCherry-N1 (Clontech) vector. ..

    Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress
    Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat# 638948). .. pENTRPrimPol-myc-FLAG was recombined into CSIV-TRE-RfA-UbC-Puro (a kind gift from Dr. Nakanishi) using the GateWay system (Invitrogen, Cat# 11791-020).

    Article Title: Aberrant splicing caused by a homozygous AK2 c.498 + 1G > A variant in a patient with reticular dysgenesis and concurrent B-cell acute lymphoblastic leukemia.
    Article Snippet: Reticular dysgenesis (RD) is a rare autosomal recessive severe combined immunodeficiency characterized by profound neutropenia, T-cell lymphopenia and sensorineural hearing loss, and is caused by biallelic loss-of-function variants in AK2.. We identified a homozygous AK2 splice-site variant (c.498+1G>A) in a boy with RD and concurrent B-cell acute lymphoblastic leukemia (B-ALL), a clinical combination not previously reported in association with an AK2 variant.. An in vitro minigene assay showed that c.498+1G>A disrupts the canonical 5′ splice donor site and generates an aberrant transcript carrying a 4-bp intronic insertion after exon 5, which causes a frameshift and introduces a premature termination codon; the transcript is predicted to be degraded by nonsense-mediated mRNA decay or, if translated, to yield a non-functional truncated protein, leading to loss of AK2 function.

    Article Title: Macroscopic and microscopic visualization of Pseudomonas syringae pv. tabaci infection using complementary bioluminescent and fluorescent reporter systems
    Article Snippet: The nptII promoter was also amplified by PCR from pDSK, fused to sgfp amplified from pGWB5, and inserted into the BamHI site of pK18IP using NEBuilder. .. The gltA promoter was amplified by PCR from the Pta genome, fused to sgfp amplified from pGWB5 (Nakagawa et al. 2007), and inserted into the BamHI site of pK18IP using In-Fusion Snap Assembly Master Mix (Takara, Shiga, Japan). ..

    Article Title: Development of a genetically engineered multivalent subunit vaccine against "struck", "braxy", lamb dysentery, and enterotoxemia in sheep.
    Article Snippet: BL21 (DE3) competent cells were obtained from Beijing TransGen Biotech Co., Ltd. (Beijing, China). .. Restriction enzymes BamHI and XhoI, T4 DNA ligase, a 2000 DNA marker, sodium dodecyl sulfate, isopropyl-β-D-thiogalactopyranoside (IPTG), and Taq PCR Master Mix were provided by Takara Biotechnology Co., Ltd. (Dalian, China). .. Agarose, a DNA extraction kit, a DNA rapid purification and recovery kit, a plasmid rapid isolation kit, and a pre-stained protein marker were obtained from Beijing TransGen Biotech Co., Ltd. Ni-NTA spin columns (5 mL) and sizeexclusion chromatography columns (Superdex 2000) were obtained from GE Healthcare Bio-Sciences (Pittsburgh, PA, USA).

    Clone Assay:

    Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET.
    Article Snippet: .. The PCR fragment was cloned between XhoI and BamHI restriction sites of a mCherry-N1 (Clontech) vector. ..

    Article Title: Aberrant splicing caused by a homozygous AK2 c.498 + 1G > A variant in a patient with reticular dysgenesis and concurrent B-cell acute lymphoblastic leukemia.
    Article Snippet: Reticular dysgenesis (RD) is a rare autosomal recessive severe combined immunodeficiency characterized by profound neutropenia, T-cell lymphopenia and sensorineural hearing loss, and is caused by biallelic loss-of-function variants in AK2.. We identified a homozygous AK2 splice-site variant (c.498+1G>A) in a boy with RD and concurrent B-cell acute lymphoblastic leukemia (B-ALL), a clinical combination not previously reported in association with an AK2 variant.. An in vitro minigene assay showed that c.498+1G>A disrupts the canonical 5′ splice donor site and generates an aberrant transcript carrying a 4-bp intronic insertion after exon 5, which causes a frameshift and introduces a premature termination codon; the transcript is predicted to be degraded by nonsense-mediated mRNA decay or, if translated, to yield a non-functional truncated protein, leading to loss of AK2 function.

