laemlli loading buffer (Millipore)

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Millipore laemlli loading buffer
Laemlli Loading Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/laemlli+loading+buffer/laemmli+loading+buffer/pm30078603-88-5-8
Average 90 stars, based on 1 article reviews

laemlli loading buffer - by Bioz Stars, 2026-10

90/100 stars

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SDS Page:

Article Title: A protease-activatable luminescent biosensor and reporter cell line for authentic SARS-CoV-2 infection
Article Snippet: Washed cell pellets were lysed in PBS + 1% Triton supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific) for 30 min on wet ice. .. Post-nuclear supernatants were heated in Laemelli Loading Buffer for 5 min at 95°C, separated by SDS-PAGE and transferred to Immobilon-P membrane (Millipore). .. Membranes were blocked in PBS/5% non-fat dried milk (Marvel)/0.2% Tween and probed with the indicated primary antibody overnight at 4 C. Reactive bands were visualised using HRP-conjugated secondary antibodies and SuperSignal West Pico or Dura chemiluminescent substrates (Thermo Scientific).

Membrane:

Article Title: Histone Acetylation Defects in Brain Precursor Cells: A Potential Pathogenic Mechanism Causing Proliferation and Differentiation Dysfunctions in Mitochondrial Aspartate-Glutamate Carrier Isoform 1 Deficiency
Article Snippet: .. Each sample (20 μg) was resolved in SDS–PAGE with Laemli loading buffer (Sigma-Aldrich, St Louis, MO, United States) and transferred onto a nitrocellulose membrane (GE Healthcare Life Sciences, Little Chalfont, United Kingdom) for the reaction with the following primary antibodies: Anti-acetyl-Histone H3 (Millipore, Burlington, Massachusetts, United States; Cat# 06-599, RRID:AB_2115283 ), CBP (D6C5) (Cell Signaling Technology, Danvers, Massachusetts; Cat# 7389, RRID:AB_2616020 ), CNPase (Cell Signaling; Cat# 5664, RRID:AB_10705455 ), CREB (48H2) (Cell Signaling; Cat# 9197, RRID:AB_331277 ), c-Myc (N-262) (SantaCruz Biotechnology, Dallas, Texas, United States; Cat# sc-764, RRID:AB_631276 ), Anti-Doublecortin (Abcam; Cat# ab18723, RRID:AB_732011 ), GAPDH (SantaCruz Biotechnology, Dallas, Texas, United States; Cat# sc-32233, RRID:AB_627679 ), GFAP (Dakopatts (Agilent Technologies), Santa Clara, California, United States; Cat# sc-33673, RRID:AB_627673 ), Histone Deacetylase 1 (HDAC1) (Cell Signaling; Cat# 2062, RRID:AB_2118523 ), HDAC2 (D6S5P) (Cell Signaling; Cat# 2540, RRID:AB_2116822 ), Histone Deacetylase 3 (HDAC3) (Cell Signaling; Cat# 2632, RRID:AB_331545 ), Histone Deacetylase 4 (HDAC4) (Cell Signaling; Cat# 2072, RRID:AB_2232915 ), Histone H3 (SantaCruz Biotechnology, Dallas, Texas, United States,; Cat# sc-10809, RRID:AB_2115276 ), HSP60 (Bioss, Woburn, Massachusetts, United States; Cat# bs-0191R-HRP, RRID:AB_11117391 ), MAX (H-2) (SantaCruz Biotechnology, Dallas, Texas, United States; Cat# sc-8011, RRID:AB_627913 ), Anti-NG2 (Abcam, Cambridge, United Kingdom; Cat# ab83178, RRID:AB_10672215 ), NRSF (P-18) (SantaCruz Biotechnology, Dallas, Texas, United States; Cat# sc-15120, RRID:AB_2179628 ), Olig2 (SantaCruz Biotechnology, Dallas, Texas, United States; Cat# sc-48817, RRID:AB_2157550 ), PanMetH3 (MBL International, Woburn, Massachusetts, United States; Cat# LS-A4069, RRID:AB_591306 ), PDGFRα (SantaCruz Biotechnology, Dallas, Texas, United States; Cat# sc-338, RRID:AB_631064 ), Phospho-CREB (Ser133) (Cell Signaling; Cat# 9198, RRID:AB_2561044 ). .. The following specific HRP-linked secondary antibodies (horseradish peroxidase conjugated) were used: Goat anti-Mouse (Jackson ImmunoResearch, West Grove, Pennsylvania, United States; Cat# 115-035-146, RRID:AB_2307392 ), Goat anti-Rabbit (Jackson ImmunoResearch; Cat# 111-035-144, RRID:AB_230739 ), and Mouse anti-Goat (SantaCruz Biotechnology, Dallas, Texas, United States; Cat# sc-2354, RRID:AB_628490 ).

