iscript1tm cdna synthesis kit (Bio-Rad)
99
Structured Review
Bio-Rad
iscript1tm cdna synthesis kit
Iscript1tm Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript/iScript+cDNA+Synthesis+Kit/pmc12993172-136-9-13
Average 99 stars, based on 48025 article reviews
Iscript1tm Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript/iScript+cDNA+Synthesis+Kit/pmc12993172-136-9-13
Average 99 stars, based on 48025 article reviews
iscript1tm cdna synthesis kit - by Bioz Stars,
2026-10
99/100 stars
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Synthesized:Article Title: GPCR antagonism via rewiring of receptor trafficking and degradation Article Snippet: Total RNA was isolated from the ear skin, 24 h after the last IMQ application, using Trizol (Invitrogen) and RNeasy kits (Qiagen). .. cDNA was synthesized using oligo dT ( Article Title: E. coli O157:H7 evades CD8 + T cell immunity through T3SS-dependent suppression and limited MHCI presentation of translocated proteins. Article Snippet: .. RNA was extracted from EBL cells using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), and cDNA was synthesized using the Article Title: Svep1 orchestrates distal airway patterning and alveolar differentiation in murine lung development Article Snippet: Total RNA was isolated from whole embryonic lungs at E18.5 using the RNeasy Mini Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. Double-stranded cDNA was synthesized from total mouse lung RNA using the Article Title: Divergence Between Marker Expression and Phenotype During Human Corneal Endothelial Cell Expansion. Article Snippet: RNA concentration and purity were assessed on a BioDrop μLITE+ spectrophotometer (Biochrom, Cambridge, UK). .. Complementary DNA (cDNA) was synthesized from Article Title: Increased group 2 Innate lymphoid cell (ILC2) activity in pediatric familial mediterranean fever patients with spondyloarthropathy. Article Snippet: RNA isolation was performed with Invitrogen RNA Mini Kit following the manufacturer’s instructions. .. cDNA was synthesized with cDNA Synthesis:Article Title: GPCR antagonism via rewiring of receptor trafficking and degradation Article Snippet: Total RNA was isolated from the ear skin, 24 h after the last IMQ application, using Trizol (Invitrogen) and RNeasy kits (Qiagen). .. cDNA was synthesized using oligo dT ( Article Title: Protective Effects of the Phytocystatin CaneCPI-5 on Periodontal Ligament Cell Osteogenic Activity and Alveolar Bone Loss under Inflammatory Challenge Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions, and RNA concentration was determined with a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Subsequently, 500 ng of total RNA were reverse-transcribed into cDNA using the Article Title: E. coli O157:H7 evades CD8 + T cell immunity through T3SS-dependent suppression and limited MHCI presentation of translocated proteins. Article Snippet: .. RNA was extracted from EBL cells using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), and cDNA was synthesized using the Article Title: A neuronal GPCR modifies neuropeptide signaling to suppress proteostasis in distal tissues Article Snippet: The RNA was quantified using a NanoDrop 2000c spectrophotometer. .. For qPCR: cDNA was prepared by reverse transcription of the total RNA samples via random-priming using the Article Title: Svep1 orchestrates distal airway patterning and alveolar differentiation in murine lung development Article Snippet: Total RNA was isolated from whole embryonic lungs at E18.5 using the RNeasy Mini Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. Double-stranded cDNA was synthesized from total mouse lung RNA using the Article Title: Prospero prevents lineage-specific loss and gain by regulating cyclin E and mitosis during Drosophila neurogenesis. Article Snippet: Reagents and tools table Reagent/resource Source Identifier or catalog number Drosophila lines Df(3R) Exel7308 (prosdf) Bloomington Drosophila Stock Center (BDSC) BDSC_7959 w[*]; pros[17]/TM6B, Tb[1] (pros17) BDSC BDSC_5458 TM3, Kr-GAL4, UAS-GFP, Sb BDSC BDSC_5195 w[1118]; P{w[+mC]=UASGFP.nls}14 BDSC BDSC_4775 w[*]; P{w[+mW.hs]=GAL4wg.M}MA1 BDSC BDSC_4918 UAS-GFP-nls/CyO; prosdf, wgGAL4/TM3Sb Generated in the lab by recombination NA Primary antibodies (dilution used) Anti- Actin (1:10,000) Abcam Cat# ab8227, RRID:AB_2305186 Anti-BP102 (1:10) Developmental Studies Hybridoma Bank (DHSB) AB_528099 Anti-Cyclin E (rat, 1:100) A gift from Helena E. Richardson NA Anti-Cyclin E (mouse, 1:4) DHSB NA Anti-cleaved Drosophila Dcp-1 (Asp216) (1:200) Cell Signaling Technology Cat# 9578, RRID:AB_2721060 Anti-Dpn (1:100) Abcam Cat# ab195173, RRID:AB_2687586 Anti-FasII DHSB 1D4 RRID:AB_528235 Anti-Eagle (rabbit,1:2000) A gift from Chris Q. Doe NA Anti-Elav (mouse, IHC-1:5, Western-1:1000) DHSB 9F8A9 AB_528217 Anti-Elav (rat, 1:2000) DSHB 7E8A10 AB_528218 Anti-En/Inv (1:3) DSHB 4D9 AB_528224 Anti-Eve (1:2000) A gift from Manfred Frasch RRID:AB_2892600 Anti-GFP (rabbit, IHC-1:400, Western-1:10,000) Proteintech Cat# 50430-2-AP RRID:AB_11042881 Anti-GFP (chicken, IHC-1:200) Novus Cat# NB100-1614 RRID:AB_10001164 Anti-Miranda (IHC-1:100, Western-1:1000) Abcam Cat # ab197788 RRID: AB_2936368 Anti-Numb (Guinea Pig, 1:100) A gift from G. M. Technau N/A Reagent/resource Source Identifier or catalog number Anti- Phospho-Histone H3 (Ser10) (PH3) (rabbit, IHC-1:100, Western-1:1000) Cell Signaling Technology Cat # 9701 RRID:AB_331535 Anti-PH3 (mouse, 1:100) Cell Signaling Technology Cat # 9706 RRID:AB_331748 Anti-Pros (Mouse, 1:3) DSHB MR1A RRID:AB_528440 Anti-Repo (rabbit, 1:100). .. A gift from Dr. Benjamin Altenhein N/A Anti-Tubulin (rabbit, 1:10,000) Thermo Fisher Scientific Cat# MA5-27889 RRID:AB_2744943 Anti-Wg (mouse, 1:5) DSHB 4D4 RRID:AB_528512 Secondary Antibodies (dilution used) Goat anti-Mouse-Alkaline phosphatase (1:200) Jackson ImmunoResearch Cat # 115-055-146 RRID: AB_2338538 Goat anti-Mouse-HRP (IHC1:200, Western-1:10,000) Jackson ImmunoResearch Cat # 115-035-146 RRID: AB_2307392 Goat anti-Rabbit -Alexa Fluor 488 (1:300) Thermo Fisher Scientific Cat # A-11008 RRID: AB_143165 Goat anti-Mouse-Alexa Fluor 488 (1:300) Thermo Fisher Scientific Cat # A-1100 RRID: AB_2534069 Goat anti-Mouse-Alexa Fluor 594 (1:300) Thermo Fisher Scientific Cat # A-11005 RRID: AB_2534073 Goat