    Plasmid Preparation:

    Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET.
    Article Snippet: .. The PCR fragment was cloned between XhoI and BamHI restriction sites of a mCherry-N1 (Clontech) vector. ..

    Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress
    Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat# 638948). .. pENTRPrimPol-myc-FLAG was recombined into CSIV-TRE-RfA-UbC-Puro (a kind gift from Dr. Nakanishi) using the GateWay system (Invitrogen, Cat# 11791-020).

    Article Title: PI3K is the dominant signalling pathway recruited by IRS proteins to mediate their effects on metabolism and growth.
    Article Snippet: .. Mutant cDNAs were then subcloned 19 into the BamHI/NotI sites of the expression vector pIRES2-EGFP (Clontech). ..

    Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation
    Article Snippet: .. The purified luxS-eGFP fusion product was double-digested with EcoRI and BamHI (Takara Bio Inc.) at 37 ◦C for 3 h, and the pUC-19 vector was similarly digested at the corresponding sites. .. The digested fragments were ligated into pUC-19 using T4 DNA ligase (Takara Bio Inc., Cat. No. 2011B) following the manufacturer’s protocol.

    Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells.
    Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the B16-F1 cDNA library and inserted into the BamHI and XhoI sites of plasmid pGADT7 (Clontech), and the amplified GBD/FH3 region of murine Daam2 (Uniprot ID:Q80U19, residues 40–420) was inserted into the XmaI and XhoI sites of pGADT7 to generate the bait plasmids. .. For expression of constitutively-active and dominantnegative GTPases in E. coli, the coding sequences were amplified from the constructs mentioned above and inserted either into the BamHI and SalI sites (RhoA, RhoC, RhoD, Rac1, and Cdc42) or into the SalI and NotI sites (RhoB and Rif ) of plasmid pGEX6-P-3 (GE Healthcare).

    Amplification:

    Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress
    Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat# 638948). .. pENTRPrimPol-myc-FLAG was recombined into CSIV-TRE-RfA-UbC-Puro (a kind gift from Dr. Nakanishi) using the GateWay system (Invitrogen, Cat# 11791-020).

    Article Title: Macroscopic and microscopic visualization of Pseudomonas syringae pv. tabaci infection using complementary bioluminescent and fluorescent reporter systems
    Article Snippet: The nptII promoter was also amplified by PCR from pDSK, fused to sgfp amplified from pGWB5, and inserted into the BamHI site of pK18IP using NEBuilder. .. The gltA promoter was amplified by PCR from the Pta genome, fused to sgfp amplified from pGWB5 (Nakagawa et al. 2007), and inserted into the BamHI site of pK18IP using In-Fusion Snap Assembly Master Mix (Takara, Shiga, Japan). ..

    Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells.
    Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the B16-F1 cDNA library and inserted into the BamHI and XhoI sites of plasmid pGADT7 (Clontech), and the amplified GBD/FH3 region of murine Daam2 (Uniprot ID:Q80U19, residues 40–420) was inserted into the XmaI and XhoI sites of pGADT7 to generate the bait plasmids. .. For expression of constitutively-active and dominantnegative GTPases in E. coli, the coding sequences were amplified from the constructs mentioned above and inserted either into the BamHI and SalI sites (RhoA, RhoC, RhoD, Rac1, and Cdc42) or into the SalI and NotI sites (RhoB and Rif ) of plasmid pGEX6-P-3 (GE Healthcare).

    Cloning:

    Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress
    Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat# 638948). .. pENTRPrimPol-myc-FLAG was recombined into CSIV-TRE-RfA-UbC-Puro (a kind gift from Dr. Nakanishi) using the GateWay system (Invitrogen, Cat# 11791-020).

