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Beijing Genomics Institute Shenzhen itraq-based proteomic analysis
Itraq Based Proteomic Analysis, supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/itraq-based+proteomic+analysis/itraq+analysis/pmc10993572-153-2-7
Average 90 stars, based on 1 article reviews
itraq-based proteomic analysis - by Bioz Stars, 2026-09
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Article Title: Periostin promotes extensive neovascularization in placenta accreta spectrum disorders via Notch signaling.
Article Snippet: .. Protein preparation, quality control, and mass spectrometry characterization Protein samples (10 lg) harvested from the human placenta (n1⁄4 7) were solubilized, separated by sodium dodecyl sulfate–polyacrylamide electrophoresis (SDSPAGE, 12%), and subjected to iTRAQ analysis conducted by the Beijing Genomics Institute (China), as previously reported [12]. ..

Mass Spectrometry:

Article Title: Periostin promotes extensive neovascularization in placenta accreta spectrum disorders via Notch signaling.
Article Snippet: .. Protein preparation, quality control, and mass spectrometry characterization Protein samples (10 lg) harvested from the human placenta (n1⁄4 7) were solubilized, separated by sodium dodecyl sulfate–polyacrylamide electrophoresis (SDSPAGE, 12%), and subjected to iTRAQ analysis conducted by the Beijing Genomics Institute (China), as previously reported [12]. ..

Electrophoresis:

Article Title: Periostin promotes extensive neovascularization in placenta accreta spectrum disorders via Notch signaling.
Article Snippet: .. Protein preparation, quality control, and mass spectrometry characterization Protein samples (10 lg) harvested from the human placenta (n1⁄4 7) were solubilized, separated by sodium dodecyl sulfate–polyacrylamide electrophoresis (SDSPAGE, 12%), and subjected to iTRAQ analysis conducted by the Beijing Genomics Institute (China), as previously reported [12]. ..

Multiplex sample analysis:

Article Title: Periostin promotes extensive neovascularization in placenta accreta spectrum disorders via Notch signaling.
Article Snippet: .. Protein preparation, quality control, and mass spectrometry characterization Protein samples (10 lg) harvested from the human placenta (n1⁄4 7) were solubilized, separated by sodium dodecyl sulfate–polyacrylamide electrophoresis (SDSPAGE, 12%), and subjected to iTRAQ analysis conducted by the Beijing Genomics Institute (China), as previously reported [12]. ..

Article Title: Molecular Evidence that Lysiphlebia japonica Regulates the Development and Physiological Metabolism of Aphis gossypii
Article Snippet: .. We used iTRAQ analyses from the Beijing Genomics Institute, Shenzhen, China in order to study the protein profiles of parasitized and non-parasitized aphids. ..

Article Title: Therapy-induced senescent tumor cell-derived extracellular vesicles promote colorectal cancer progression through SERPINE1-mediated NF-κB p65 nuclear translocation
Article Snippet: .. The iTRAQ-based proteomic analysis was conducted by Beijing Genomics Institute. .. Briefly, proteins (100 μg) of each sample were digested using trypsin (1:20 w/w, Promega, Madison, USA) at 37 °C for 4 h. The digested protein peptide was desalted and labeled with iTRAQ reagents according to the kit protocol (Applied Biosystems, Foster City, USA).

Article Title: Exosomes derived from olive flounders infected with Streptococcus parauberis: Proteomic analysis, immunomodulation, and disease resistance capacity.
Article Snippet: Multidrug-resistant Streptococcus parauberis causes high fish mortality in aquaculture, necessitating an urgent need for innovative control strategies.. This study aimed to develop an immunizing agent against S. parauberis using exosomes isolated from the plasma of olive flounders infected experimentally with S. parauberis (Sp-Exo).. Initially, we tested the in vitro immunomodulatory effect of Sp-Exo in murine macrophage RAW264.7 cells and compared it to that of exosomes isolated from naïve fish (PBS-Exo-treated).

Article Title: Integrative Proteomic and MicroRNA Analysis: Insights Into Mechanisms of Eyestalk Ablation-Induced Ovarian Maturation in the Swimming Crab Portunus trituberculatus
Article Snippet: .. iTRAQ analysis was performed at Beijing Genomics Institute (BGI, Shenzhen, China). .. The ovary of each crab from the ESI and ESA groups were disrupted in lysis buffer with enzyme inhibitors by TissueLyser (Qiagen, USA).