anti-Rat-Alexa Fluor 594 (1:300) Thermo Fisher Scientific Cat # A-11007 RRID: AB_10561522 Goat anti-Chicken-Alexa Fluor 633 (1:300) Thermo Fisher Scientific Cat # A-21103 RRID: AB_2535756 Goat anti-Rabbit-Alexa Fluor 647 (1:300) Thermo Fisher Scientific Cat # A-21245 RRID: AB_2535813 Goat anti-mouse Alexa 647 (1:300) Thermo Fisher Scientific Cat # A-21236 RRID: AB_2535805 Goat Anti-Rat-HRP (1:10000) Jackson ImmunoResearch Cat # 112-035-062 RRID: AB_2338133 Donkey Anti-Rabbit-HRP (1:10000) Jackson ImmunoResearch Cat # 711-035-152 RRID: AB_10015282 Goat anti-Mouse-HRP (IHC1:200, Western 1:10000) Jackson ImmunoResearch Cat # 115-035-146 RRID: AB_2307392 Goat anti-Rabbit-HRP (1:10000) Thermo Fisher Scientific Cat # 31460 RRID: AB_228341 Kits Click-iT Plus TUNEL assay kit Thermo Fisher Scientific Cat # C10618 RNeasy Mini Kit Qiagen Cat # 74104 Article Title: Divergence Between Marker Expression and Phenotype During Human Corneal Endothelial Cell Expansion. Article Snippet: RNA concentration and purity were assessed on a BioDrop μLITE+ spectrophotometer (Biochrom, Cambridge, UK). .. Complementary DNA (cDNA) was synthesized from Article Title: Increased group 2 Innate lymphoid cell (ILC2) activity in pediatric familial mediterranean fever patients with spondyloarthropathy. Article Snippet: RNA isolation was performed with Invitrogen RNA Mini Kit following the manufacturer’s instructions. .. cDNA was synthesized with Reverse Transcription:Article Title: Protective Effects of the Phytocystatin CaneCPI-5 on Periodontal Ligament Cell Osteogenic Activity and Alveolar Bone Loss under Inflammatory Challenge Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions, and RNA concentration was determined with a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Subsequently, 500 ng of total RNA were reverse-transcribed into cDNA using the Article Title: A neuronal GPCR modifies neuropeptide signaling to suppress proteostasis in distal tissues Article Snippet: The RNA was quantified using a NanoDrop 2000c spectrophotometer. .. For qPCR: cDNA was prepared by reverse transcription of the total RNA samples via random-priming using the Real-time Polymerase Chain Reaction:Article Title: A neuronal GPCR modifies neuropeptide signaling to suppress proteostasis in distal tissues Article Snippet: The RNA was quantified using a NanoDrop 2000c spectrophotometer. .. For qPCR: cDNA was prepared by reverse transcription of the total RNA samples via random-priming using the Western Blot:Article Title: Prospero prevents lineage-specific loss and gain by regulating cyclin E and mitosis during Drosophila neurogenesis. Article Snippet: Reagents and tools table Reagent/resource Source Identifier or catalog number Drosophila lines Df(3R) Exel7308 (prosdf) Bloomington Drosophila Stock Center (BDSC) BDSC_7959 w[*]; pros[17]/TM6B, Tb[1] (pros17) BDSC BDSC_5458 TM3, Kr-GAL4, UAS-GFP, Sb BDSC BDSC_5195 w[1118]; P{w[+mC]=UASGFP.nls}14 BDSC BDSC_4775 w[*]; P{w[+mW.hs]=GAL4wg.M}MA1 BDSC BDSC_4918 UAS-GFP-nls/CyO; prosdf, wgGAL4/TM3Sb Generated in the lab by recombination NA Primary antibodies (dilution used) Anti- Actin (1:10,000) Abcam Cat# ab8227, RRID:AB_2305186 