    Article Title: Aberrant splicing caused by a homozygous AK2 c.498 + 1G > A variant in a patient with reticular dysgenesis and concurrent B-cell acute lymphoblastic leukemia.
    Article Snippet: Reticular dysgenesis (RD) is a rare autosomal recessive severe combined immunodeficiency characterized by profound neutropenia, T-cell lymphopenia and sensorineural hearing loss, and is caused by biallelic loss-of-function variants in AK2.. We identified a homozygous AK2 splice-site variant (c.498+1G>A) in a boy with RD and concurrent B-cell acute lymphoblastic leukemia (B-ALL), a clinical combination not previously reported in association with an AK2 variant.. An in vitro minigene assay showed that c.498+1G>A disrupts the canonical 5′ splice donor site and generates an aberrant transcript carrying a 4-bp intronic insertion after exon 5, which causes a frameshift and introduces a premature termination codon; the transcript is predicted to be degraded by nonsense-mediated mRNA decay or, if translated, to yield a non-functional truncated protein, leading to loss of AK2 function.

    Mutagenesis:

    Article Title: PI3K is the dominant signalling pathway recruited by IRS proteins to mediate their effects on metabolism and growth.
    Article Snippet: .. Mutant cDNAs were then subcloned 19 into the BamHI/NotI sites of the expression vector pIRES2-EGFP (Clontech). ..

    Expressing:

    Article Title: PI3K is the dominant signalling pathway recruited by IRS proteins to mediate their effects on metabolism and growth.
    Article Snippet: .. Mutant cDNAs were then subcloned 19 into the BamHI/NotI sites of the expression vector pIRES2-EGFP (Clontech). ..

    Purification:

    Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation
    Article Snippet: .. The purified luxS-eGFP fusion product was double-digested with EcoRI and BamHI (Takara Bio Inc.) at 37 ◦C for 3 h, and the pUC-19 vector was similarly digested at the corresponding sites. .. The digested fragments were ligated into pUC-19 using T4 DNA ligase (Takara Bio Inc., Cat. No. 2011B) following the manufacturer’s protocol.

    Marker:

    Article Title: Development of a genetically engineered multivalent subunit vaccine against "struck", "braxy", lamb dysentery, and enterotoxemia in sheep.
    Article Snippet: BL21 (DE3) competent cells were obtained from Beijing TransGen Biotech Co., Ltd. (Beijing, China). .. Restriction enzymes BamHI and XhoI, T4 DNA ligase, a 2000 DNA marker, sodium dodecyl sulfate, isopropyl-β-D-thiogalactopyranoside (IPTG), and Taq PCR Master Mix were provided by Takara Biotechnology Co., Ltd. (Dalian, China). .. Agarose, a DNA extraction kit, a DNA rapid purification and recovery kit, a plasmid rapid isolation kit, and a pre-stained protein marker were obtained from Beijing TransGen Biotech Co., Ltd. Ni-NTA spin columns (5 mL) and sizeexclusion chromatography columns (Superdex 2000) were obtained from GE Healthcare Bio-Sciences (Pittsburgh, PA, USA).

    cDNA Library Assay:

    Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells.
    Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the B16-F1 cDNA library and inserted into the BamHI and XhoI sites of plasmid pGADT7 (Clontech), and the amplified GBD/FH3 region of murine Daam2 (Uniprot ID:Q80U19, residues 40–420) was inserted into the XmaI and XhoI sites of pGADT7 to generate the bait plasmids. .. For expression of constitutively-active and dominantnegative GTPases in E. coli, the coding sequences were amplified from the constructs mentioned above and inserted either into the BamHI and SalI sites (RhoA, RhoC, RhoD, Rac1, and Cdc42) or into the SalI and NotI sites (RhoB and Rif ) of plasmid pGEX6-P-3 (GE Healthcare).



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