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lambda dash ii vector  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda dash ii vector
    Lambda Dash Ii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+dash%C2%AEii/pmc04172706-59-26-30
    Average 90 stars, based on 1 article reviews
    lambda dash ii vector - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains
    Article Snippet: .. Genomic fragments were ligated into the Lambda Dash II vector (Stratagene) predigested with BamHI and packaged using the Gigapack III XL packaging extract (Stratagene). ..

    Article Title: Unusually short tandem repeats appear to reach chromosome ends of Rhynchosciara americana (Diptera: Sciaridae).
    Article Snippet: The characterisation of sequences at chromosome ends of Rhynchosciara americana was continued with the screening of a genomic library using as a probe a short repeat identified in a previous report (M-22, 22 bp) which was found to be specific for noncentromeric termini of this species.. Simple repeats, complex tandem and apparently dispersed repeats were present in the genomic clones analysed.. Repetitive sequences do not define individual chromosome tips as they were found in all noncentromeric ends.

    Article Title: Curiously composite structures of a retrotransposon and a complex repeat associated with chromosome ends of Rhynchosciara americana (Diptera: Sciaridae).
    Article Snippet: In Drosophila, telomere retrotransposons counterbalance the loss of telomeric DNA.. The exceptional mechanism of telomere recovery characterized in Drosophila has not been found in lower dipterans (Nematocera).. However, a retroelement resembling a telomere transposon and termed “RaTART” has been described in the nematoceran Rhynchosciara americana.

    Article Title: Structure and Polymorphism of the Major Histocompatibility Complex Class II Region in the Japanese Crested Ibis, Nipponia nippon
    Article Snippet: .. Digested fragments (15–23 kb) were gel purified using the Wizard SV Gel and PCR Clean-Up system (Promega), according to the manufacturer’s instructions, and ligated into the Lambda DASH II vector (Stratagene). .. The ligated DNA mixture was then packaged using Gigapack III Gold or XL packaging extract (Stratagene).

    Article Title: Mitochondrial DNA Phylogeny of Cultivated and Wild Beets: Relationships Among Cytoplasmic Male-Sterility-Inducing and Nonsterilizing Cytoplasms
    Article Snippet: .. DNA fragments of ∼18 kbp were fractionated by centrifugation and cloned into the Lambda DASH II vector (Stratagene) according to standard procedures. ..

    Article Title: Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
    Article Snippet: .. The amplified PCR fragment (approximately 1100 bp) was labeled with [32P]-dCTP using a Random Primer DNA Labeling Kit (Takara Bio, Otsu, Japan), and used as a probe to screen positive clones from a F. velutipes genomic library (comprising w20,000 clones) constructed in the lambda Dash II vector (Agilent Technologies, Santa Clara, CA, USA) (4). .. The full-length Fv-ada cDNA was amplified from total RNA prepared from cells at primordial stage (3) using an RNA PCR kit (Takara Bio).

    Cloning:

    Article Title: Unusually short tandem repeats appear to reach chromosome ends of Rhynchosciara americana (Diptera: Sciaridae).
    Article Snippet: The characterisation of sequences at chromosome ends of Rhynchosciara americana was continued with the screening of a genomic library using as a probe a short repeat identified in a previous report (M-22, 22 bp) which was found to be specific for noncentromeric termini of this species.. Simple repeats, complex tandem and apparently dispersed repeats were present in the genomic clones analysed.. Repetitive sequences do not define individual chromosome tips as they were found in all noncentromeric ends.

    Article Title: Curiously composite structures of a retrotransposon and a complex repeat associated with chromosome ends of Rhynchosciara americana (Diptera: Sciaridae).
    Article Snippet: In Drosophila, telomere retrotransposons counterbalance the loss of telomeric DNA.. The exceptional mechanism of telomere recovery characterized in Drosophila has not been found in lower dipterans (Nematocera).. However, a retroelement resembling a telomere transposon and termed “RaTART” has been described in the nematoceran Rhynchosciara americana.

    Purification:

    Article Title: Structure and Polymorphism of the Major Histocompatibility Complex Class II Region in the Japanese Crested Ibis, Nipponia nippon
    Article Snippet: .. Digested fragments (15–23 kb) were gel purified using the Wizard SV Gel and PCR Clean-Up system (Promega), according to the manufacturer’s instructions, and ligated into the Lambda DASH II vector (Stratagene). .. The ligated DNA mixture was then packaged using Gigapack III Gold or XL packaging extract (Stratagene).

    Polymerase Chain Reaction:

    Article Title: Structure and Polymorphism of the Major Histocompatibility Complex Class II Region in the Japanese Crested Ibis, Nipponia nippon
    Article Snippet: .. Digested fragments (15–23 kb) were gel purified using the Wizard SV Gel and PCR Clean-Up system (Promega), according to the manufacturer’s instructions, and ligated into the Lambda DASH II vector (Stratagene). .. The ligated DNA mixture was then packaged using Gigapack III Gold or XL packaging extract (Stratagene).