Anti-BP102 (1:10) Developmental Studies Hybridoma Bank (DHSB) AB_528099 Anti-Cyclin E (rat, 1:100) A gift from Helena E. Richardson NA Anti-Cyclin E (mouse, 1:4) DHSB NA Anti-cleaved Drosophila Dcp-1 (Asp216) (1:200) Cell Signaling Technology Cat# 9578, RRID:AB_2721060 Anti-Dpn (1:100) Abcam Cat# ab195173, RRID:AB_2687586 Anti-FasII DHSB 1D4 RRID:AB_528235 Anti-Eagle (rabbit,1:2000) A gift from Chris Q. Doe NA Anti-Elav (mouse, IHC-1:5, Western-1:1000) DHSB 9F8A9 AB_528217 Anti-Elav (rat, 1:2000) DSHB 7E8A10 AB_528218 Anti-En/Inv (1:3) DSHB 4D9 AB_528224 Anti-Eve (1:2000) A gift from Manfred Frasch RRID:AB_2892600 Anti-GFP (rabbit, IHC-1:400, Western-1:10,000) Proteintech Cat# 50430-2-AP RRID:AB_11042881 Anti-GFP (chicken, IHC-1:200) Novus Cat# NB100-1614 RRID:AB_10001164 Anti-Miranda (IHC-1:100, Western-1:1000) Abcam Cat # ab197788 RRID: AB_2936368 Anti-Numb (Guinea Pig, 1:100) A gift from G. M. Technau N/A Reagent/resource Source Identifier or catalog number Anti- Phospho-Histone H3 (Ser10) (PH3) (rabbit, IHC-1:100, Western-1:1000) Cell Signaling Technology Cat # 9701 RRID:AB_331535 Anti-PH3 (mouse, 1:100) Cell Signaling Technology Cat # 9706 RRID:AB_331748 Anti-Pros (Mouse, 1:3) DSHB MR1A RRID:AB_528440 Anti-Repo (rabbit, 1:100). .. A gift from Dr. Benjamin Altenhein N/A Anti-Tubulin (rabbit, 1:10,000) Thermo Fisher Scientific Cat# MA5-27889 RRID:AB_2744943 Anti-Wg (mouse, 1:5) DSHB 4D4 RRID:AB_528512 Secondary Antibodies (dilution used) Goat anti-Mouse-Alkaline phosphatase (1:200) Jackson ImmunoResearch Cat # 115-055-146 RRID: AB_2338538 Goat anti-Mouse-HRP (IHC1:200, Western-1:10,000) Jackson ImmunoResearch Cat # 115-035-146 RRID: AB_2307392 Goat anti-Rabbit -Alexa Fluor 488 (1:300) Thermo Fisher Scientific Cat # A-11008 RRID: AB_143165 Goat anti-Mouse-Alexa Fluor 488 (1:300) Thermo Fisher Scientific Cat # A-1100 RRID: AB_2534069 Goat anti-Mouse-Alexa Fluor 594 (1:300) Thermo Fisher Scientific Cat # A-11005 RRID: AB_2534073 Goat anti-Rat-Alexa Fluor 594 (1:300) Thermo Fisher Scientific Cat # A-11007 RRID: AB_10561522 Goat anti-Chicken-Alexa Fluor 633 (1:300) Thermo Fisher Scientific Cat # A-21103 RRID: AB_2535756 Goat anti-Rabbit-Alexa Fluor 647 (1:300) Thermo Fisher Scientific Cat # A-21245 RRID: AB_2535813 Goat anti-mouse Alexa 647 (1:300) Thermo Fisher Scientific Cat # A-21236 RRID: AB_2535805 Goat Anti-Rat-HRP (1:10000) Jackson ImmunoResearch Cat # 112-035-062 RRID: AB_2338133 Donkey Anti-Rabbit-HRP (1:10000) Jackson ImmunoResearch Cat # 711-035-152 RRID: AB_10015282 Goat anti-Mouse-HRP (IHC1:200, Western 1:10000) Jackson ImmunoResearch Cat # 115-035-146 RRID: AB_2307392 Goat anti-Rabbit-HRP (1:10000) Thermo Fisher Scientific Cat # 31460 RRID: AB_228341 Kits Click-iT Plus TUNEL assay kit Thermo Fisher Scientific Cat # C10618 RNeasy Mini Kit Qiagen Cat # 74104 TUNEL Assay:Article Title: Prospero prevents lineage-specific loss and gain by regulating cyclin E and mitosis during Drosophila neurogenesis. Article Snippet: Reagents and tools table Reagent/resource Source Identifier or catalog number Drosophila lines Df(3R) Exel7308 (prosdf) Bloomington Drosophila Stock Center (BDSC) BDSC_7959 