iscripttm cdna synthesis kit (Bio-Rad)
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iscripttm cdna synthesis kit
Iscripttm Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript/iScript+cDNA+Synthesis+Kit/pm42905847-445-16-20
Average 99 stars, based on 48597 article reviews
Iscripttm Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript/iScript+cDNA+Synthesis+Kit/pm42905847-445-16-20
Average 99 stars, based on 48597 article reviews
iscripttm cdna synthesis kit - by Bioz Stars,
2026-11
99/100 stars
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Synthesized:Article Title: Permanent Senescence Via p16 Leads to Guttae Formation in an In Vitro Human Corneal Endothelial Cell Model Article Snippet: The RNA samples were collected in nuclease-free water to measure the RNA quality and quantity using NanoDrop (Thermo Fisher, Waltham, MA, USA). .. Complimentary DNA (cDNA) was synthesized by Chloramphenicol Acetyltransferase Assay:Article Title: Central relaxin-3/RXFP3 signaling potently drives orexigenic behaviors and attenuates cisplatin-induced anorexia and body weight loss. Article Snippet: Adult wildtype (∼8 weeks old) maleSprague-Dawley rats (Charles River, RRID:RGD_734476) weighing ∼250–275 upon arrival were individually housed under a 12h-light:12h-dark cycle in a temperature and humidity-controlled satellite vivarium and had ad libitum access to water and chow (5001, LabDiet; Calories provided by protein 28.9%, fat 13.6%, carbohydrate 57.5%; 3.35 kcal/g) or a 60% high fat diet (HFD; D12492, Research diets; Calories provided by protein 26.2%, fat 34.9%, carbohydrate 26.3%; 5.24 kcal/g) and when indicated had ad libitum access to kaolin pellets (Research Diets, K50001) to assess pica behavior. .. Rats were exposed to kaolin REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Mouse brain (Rxfp3 fl/fl hsyn-Cre+) In house (University of Pennsylvania) N/A Mouse brain (Rxfp3 fl/fl hsyn-Cre-) In house (University of Pennsylvania) CAT JAX:028543 Mouse brain (hSyn-Cre+) In house (University of Pennsylvania) CAT# JAX:003966 Chemicals, peptides, and recombinant proteins Relaxin-3 Phoenix Pharmaceuticals CAT# 035–36 Cisplatin Sigma Aldrich CAT# 15663–27-1 GDF-15 Abcam ab302451 LiCl DMSO Honeywell CAT# 34869-1L Artificial cerebrospinal fluid (aCSF) Havard Appartus CAT# 59–7316 Angiotensin II (Ang II) Bachem Product #4006473 Critical commercial assays Fast Advanced Master Mix Applied Biosystems CAT# 4444554 Recombinant:Article Title: Central relaxin-3/RXFP3 signaling potently drives orexigenic behaviors and attenuates cisplatin-induced anorexia and body weight loss. Article Snippet: Adult wildtype (∼8 weeks old) maleSprague-Dawley rats (Charles River, RRID:RGD_734476) weighing ∼250–275 upon arrival were individually housed under a 12h-light:12h-dark cycle in a temperature and humidity-controlled satellite vivarium and had ad libitum access to water and chow (5001, LabDiet; Calories provided by protein 28.9%, fat 13.6%, carbohydrate 57.5%; 3.35 kcal/g) or a 60% high fat diet (HFD; D12492, Research diets; Calories provided by protein 26.2%, fat 34.9%, carbohydrate 26.3%; 5.24 kcal/g) and when indicated had ad libitum access to kaolin pellets (Research Diets, K50001) to assess pica behavior. .. Rats were exposed to kaolin REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Mouse brain (Rxfp3 fl/fl hsyn-Cre+) In house (University of Pennsylvania) N/A Mouse brain (Rxfp3 fl/fl hsyn-Cre-) In house (University of Pennsylvania) CAT JAX:028543 Mouse brain (hSyn-Cre+) In house (University of Pennsylvania) CAT# JAX:003966 Chemicals, peptides, and recombinant proteins Relaxin-3 Phoenix Pharmaceuticals CAT# 035–36 Cisplatin Sigma Aldrich CAT# 15663–27-1 GDF-15 Abcam