Histone Deacetylase Assay:

Incubation:

Article Title: Norrin mediates tumor-promoting and -suppressive effects in glioblastoma via Notch and Wnt
Article Snippet: Protein concentrations were measured using a benchtop spectrophotometer (Eppendorf). .. After addition of Laemmli loading buffer (Sigma-Aldrich), lysates were incubated at 95°C to ensure complete protein denaturation. ..

Article Title: 2-Methoxyestradiol Damages DNA in Glioblastoma Cells by Regulating nNOS and Heat Shock Proteins
Article Snippet: The Bradford reagent (Sigma-Aldrich, Poland) was used to calculate the protein concentration. .. After that, samples containing 20 μg of protein were combined with Laemmli loading buffer (Sigma-Aldrich, Poland) and incubated at 95 °C for 5 min. Electrophoresis was used to separate the proteins on 4–20% Mini-PROTEAN ® TGX Stain-Free TM Protein Gels (4568093, Bio-rad, Hercules, CA, USA) (120 mV, 90 min). .. Using the Trans-Blot Turbo System, the separated proteins were transferred to Trans-Blot Turbo Midi 0.2 μm PVDF (1704157, Bio-rad, USA).

Western Blot:

Article Title: Neural stem cell self-renewal stimulation by store-operated calcium entries in adult mouse area postrema: influence of leptin
Article Snippet: .. For western blotting analysis, area postrema neurospheres were lysed in Laemmli loading buffer (Sigma Aldrich) and heat denatured at 95°C for 5 min, in parallel with subventricular zone neurospheres used as positive controls ( ). .. All samples were resolved in a 9% sodium dodecyl sulfate-polyacrylamide gel along with pre-stained standard molecular weight markers (BioRad Laboratories, Hercules, CA, USA).

Article Title: Store-Operated Calcium Channels Control Proliferation and Self-Renewal of Cancer Stem Cells from Glioblastoma
Article Snippet: .. For Western blotting analysis, 10 6 cells were lysed in Laemmli loading buffer (Sigma Aldrich). ..

Electrophoresis:

Staining:

Sample Prep:

Article Title: Tumor Necrosis Factor-Like Weak Inducer of Apoptosis Accelerates the Progression of Renal Fibrosis in Lupus Nephritis by Activating SMAD and p38 MAPK in TGF- β 1 Signaling Pathway
Article Snippet: Protein concentrations were determined with the BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). .. From each sample preparation, 60 μ g of total proteins was mixed in Laemmli loading buffer, boiled for 5 min, separated by 10% SDS-PAGE, and then transferred to PVDF blotting membranes (Millipore, MA, USA). .. Membranes were blocked with Tris-buffered saline-Tween/1% nonfat dry milk and incubated overnight at 4°C with goat antibodies specific for TWEAK (1 : 500, Santa Cruz, CA, USA), rabbit antibodies specific for p-p38 MAPK and p38 MAPK (1 : 500; Santa Cruz, CA, USA), goat antibodies specific for TGF- β 1 (1 : 250; Santa Cruz, CA, USA), mouse antibodies specific for COL-1, α -SMA, and E-cadherin (1 : 500; Abcam, Cambridge, UK), rabbit antibodies specific for p-Smad2 and Smad2 (1 : 500; Abcam, Cambridge, UK), and rabbit polyclonal antibody to β -actin (1 : 1000; Sigma Aldrich, Saint Louis, USA).

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