w[*]; pros[17]/TM6B, Tb[1] (pros17) BDSC BDSC_5458 TM3, Kr-GAL4, UAS-GFP, Sb BDSC BDSC_5195 w[1118]; P{w[+mC]=UASGFP.nls}14 BDSC BDSC_4775 w[*]; P{w[+mW.hs]=GAL4wg.M}MA1 BDSC BDSC_4918 UAS-GFP-nls/CyO; prosdf, wgGAL4/TM3Sb Generated in the lab by recombination NA Primary antibodies (dilution used) Anti- Actin (1:10,000) Abcam Cat# ab8227, RRID:AB_2305186 Anti-BP102 (1:10) Developmental Studies Hybridoma Bank (DHSB) AB_528099 Anti-Cyclin E (rat, 1:100) A gift from Helena E. Richardson NA Anti-Cyclin E (mouse, 1:4) DHSB NA Anti-cleaved Drosophila Dcp-1 (Asp216) (1:200) Cell Signaling Technology Cat# 9578, RRID:AB_2721060 Anti-Dpn (1:100) Abcam Cat# ab195173, RRID:AB_2687586 Anti-FasII DHSB 1D4 RRID:AB_528235 Anti-Eagle (rabbit,1:2000) A gift from Chris Q. Doe NA Anti-Elav (mouse, IHC-1:5, Western-1:1000) DHSB 9F8A9 AB_528217 Anti-Elav (rat, 1:2000) DSHB 7E8A10 AB_528218 Anti-En/Inv (1:3) DSHB 4D9 AB_528224 Anti-Eve (1:2000) A gift from Manfred Frasch RRID:AB_2892600 Anti-GFP (rabbit, IHC-1:400, Western-1:10,000) Proteintech Cat# 50430-2-AP RRID:AB_11042881 Anti-GFP (chicken, IHC-1:200) Novus Cat# NB100-1614 RRID:AB_10001164 Anti-Miranda (IHC-1:100, Western-1:1000) Abcam Cat # ab197788 RRID: AB_2936368 Anti-Numb (Guinea Pig, 1:100) A gift from G. M. Technau N/A Reagent/resource Source Identifier or catalog number Anti- Phospho-Histone H3 (Ser10) (PH3) (rabbit, IHC-1:100, Western-1:1000) Cell Signaling Technology Cat # 9701 RRID:AB_331535 Anti-PH3 (mouse, 1:100) Cell Signaling Technology Cat # 9706 RRID:AB_331748 Anti-Pros (Mouse, 1:3) DSHB MR1A RRID:AB_528440 Anti-Repo (rabbit, 1:100). .. A gift from Dr. Benjamin Altenhein N/A Anti-Tubulin (rabbit, 1:10,000) Thermo Fisher Scientific Cat# MA5-27889 RRID:AB_2744943 Anti-Wg (mouse, 1:5) DSHB 4D4 RRID:AB_528512 Secondary Antibodies (dilution used) Goat anti-Mouse-Alkaline phosphatase (1:200) Jackson ImmunoResearch Cat # 115-055-146 RRID: AB_2338538 Goat anti-Mouse-HRP (IHC1:200, Western-1:10,000) Jackson ImmunoResearch Cat # 115-035-146 RRID: AB_2307392 Goat anti-Rabbit -Alexa Fluor 488 (1:300) Thermo Fisher Scientific Cat # A-11008 RRID: AB_143165 Goat anti-Mouse-Alexa Fluor 488 (1:300) Thermo Fisher Scientific Cat # A-1100 RRID: AB_2534069 Goat anti-Mouse-Alexa Fluor 594 (1:300) Thermo Fisher Scientific Cat # A-11005 RRID: AB_2534073 Goat anti-Rat-Alexa Fluor 594 (1:300) Thermo Fisher Scientific Cat # A-11007 RRID: AB_10561522 Goat anti-Chicken-Alexa Fluor 633 (1:300) Thermo Fisher Scientific Cat # A-21103 RRID: AB_2535756 Goat anti-Rabbit-Alexa Fluor 647 (1:300) Thermo Fisher Scientific Cat # A-21245 RRID: AB_2535813 Goat anti-mouse Alexa 647 (1:300) Thermo Fisher Scientific Cat # A-21236 RRID: AB_2535805 Goat Anti-Rat-HRP (1:10000) Jackson ImmunoResearch Cat # 112-035-062 RRID: AB_2338133 Donkey Anti-Rabbit-HRP (1:10000) Jackson ImmunoResearch Cat # 711-035-152 RRID: AB_10015282 Goat anti-Mouse-HRP (IHC1:200, Western 1:10000) Jackson ImmunoResearch Cat # 115-035-146 RRID: AB_2307392 Goat anti-Rabbit-HRP (1:10000) Thermo Fisher Scientific Cat # 31460 RRID: AB_228341 Kits Click-iT Plus TUNEL assay kit Thermo Fisher Scientific Cat # C10618 RNeasy Mini Kit Qiagen Cat # 74104 |