ab302451 LiCl DMSO Honeywell CAT# 34869-1L Artificial cerebrospinal fluid (aCSF) Havard Appartus CAT# 59–7316 Angiotensin II (Ang II) Bachem Product #4006473 Critical commercial assays Fast Advanced Master Mix Applied Biosystems CAT# 4444554 Generated:Article Title: Central relaxin-3/RXFP3 signaling potently drives orexigenic behaviors and attenuates cisplatin-induced anorexia and body weight loss. Article Snippet: Adult wildtype (∼8 weeks old) maleSprague-Dawley rats (Charles River, RRID:RGD_734476) weighing ∼250–275 upon arrival were individually housed under a 12h-light:12h-dark cycle in a temperature and humidity-controlled satellite vivarium and had ad libitum access to water and chow (5001, LabDiet; Calories provided by protein 28.9%, fat 13.6%, carbohydrate 57.5%; 3.35 kcal/g) or a 60% high fat diet (HFD; D12492, Research diets; Calories provided by protein 26.2%, fat 34.9%, carbohydrate 26.3%; 5.24 kcal/g) and when indicated had ad libitum access to kaolin pellets (Research Diets, K50001) to assess pica behavior. .. Rats were exposed to kaolin REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Mouse brain (Rxfp3 fl/fl hsyn-Cre+) In house (University of Pennsylvania) N/A Mouse brain (Rxfp3 fl/fl hsyn-Cre-) In house (University of Pennsylvania) CAT JAX:028543 Mouse brain (hSyn-Cre+) In house (University of Pennsylvania) CAT# JAX:003966 Chemicals, peptides, and recombinant proteins Relaxin-3 Phoenix Pharmaceuticals CAT# 035–36 Cisplatin Sigma Aldrich CAT# 15663–27-1 GDF-15 Abcam ab302451 LiCl DMSO Honeywell CAT# 34869-1L Artificial cerebrospinal fluid (aCSF) Havard Appartus CAT# 59–7316 Angiotensin II (Ang II) Bachem Product #4006473 Critical commercial assays Fast Advanced Master Mix Applied Biosystems CAT# 4444554 Software:Article Title: Central relaxin-3/RXFP3 signaling potently drives orexigenic behaviors and attenuates cisplatin-induced anorexia and body weight loss. Article Snippet: Adult wildtype (∼8 weeks old) maleSprague-Dawley rats (Charles River, RRID:RGD_734476) weighing ∼250–275 upon arrival were individually housed under a 12h-light:12h-dark cycle in a temperature and humidity-controlled satellite vivarium and had ad libitum access to water and chow (5001, LabDiet; Calories provided by protein 28.9%, fat 13.6%, carbohydrate 57.5%; 3.35 kcal/g) or a 60% high fat diet (HFD; D12492, Research diets; Calories provided by protein 26.2%, fat 34.9%, carbohydrate 26.3%; 5.24 kcal/g) and when indicated had ad libitum access to kaolin pellets (Research Diets, K50001) to assess pica behavior. .. Rats were exposed to kaolin REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Mouse brain (Rxfp3 fl/fl hsyn-Cre+) In house (University of Pennsylvania) N/A Mouse brain (Rxfp3 fl/fl hsyn-Cre-) In house (University of Pennsylvania) CAT JAX:028543 Mouse brain (hSyn-Cre+) In house (University of Pennsylvania) CAT# JAX:003966 Chemicals, peptides, and recombinant proteins Relaxin-3 Phoenix Pharmaceuticals CAT# 035–36 Cisplatin Sigma Aldrich CAT# 15663–27-1 GDF-15 Abcam ab302451 LiCl DMSO Honeywell CAT# 34869-1L Artificial cerebrospinal fluid (aCSF) Havard Appartus CAT# 59–7316 Angiotensin II (Ang II) Bachem Product #4006473 Critical commercial assays Fast Advanced Master Mix Applied Biosystems CAT# 4444554 other:Article Title: Immune regulators PALS-25 and PALS-22 localize to mitochondria and regulate mitochondrial fragmentation in C. elegans Article Snippet: 501 502 RNA extraction and RT-qPCR 503 For Fig. S3A,B: ~3000-3,500 synchronized L1s for ERT964 pals-22 pals-25(jy80); jyEx193[pals-504 22p::pals-22::GFP::3xFLAG] or ERT965 pals-22 pals-25(jy80); jyEx237[pals-22p::pals-505 25::GFP::3xFLAG] were plated to 10-cm RNAi plates seeded with 1 ml overnight