1 ig (Proteintech)

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Proteintech 1 ig
1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/keap1/KEAP1+Antibody/pmc12969009-19-7-4
Average 96 stars, based on 1342 article reviews

1 ig - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: IL-3 Modulates Microglia Polarization and Attenuates Neuroinflammation in Traumatic Brain Injury.
Article Snippet: Extracted proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to PVDF membranes (Millipore, Carrigtwohill, Ireland). .. Membranes were closed using non-degreased milk and then incubated overnight at 4◦C with a series of primary antibodies, including antirabbit iNOS (18985-1-AP, Proteintech, 1:1000), anti-rabbit-NRF2 (ab62352, Abcam, 1:1000), anti-rabbit phosphorylated P65 NF- κB (3033s, CST, 1:1000), anti-rabbit p65 NF- κB (8242s, CST, 1:1000), anti-rabbit I κB- α (4812s, CST, 1:1000), anti-rabbit Vinculin (26520- 1-AP, Proteintech, 1:10000), anti-rabbit LaminB1 (12987-1-AP, Proteintech, 1:10000), anti-rabbit PRDX1 (15816-1-AP, Proteintech,1: 10000), anti-mouse β-actin (TA-09, ZSGB-BIO, 1:5000), anti-rabbit KEAP1 (10503-2-AP, Proteintech 1:2000), anti-rabbit HO-1 (10701-1-AP, Proteintech, 1:3000), horseradish enzymelabelled goat anti-rabbit IgG (ZB-2301, ZSGB-BIO, 1:5000), and horseradish enzyme-labelled goat anti-mouse IgG (ZB-2305, ZSGB-BIO, 1:5000). ..

Article Title: Study on the potential mechanism of Artemisia integrifolia Linn. in diabetic nephropathy: multiscale analysis of network pharmacology and metabolomics.
Article Snippet: Ethnopharmacological relevance: Artemisia integrifolia Linn. (Chinese name: Liuhao, LH) is a traditional Chinese medicine commonly employed for managing diabetes.. However, its pharmacological effects on diabetic nephropathy (DN) remain unclear.. Aim of the study: This study aimed to investigate the effects and underlying mechanisms of LH on DN.

Article Title: Ginsenoside Rh4 Triggers Ferroptosis in Lung Cancer: Targeting KEAP1 / NRF2 / HO-1 and Remodeling Gut Microbiota for Butyrate-Mediated ATF3 Activation.
Article Snippet: Protein samples were resolved on 10% SDS– polyacrylamide gels (UEIandy, Suzhou, China), transferred to PVDF membrane (Keygen BioTECH, Nanjing, China), and blocked with 5% bovine serum albumin. .. Primary antibodies against FTH1 (Sangon Biotech, Shanghai, China), GPX4 (Sangon Biotech, Shanghai, China), HO-1 (Proteintech, Wuhan, China), KEAP1 (Proteintech, Wuhan, China), NRF2 (Proteintech, Wuhan, China), SLC40A1 (Proteintech, Wuhan, China), SLC7A11 (Sangon Biotech, Shanghai, China), TFRC (Proteintech, Wuhan, China), and β-actin (Proteintech, Wuhan, China) were incubated with the membrane at 4 ◦C overnight. .. Following an hour of room temperature incubation with an HRP-conjugated goat anti-rabbit secondary antibody (Proteintech, Wuhan, China), protein bands were identified using a ChemiScope 5300 (Clinx Science Instruments Co., Ltd, Shanghai, China).