    Article Title: Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
    Article Snippet: .. The amplified PCR fragment (approximately 1100 bp) was labeled with [32P]-dCTP using a Random Primer DNA Labeling Kit (Takara Bio, Otsu, Japan), and used as a probe to screen positive clones from a F. velutipes genomic library (comprising w20,000 clones) constructed in the lambda Dash II vector (Agilent Technologies, Santa Clara, CA, USA) (4). .. The full-length Fv-ada cDNA was amplified from total RNA prepared from cells at primordial stage (3) using an RNA PCR kit (Takara Bio).

    Centrifugation:

    Article Title: Mitochondrial DNA Phylogeny of Cultivated and Wild Beets: Relationships Among Cytoplasmic Male-Sterility-Inducing and Nonsterilizing Cytoplasms
    Article Snippet: .. DNA fragments of ∼18 kbp were fractionated by centrifugation and cloned into the Lambda DASH II vector (Stratagene) according to standard procedures. ..

    Clone Assay:

    Article Title: Mitochondrial DNA Phylogeny of Cultivated and Wild Beets: Relationships Among Cytoplasmic Male-Sterility-Inducing and Nonsterilizing Cytoplasms
    Article Snippet: .. DNA fragments of ∼18 kbp were fractionated by centrifugation and cloned into the Lambda DASH II vector (Stratagene) according to standard procedures. ..

    Article Title: Cross-talk between Aryl Hydrocarbon Receptor and Mitogen-Activated Protein Kinase Signaling Pathway in Liver Cancer through c-raf Transcriptional Regulation
    Article Snippet: .. A rat genomic library in the Lambda DASH II vector (obtained from Stratagene) was plated at 25,000 plaqueforming units per 15-cm plate on SRB as host strain and screened for c-raf genomic clones with 32P-labeled, 260-bp EcoRI-Msp I 5¶-upstream fragment of the rat cDNA subclone, as described above. ..

    Article Title: Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
    Article Snippet: .. The amplified PCR fragment (approximately 1100 bp) was labeled with [32P]-dCTP using a Random Primer DNA Labeling Kit (Takara Bio, Otsu, Japan), and used as a probe to screen positive clones from a F. velutipes genomic library (comprising w20,000 clones) constructed in the lambda Dash II vector (Agilent Technologies, Santa Clara, CA, USA) (4). .. The full-length Fv-ada cDNA was amplified from total RNA prepared from cells at primordial stage (3) using an RNA PCR kit (Takara Bio).

    Sulforhodamine B Assay:

    Article Title: Cross-talk between Aryl Hydrocarbon Receptor and Mitogen-Activated Protein Kinase Signaling Pathway in Liver Cancer through c-raf Transcriptional Regulation
    Article Snippet: .. A rat genomic library in the Lambda DASH II vector (obtained from Stratagene) was plated at 25,000 plaqueforming units per 15-cm plate on SRB as host strain and screened for c-raf genomic clones with 32P-labeled, 260-bp EcoRI-Msp I 5¶-upstream fragment of the rat cDNA subclone, as described above. ..

    Amplification:

    Article Title: Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
    Article Snippet: .. The amplified PCR fragment (approximately 1100 bp) was labeled with [32P]-dCTP using a Random Primer DNA Labeling Kit (Takara Bio, Otsu, Japan), and used as a probe to screen positive clones from a F. velutipes genomic library (comprising w20,000 clones) constructed in the lambda Dash II vector (Agilent Technologies, Santa Clara, CA, USA) (4). .. The full-length Fv-ada cDNA was amplified from total RNA prepared from cells at primordial stage (3) using an RNA PCR kit (Takara Bio).

    Labeling:

    Article Title: Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
    Article Snippet: .. The amplified PCR fragment (approximately 1100 bp) was labeled with [32P]-dCTP using a Random Primer DNA Labeling Kit (Takara Bio, Otsu, Japan), and used as a probe to screen positive clones from a F. velutipes genomic library (comprising w20,000 clones) constructed in the lambda Dash II vector (Agilent Technologies, Santa Clara, CA, USA) (4). .. The full-length Fv-ada cDNA was amplified from total RNA prepared from cells at primordial stage (3) using an RNA PCR kit (Takara Bio).

    Random Primer DNA Labeling:

    Article Title: Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
    Article Snippet: .. The amplified PCR fragment (approximately 1100 bp) was labeled with [32P]-dCTP using a Random Primer DNA Labeling Kit (Takara Bio, Otsu, Japan), and used as a probe to screen positive clones from a F. velutipes genomic library (comprising w20,000 clones) constructed in the lambda Dash II vector (Agilent Technologies, Santa Clara, CA, USA) (4). .. The full-length Fv-ada cDNA was amplified from total RNA prepared from cells at primordial stage (3) using an RNA PCR kit (Takara Bio).

    Construct:

    Article Title: Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
    Article Snippet: .. The amplified PCR fragment (approximately 1100 bp) was labeled with [32P]-dCTP using a Random Primer DNA Labeling Kit (Takara Bio, Otsu, Japan), and used as a probe to screen positive clones from a F. velutipes genomic library (comprising w20,000 clones) constructed in the lambda Dash II vector (Agilent Technologies, Santa Clara, CA, USA) (4). .. The full-length Fv-ada cDNA was amplified from total RNA prepared from cells at primordial stage (3) using an RNA PCR kit (Takara Bio).



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