Article Title: Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40)
Article Snippet: .. Furthermore, iTRAQ screening of the saphenous nerve axoplasm was performed by the Beijing Genomics institution, as published previously. .. Proteins from each sample were labeled with the iTRAQ reagent (Applied Biosystems) as follows: sample Y1-119 tags, Sample Y2-121 tags.

Article Title: Proteomics analysis reveals that the proto-oncogene eIF-5A indirectly influences the growth, invasion and replication of Toxoplasma gondii tachyzoite
Article Snippet: .. Isobaric labeling for relative and absolute quantitation (iTRAQ) analysis and bioinformatics analysis of proteins was carried out at Beijing Genomics Institute (BGI, Shenzhen, China). ..

Quantitation Assay:

Article Title: Exosomes derived from olive flounders infected with Streptococcus parauberis: Proteomic analysis, immunomodulation, and disease resistance capacity.
Article Snippet: Multidrug-resistant Streptococcus parauberis causes high fish mortality in aquaculture, necessitating an urgent need for innovative control strategies.. This study aimed to develop an immunizing agent against S. parauberis using exosomes isolated from the plasma of olive flounders infected experimentally with S. parauberis (Sp-Exo).. Initially, we tested the in vitro immunomodulatory effect of Sp-Exo in murine macrophage RAW264.7 cells and compared it to that of exosomes isolated from naïve fish (PBS-Exo-treated).

Article Title: Proteomics analysis reveals that the proto-oncogene eIF-5A indirectly influences the growth, invasion and replication of Toxoplasma gondii tachyzoite
Article Snippet: .. Isobaric labeling for relative and absolute quantitation (iTRAQ) analysis and bioinformatics analysis of proteins was carried out at Beijing Genomics Institute (BGI, Shenzhen, China). ..

Labeling:

Article Title: Proteomics analysis reveals that the proto-oncogene eIF-5A indirectly influences the growth, invasion and replication of Toxoplasma gondii tachyzoite
Article Snippet: .. Isobaric labeling for relative and absolute quantitation (iTRAQ) analysis and bioinformatics analysis of proteins was carried out at Beijing Genomics Institute (BGI, Shenzhen, China). ..

other:

Article Title: Comparative transcriptome and proteome reveal the unique genes and proteins of female parasitic wasps, Lysiphlebia japonica Ashmead.
Article Snippet: BACKGROUND: Lysiphlebia japonica Ashmead (Hymenoptera, Braconidae) is an endophagous parasitoid wasp and its host, Aphis gossypii Glover (Hemiptera, Aphididae) is a major cotton pest.. L. japonica affects the growth and fatty acid metabolism of cotton aphids after parasitization and has been widely used as a biocontrol agent.. However, there are currently few reports about the molecular characteristics of L. japonica, especially the differences between male and female.



