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iscript cdna synthesis kit  (Bio-Rad)


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    Bio-Rad iscript cdna synthesis kit
    Iscript Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6662 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript/IScript+C+DNA+Synthesis+Kit/pm42814240-84-30-34
    Average 99 stars, based on 6662 article reviews
    iscript cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: In Vitro Generation of TGFb- and IL1a-Induced Fibroblast Subtypes for Mechanistic Studies of Extracellular Matrix Composition and Architecture
    Article Snippet: Lysates were passed 10 times through a sterile BD MicrolanceTM 3 needle (#300635, 27G 0.4 x 13 mm) attached to a 1 mL BD Luer-Lok Syringe (#309628) and RNA was further isolated using the protocol provided by the manufacturer. .. For cDNA, the iScriptTM cDNA Synthesis Kit (Bio-Rad, #1708891) was used. .. RTqPCR was executed with iQTM SYBR Green Supermix (BioRad, ##1708886) on the CFX96TM Touch Real-Time System.

    Article Title: Disruption of local CTCF binding impairs renin expression and blood pressure response in Dahl salt-sensitive rats.
    Article Snippet: 1 in Dahl salt-sensitive rats 2 Mark Vanden Avond, Michael N. Grzybowski, Terry Kurth, Chun Yang, Jason Klotz, Ana 3 Hantke Guixa, Andrew Greene, Sridhar Rao, Qiongzi Qiu, Pengyuan Liu 7, , Yong Liu, 4 Mingyu Liang, Allen W. Cowley Jr., Aron M. Geurts 5 Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA 6 The Jackson Laboratory for Mammalian Genetics, Bar Harbor, ME, USA 7 Department of Pediatrics, Division of Hematology, Oncology, and Bone Marrow 8 Transplantation, Medical College of Wisconsin, Milwaukee, WI, USA.. 9 Versiti Blood Research Institute, Milwaukee, WI, USA.. 10 Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 11 Milwaukee, WI, USA.

    Article Title: Salivary miRNAs along with p53 and EGFR in oral exfoliated cells: a pilot study on candidate noninvasive biomarkers for the early detection of oral cancer in tobacco users.
    Article Snippet: 1 Centre for Medical Biotechnology, Maharshi Dayanand University, Rohtak, Haryana 124001, India 2 Swami Shraddhanand College, University of Delhi, New Delhi 110036, India 3 Dept.. of Oral Pathology, Postgraduate Institute of Dental Sciences, Rohtak, Haryana 124001, India Abstract Background The low 5-year survival rate for Oral Squamous Cell Carcinoma (OSCC) is largely attributed to the limitations of current invasive diagnostic procedures and diagnosis at a late stage.. Biomarkers from saliva and exfoliated oral cells could be used to diagnose oral cancer as the malignancy begins in the epithelium and would be particularly useful for screening patients in high-risk categories like tobacco users.

    Article Title: Mitotic bypass and continued endocycling promote cancer cell survival after genotoxic chemotherapy
    Article Snippet: mRNA was isolated using Rneasy Plus kit (Qiagen #74134) and concentration was quantified with NanoDrop One (Thermo Scientific). .. Reverse transcription of 1 μg input mRNA was done using iScript cDNA synthesis kit (BioRad #1708890) according to manufacturer’s protocol. .. Real-time PCR was done using SssoFast EvaGreen (BioRad #1725200) and run on BioRad CFX Opus real-time PCR machine.

    Article Title: PLOD1 Catalytic Activity Stabilizes ENO1 by Limiting FBXW7-dependent Degradation to Promote Glycolysis and TMZ Resistance in Glioblastoma.
    Article Snippet: .8 RNA Extraction and Quantitative Real-Time CR (qRT-PCR) otal RNA was extracted using the RNeasy Mini Kit (Qiagen, 74104). .. cDNA was synthesized using the iScript cDNA Synthesis it (Bio-Rad, #1708891). .. qRT-PCR was performed on a CFX96 eal-Time PCR Detection System (Bio-Rad) using iTaq Universal YBR Green Supermix (Bio-Rad, #1725121).

    Article Title: Cyclic nucleotide signaling through phosphodiesterase 1 inhibition and its interaction with the competing endogenous RNA network regulates cognition.
    Article Snippet: Total RNA was extracted from the mouse brain with the RNeasy Mini Kit (Qiagen, Cat. No. 74104). .. Reverse transcription was conducted by iScript cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890) to generate cDNA for further mRNA and miRNA analysis. .. RT-qPCR was performed using custom primers and iTaq Universal SYBR Green Supermix (BioRad) on a Bio-Rad CFX Opus 96 Real-Time PCR System.

    Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
    Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).