culture of L4440 506 control vector or pals-22 RNAi clones and were incubated at 20 °C for 48 h. For Fig. S3C: ~1000 507 synchronized L1s of ERT714 pals-22 pals-25(jy80), ERT751 pals-25(jy111), ERT964 pals-22 508 pals-25(jy80) unc-119(ed3) III; jyEx193[PALS-22::EGFP::3xFLAG, unc-119(+)], and ERT965 509 pals-22 pals-25(jy80) unc-119(ed3) III; jyEx237[PALS-25::EGFP::3xFLAG, unc-119(+)] were 510 plated onto two separate 10-cm plates and incubated at 20 °C for 50 h. For all RT-qPCR 511 experiments, RNA was extracted with TRI reagent (Molecular Research Center TR118), isolated 512 with BCP reagent (Molecular Research Center BP151), washed with 100% isopropanol followed 513 by 75% ethanol, and resuspended in HyClone nuclease-free H2O (Cytiva SH30538.03). cDNA 514 was synthesized from total RNA using Article Title: ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage-like foam cells in atherosclerosis Article Snippet: Adhered macrophages were detached by incubation for 5 min at 37°C with pre-warmed, sterile Lidocaine solution [4 mg/mL Lidocaine (Merck, Darmstadt, Germany Cat. no. L5647-15G)/10 mM EDTA/PBS (without Ca2+ and Mg2+)] after having washed the cells with PBS. Article Title: Immune regulators PALS-25 and PALS-22 localize to mitochondria and regulate mitochondrial fragmentation in C. elegans Article Snippet: For A and S3B: ∼3000-3,500 synchronized L1s for ERT964 pals-22 pals-25(jy80); jyEx193[pals-22p::pals-22::GFP::3xFLAG] or ERT965 pals-22 pals-25(jy80); jyEx237[pals-22p::pals-25::GFP::3xFLAG] were plated to 10-cm RNAi plates seeded with 1 mL overnight culture of L4440 control vector or pals-22 RNAi clones and were incubated at 20 °C for 48 h. For C: ∼1000 synchronized L1s of ERT714 pals-22 pals-25(jy80) , ERT751 pals-25(jy111) , ERT964 pals-22 pals-25(jy80) unc-119(ed3) III; jyEx193[ pals -22:: GFP::3xFLAG, unc-119(+)] , and ERT965 pals-22 pals-25(jy80) unc-119(ed3) III ; jyEx237[ pals -25::EGFP::3xFLAG, unc-119(+)] were plated onto two separate 10-cm plates and incubated at 20 °C for 50 h. For all RT-qPCR experiments, RNA was extracted with TRI reagent (Molecular Research Center TR118), isolated with BCP reagent (Molecular Research Center BP151), washed with 100% isopropanol followed by 75% ethanol, and resuspended in HyClone nuclease-free H 2 O (Cytiva SH30538.03). cDNA was synthesized from total RNA using Isolation:Article Title: COMP-PMEPA1 axis promotes epithelial-to-mesenchymal transition in breast cancer cells. Article Snippet: The amplification was carried out using the Viia 7 real-time PCR system (Applied Biosystems). .. Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions. cDNA synthesis was performed using the cDNA Synthesis:Article Title: COMP-PMEPA1 axis promotes epithelial-to-mesenchymal transition in breast cancer cells. Article Snippet: The amplification was carried out using the Viia 7 real-time PCR system (Applied Biosystems). .. Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions. cDNA synthesis was performed using the Reverse Transcription:Article Title: COMP-PMEPA1 axis promotes epithelial-to-mesenchymal transition in breast cancer cells. Article Snippet: The amplification was carried out using the Viia 7 real-time PCR system (Applied Biosystems). .. Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions. cDNA synthesis was performed using the Article Title: ChemR23 prevents phenotypic switching of vascular smooth muscle cells into macrophage like foam cells in atherosclerosis. Article Snippet: RNA was isolated using the Promega RNA Miniprep (Z6011) Systems and 19 quantified using a NanoDropTM 2000/2000c spectrophotometer (ThermoFisher). .. RNA (200 ng) was then 20 converted to cDNA using a reverse transcriptase enzyme, |