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(A,B) iTRAQ-based <t>proteomics</t> analysis. (A) Apoptosis promotors Casp9 , Casp8 , Bak1, and Dap were significantly down-regulated by YKL-40, while the apoptosis inhibitor Aven was significantly up-regulated. (B) Volcano Plot . Molecules down-regulated by YKL-40 are presented in the upper left area (green down arrow) while those up-regulated are shown in the upper right area (red up arrow), and those involved in apoptosis regulation are marked with up/down solid arrows. The most significant fold change ( FC ) on the expression level of these molecules was caused by Casp9 ( FC = 0.6411), followed by Aven ( FC = 1.3006). (C,D) Caspase-9 expression levels detected in BMDM and aorta tissues of Ldlr −/- mice. YKL-40 significantly down-regulated the activation level of caspase-9. (E) In RAW264.7, which was upregulated by Ykl-40 , the activation level of caspase-9 was significantly lower than that in control group ( p = 0.0054) while the expression level of Aven was significantly higher than in controls ( p = 0.0031). There was no significant difference in caspase-9 activation and Aven expression level between the Ykl40 downregulated group and normal controls ( p > 0.05). (F) Genetic expression relative fold changes of Casp9 , Dap , Aven and Bak1. No significant difference was indicated on Casp9 in BMDM after being treated by YKL-40 recombinant protein, but Aven was significantly up-regulated. (G) Casp9 , Aven expression levels were significantly upregulated in Ykl40 upregulated RAW264.7 ( p = 0.0154, p < 0.0001) and downregulated in the Ykl40 downregulated group ( p = 0.0039, p = 0.0037).
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(A,B) iTRAQ-based proteomics analysis. (A) Apoptosis promotors Casp9 , Casp8 , Bak1, and Dap were significantly down-regulated by YKL-40, while the apoptosis inhibitor Aven was significantly up-regulated. (B) Volcano Plot . Molecules down-regulated by YKL-40 are presented in the upper left area (green down arrow) while those up-regulated are shown in the upper right area (red up arrow), and those involved in apoptosis regulation are marked with up/down solid arrows. The most significant fold change ( FC ) on the expression level of these molecules was caused by Casp9 ( FC = 0.6411), followed by Aven ( FC = 1.3006). (C,D) Caspase-9 expression levels detected in BMDM and aorta tissues of Ldlr −/- mice. YKL-40 significantly down-regulated the activation level of caspase-9. (E) In RAW264.7, which was upregulated by Ykl-40 , the activation level of caspase-9 was significantly lower than that in control group ( p = 0.0054) while the expression level of Aven was significantly higher than in controls ( p = 0.0031). There was no significant difference in caspase-9 activation and Aven expression level between the Ykl40 downregulated group and normal controls ( p > 0.05). (F) Genetic expression relative fold changes of Casp9 , Dap , Aven and Bak1. No significant difference was indicated on Casp9 in BMDM after being treated by YKL-40 recombinant protein, but Aven was significantly up-regulated. (G) Casp9 , Aven expression levels were significantly upregulated in Ykl40 upregulated RAW264.7 ( p = 0.0154, p < 0.0001) and downregulated in the Ykl40 downregulated group ( p = 0.0039, p = 0.0037).

Journal: Frontiers in Cell and Developmental Biology

Article Title: YKL-40 Aggravates Early-Stage Atherosclerosis by Inhibiting Macrophage Apoptosis in an Aven-dependent Way

doi: 10.3389/fcell.2021.752773

Figure Lengend Snippet: (A,B) iTRAQ-based proteomics analysis. (A) Apoptosis promotors Casp9 , Casp8 , Bak1, and Dap were significantly down-regulated by YKL-40, while the apoptosis inhibitor Aven was significantly up-regulated. (B) Volcano Plot . Molecules down-regulated by YKL-40 are presented in the upper left area (green down arrow) while those up-regulated are shown in the upper right area (red up arrow), and those involved in apoptosis regulation are marked with up/down solid arrows. The most significant fold change ( FC ) on the expression level of these molecules was caused by Casp9 ( FC = 0.6411), followed by Aven ( FC = 1.3006). (C,D) Caspase-9 expression levels detected in BMDM and aorta tissues of Ldlr −/- mice. YKL-40 significantly down-regulated the activation level of caspase-9. (E) In RAW264.7, which was upregulated by Ykl-40 , the activation level of caspase-9 was significantly lower than that in control group ( p = 0.0054) while the expression level of Aven was significantly higher than in controls ( p = 0.0031). There was no significant difference in caspase-9 activation and Aven expression level between the Ykl40 downregulated group and normal controls ( p > 0.05). (F) Genetic expression relative fold changes of Casp9 , Dap , Aven and Bak1. No significant difference was indicated on Casp9 in BMDM after being treated by YKL-40 recombinant protein, but Aven was significantly up-regulated. (G) Casp9 , Aven expression levels were significantly upregulated in Ykl40 upregulated RAW264.7 ( p = 0.0154, p < 0.0001) and downregulated in the Ykl40 downregulated group ( p = 0.0039, p = 0.0037).

Article Snippet: iTRAQ-based proteomics analysis (Genechem Co.,Ltd., Shanghai, China) were performed to screen out the potential downstream target molecules of YKL-40.

Techniques: Expressing, Activation Assay, Recombinant