Article Title: Depletion of p75NTR in Schwann Cells Driven by Inflammation Mediates Cutaneous Pain in Psoriasis.
Article Snippet: Proteins were separated by SDS-PAGE, transferred to PVDF membranes (Millipore, #ISEQ00010), and blocked with 5% BSA in PBST (0.05% Tween20) for 1 h at room temperature. .. Membranes were incubated overnight at 4◦C with primary antibodies (Anti-S100B (1:1000, sc-393919, Santa Cruz), anti-SOX10(1:1000, sc-365692, Santa Cruz), anti-MBP (1:1000, sc-271524, Santa Cruz), anti- β3-Tubulin (1:1000, #5566, Cell Signaling Technology), anti-p75NTR(1:1000, ab52987, ABcam), anti-NGF(1:1000, A14216, Abclonal), antiGM130 (1:1000, #70767, Cell Signaling Technology), anti-IL-17A (1:1000, 13082-1-AP, Proteintech), anti-p-TrkA (1;1000, #9141, Cell Signaling Technology), anti-TrkA (1:1000, #2505, Cell Signaling Technology), anti-c-Fos (1:1000, #2250, Cell Signaling Technology), anti-p-ERK1/2 (1:1000, #4370, Cell Signaling Technology), anti-ERK1/2 (1:1000, #9102, Cell Signaling Technology), anti-p-AKT (1:2000, #4060, Cell Signaling Technology), antiAKT (1:1000, #9272, Cell Signaling Technology), anti-GAPDH (1:5000, 60004-1-Ig, Proteintech), anti-KEAP1(1:1000, 10503-2-AP, Proteintech), anti-Nrf2 (1:1000, #20733, Cell Signaling Technol- 15 of 18 e C om m ons L icense o a i a g b ( 4 T o a t G G S t w ( i e w r b w f a 4 C e E D m m ( C 4 A w d c u w i w e A Y Z 1 nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202523189 by IN A SP - N E PA L , W iley O nline L ibrary on [06/04/2026]. .. Dermal tissue was rinsed in DMEM, minced, and digested in 2 mg/mL collagenase in DMEM (5 mL) at 37◦C for 2.5 h with Advanced Science, 2026 e g r d c c w a i 1 c s β w c o 4 P c c t S t r N e a p c 4 C t m 1 g C a s β ( A w p o ( A c t ( w t A s C i p A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202523189 by IN A SP - N E PA L , on [06/04/2026]

Recombinant:

Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
Article Snippet: .. Materials and methods 336 4.1 Reagents and antibodies 337 Reagents and antibodies used in this study were obtained from the indicated sources: 338 Recombinant human IL-2 (SL Pharm, S19991010); Annexin V-FITC/PI Apoptosis Kit 339 (Elabscience, E-CK-A211); Actinomycin D (MCE, HY-17559 ); TrueCut Cas9 340 Protein (Invitrogen, A36498); Nuclear and Cytoplasmic Extraction Reagents 341 (Thermo, 78835); Zombie Violet Fixable Viability Kit (BioLegend, 423113); 342 Pacific Blue anti-mouse CD3 Antibody (BioLegend, 100214); FITC anti-mouse CD8a 343 Antibody (BioLegend, 100706); APC anti-mouse CD8a Antibody (BD Pharmingen, 344 553035); PE/Cyanine7 anti-mouse CD3 Antibody (BioLegend, 100220); APC anti-345 mouse IFN-γ Antibody (Invivogen, 17-7311-82); PE-Labeled Mouse H-346 2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (Acrobiosystems, H2A-MP2H7); 347 Capicua Antibody (Novusbio, NB110-59906); anti-FLAG Antibody (Sigma-Aldrich, 348 F3165); β-actin (Sigma-Aldrich, A2228); Bim Antibody (Cell Signaling Technology, 349 2933T); Bcl-2 Antibody (Cell Signaling Technology, 3498T); Bax Antibody (Cell 350 Signaling Technology, 2772T); Bcl2 antibody (Proteintech, 68103-1-Ig); BAX 351 antibody (Proteintech, 60267-1-Ig); KEAP1 antibody (Proteintech, 10503-2-AP); 352 Anti-mouse Ig HRP (Rockland Immunochemicals, 18-8817-33); Anti-rabbit IgG HRP 353 (Rockland Immunochemicals, 18-8816-33); HRP-conjugated Mouse anti-Rabbit IgG 354 Light Chain (ABclonal, AS061); Prestained Protein Ladder 10-180 kDa (Thermo, 355 26616); Prestained Protein Ladder 25-400 kDa (Sevenbio, SW179). .. 356 4.2 Cells 357 CTLL-2 cells were purchased from the Cell Resource Center, Peking Union Medical 358 College (IBMS, CAMS/PUMC) and cultured in RPMI-1640 (Gibco) supplemented 359 with 10% fetal bovine serum (FBS) and 1× penicillin–streptomycin (Invitrogen), 10 360 mM HEPES, 1 mM Sodium pyruvate (Gibco), 2mM GlutaMAX (Gibco), and 100 361 U/ml IL-2.

Extraction:

Cell-Signaling:

Blocking Assay:

Membrane:

Hemagglutination Assay:

Article Title: Hepatocyte OTUD1 deubiquitinates and activates ERK1/2 to promote acetaminophen-induced liver injury.
Article Snippet: Acetaminophen (APAP)-induced liver injury is one of the most common causes of liver failure.. However, the critical role of deubiquitinating enzymes (DUBs) in APAP-induced hepatotoxicity remains unknown.. This study elucidated for the first time the molecular mechanisms by which OUT deubiquitinase-1 (OTUD1) regulates APAP-hepatoxicity.

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