lambda phage vector embl3 sp6 7 (TaKaRa)
99
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TaKaRa
lambda phage vector embl3 sp6 7
Lambda Phage Vector Embl3 Sp6 7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+embl3+sp6/BamH+I/pm10645719-62-20-27
Average 99 stars, based on 14737 article reviews
Lambda Phage Vector Embl3 Sp6 7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+embl3+sp6/BamH+I/pm10645719-62-20-27
Average 99 stars, based on 14737 article reviews
lambda phage vector embl3 sp6 7 - by Bioz Stars,
2026-10
99/100 stars
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Polymerase Chain Reaction:Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET. Article Snippet: .. The PCR fragment was cloned between XhoI and Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the Article Title: Aberrant splicing caused by a homozygous AK2 c.498 + 1G > A variant in a patient with reticular dysgenesis and concurrent B-cell acute lymphoblastic leukemia. Article Snippet: Reticular dysgenesis (RD) is a rare autosomal recessive severe combined immunodeficiency characterized by profound neutropenia, T-cell lymphopenia and sensorineural hearing loss, and is caused by biallelic loss-of-function variants in AK2.. We identified a homozygous AK2 splice-site variant (c.498+1G>A) in a boy with RD and concurrent B-cell acute lymphoblastic leukemia (B-ALL), a clinical combination not previously reported in association with an AK2 variant.. An in vitro minigene assay showed that c.498+1G>A disrupts the canonical 5′ splice donor site and generates an aberrant transcript carrying a 4-bp intronic insertion after exon 5, which causes a frameshift and introduces a premature termination codon; the transcript is predicted to be degraded by nonsense-mediated mRNA decay or, if translated, to yield a non-functional truncated protein, leading to loss of AK2 function. Article Title: Macroscopic and microscopic visualization of Pseudomonas syringae pv. tabaci infection using complementary bioluminescent and fluorescent reporter systems Article Snippet: The nptII promoter was also amplified by PCR from pDSK, fused to sgfp amplified from pGWB5, and inserted into the BamHI site of pK18IP using NEBuilder. .. The gltA promoter was amplified by PCR from the Pta genome, fused to sgfp amplified from pGWB5 (Nakagawa et al. 2007), and inserted into the Article Title: Development of a genetically engineered multivalent subunit vaccine against "struck", "braxy", lamb dysentery, and enterotoxemia in sheep. Article Snippet: BL21 (DE3) competent cells were obtained from Beijing TransGen Biotech Co., Ltd. (Beijing, China). .. Clone Assay:Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET. Article Snippet: .. The PCR fragment was cloned between XhoI and Article Title: Aberrant splicing caused by a homozygous AK2 c.498 + 1G > A variant in a patient with reticular dysgenesis and concurrent B-cell acute lymphoblastic leukemia. Article Snippet: Reticular dysgenesis (RD) is a rare autosomal recessive severe combined immunodeficiency characterized by profound neutropenia, T-cell lymphopenia and sensorineural hearing loss, and is caused by biallelic loss-of-function variants in AK2.. We identified a homozygous AK2 splice-site variant (c.498+1G>A) in a boy with RD and concurrent B-cell acute lymphoblastic leukemia (B-ALL), a clinical combination not previously reported in association with an AK2 variant.. An in vitro minigene assay showed that c.498+1G>A disrupts the canonical 5′ splice donor site and generates an aberrant transcript carrying a 4-bp intronic insertion after exon 5, which causes a frameshift and introduces a premature termination codon; the transcript is predicted to be degraded by nonsense-mediated mRNA decay or, if translated, to yield a non-functional truncated protein, leading to loss of AK2 function. Plasmid Preparation:Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET. Article Snippet: .. The PCR fragment was cloned between XhoI and Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the Article Title: PI3K is the dominant signalling pathway recruited by IRS proteins to mediate their effects on metabolism and growth. Article Snippet: .. Mutant cDNAs were then subcloned 19 into the Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation Article Snippet: .. The purified luxS-eGFP fusion product was double-digested with EcoRI and Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the Amplification:Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the Article Title: Macroscopic and microscopic visualization of Pseudomonas syringae pv. tabaci infection using complementary bioluminescent and fluorescent reporter systems Article Snippet: The nptII promoter was also amplified by PCR from pDSK, fused to sgfp amplified from pGWB5, and inserted into the BamHI site of pK18IP using NEBuilder. .. The gltA promoter was amplified by PCR from the Pta genome, fused to sgfp amplified from pGWB5 (Nakagawa et al. 2007), and inserted into the Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the Cloning:Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the Article Title: Aberrant splicing caused by a homozygous AK2 c.498 + 1G > A variant in a patient with reticular dysgenesis and concurrent B-cell acute lymphoblastic leukemia. Article Snippet: Reticular dysgenesis (RD) is a rare autosomal recessive severe combined immunodeficiency characterized by profound neutropenia, T-cell lymphopenia and sensorineural hearing loss, and is caused by biallelic loss-of-function variants in AK2.. We identified a homozygous AK2 splice-site variant (c.498+1G>A) in a boy with RD and concurrent B-cell acute lymphoblastic leukemia (B-ALL), a clinical combination not previously reported in association with an AK2 variant.. An in vitro minigene assay showed that c.498+1G>A disrupts the canonical 5′ splice donor site and generates an aberrant transcript carrying a 4-bp intronic insertion after exon 5, which causes a frameshift and introduces a premature termination codon; the transcript is predicted to be degraded by nonsense-mediated mRNA decay or, if translated, to yield a non-functional truncated protein, leading to loss of AK2 function. Mutagenesis:Article Title: PI3K is the dominant signalling pathway recruited by IRS proteins to mediate their effects on metabolism and growth. Article Snippet: .. Mutant cDNAs were then subcloned 19 into the Expressing:Article Title: PI3K is the dominant signalling pathway recruited by IRS proteins to mediate their effects on metabolism and growth. Article Snippet: .. Mutant cDNAs were then subcloned 19 into the Purification:Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation Article Snippet: .. The purified luxS-eGFP fusion product was double-digested with EcoRI and Marker:Article Title: Development of a genetically engineered multivalent subunit vaccine against "struck", "braxy", lamb dysentery, and enterotoxemia in sheep. Article Snippet: BL21 (DE3) competent cells were obtained from Beijing TransGen Biotech Co., Ltd. (Beijing, China). .. cDNA Library Assay:Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the |