    Real-time Polymerase Chain Reaction:

    Article Title: Disruption of local CTCF binding impairs renin expression and blood pressure response in Dahl salt-sensitive rats.
    Article Snippet: 1 in Dahl salt-sensitive rats 2 Mark Vanden Avond, Michael N. Grzybowski, Terry Kurth, Chun Yang, Jason Klotz, Ana 3 Hantke Guixa, Andrew Greene, Sridhar Rao, Qiongzi Qiu, Pengyuan Liu 7, , Yong Liu, 4 Mingyu Liang, Allen W. Cowley Jr., Aron M. Geurts 5 Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA 6 The Jackson Laboratory for Mammalian Genetics, Bar Harbor, ME, USA 7 Department of Pediatrics, Division of Hematology, Oncology, and Bone Marrow 8 Transplantation, Medical College of Wisconsin, Milwaukee, WI, USA.. 9 Versiti Blood Research Institute, Milwaukee, WI, USA.. 10 Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 11 Milwaukee, WI, USA.

    Article Title: Macrophages modulate ovarian cancer progression by regulating epithelial-mesenchymal transition.
    Article Snippet: The isolated RNA was dissolved in nuclease-free water and quantified using a NanoDrop spectrophotometer (Implen). .. cDNA synthesis was carried out using the iScript cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890) according to the manufacturer’s instructions under the following cycling conditions: 25 ◦C for 5 min, 46 ◦C for 20 min, and 95 ◦C for 5 min. Quantitative PCR was performed using SYBR Green Supermix (Bio-Rad, Cat. No. 171–5121) on the QuantStudio 5 Real-Time PCR System. .. The expression levels of M1 and M2 markers (CD206, CD163, ARG1, iNOS and CD86) whereas EMT-related genes (E-cadherin, N-cadherin, Snail, Slug, MMP-2, Twist, Vimentin, and ZEB) were analysed, with GAPDH serving as the endogenous control.

    Reverse Transcription:

    Article Title: Disruption of local CTCF binding impairs renin expression and blood pressure response in Dahl salt-sensitive rats.
    Article Snippet: 1 in Dahl salt-sensitive rats 2 Mark Vanden Avond, Michael N. Grzybowski, Terry Kurth, Chun Yang, Jason Klotz, Ana 3 Hantke Guixa, Andrew Greene, Sridhar Rao, Qiongzi Qiu, Pengyuan Liu 7, , Yong Liu, 4 Mingyu Liang, Allen W. Cowley Jr., Aron M. Geurts 5 Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA 6 The Jackson Laboratory for Mammalian Genetics, Bar Harbor, ME, USA 7 Department of Pediatrics, Division of Hematology, Oncology, and Bone Marrow 8 Transplantation, Medical College of Wisconsin, Milwaukee, WI, USA.. 9 Versiti Blood Research Institute, Milwaukee, WI, USA.. 10 Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 11 Milwaukee, WI, USA.

    Article Title: Mitotic bypass and continued endocycling promote cancer cell survival after genotoxic chemotherapy
    Article Snippet: mRNA was isolated using Rneasy Plus kit (Qiagen #74134) and concentration was quantified with NanoDrop One (Thermo Scientific). .. Reverse transcription of 1 μg input mRNA was done using iScript cDNA synthesis kit (BioRad #1708890) according to manufacturer’s protocol. .. Real-time PCR was done using SssoFast EvaGreen (BioRad #1725200) and run on BioRad CFX Opus real-time PCR machine.

    Article Title: Cyclic nucleotide signaling through phosphodiesterase 1 inhibition and its interaction with the competing endogenous RNA network regulates cognition.
    Article Snippet: Total RNA was extracted from the mouse brain with the RNeasy Mini Kit (Qiagen, Cat. No. 74104). .. Reverse transcription was conducted by iScript cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890) to generate cDNA for further mRNA and miRNA analysis. .. RT-qPCR was performed using custom primers and iTaq Universal SYBR Green Supermix (BioRad) on a Bio-Rad CFX Opus 96 Real-Time PCR System.

    Incubation:

    Article Title: Salivary miRNAs along with p53 and EGFR in oral exfoliated cells: a pilot study on candidate noninvasive biomarkers for the early detection of oral cancer in tobacco users.
    Article Snippet: 1 Centre for Medical Biotechnology, Maharshi Dayanand University, Rohtak, Haryana 124001, India 2 Swami Shraddhanand College, University of Delhi, New Delhi 110036, India 3 Dept.. of Oral Pathology, Postgraduate Institute of Dental Sciences, Rohtak, Haryana 124001, India Abstract Background The low 5-year survival rate for Oral Squamous Cell Carcinoma (OSCC) is largely attributed to the limitations of current invasive diagnostic procedures and diagnosis at a late stage.. Biomarkers from saliva and exfoliated oral cells could be used to diagnose oral cancer as the malignancy begins in the epithelium and would be particularly useful for screening patients in high-risk categories like tobacco users.

    Synthesized:

    Article Title: PLOD1 Catalytic Activity Stabilizes ENO1 by Limiting FBXW7-dependent Degradation to Promote Glycolysis and TMZ Resistance in Glioblastoma.
    Article Snippet: .8 RNA Extraction and Quantitative Real-Time CR (qRT-PCR) otal RNA was extracted using the RNeasy Mini Kit (Qiagen, 74104). .. cDNA was synthesized using the iScript cDNA Synthesis it (Bio-Rad, #1708891). .. qRT-PCR was performed on a CFX96 eal-Time PCR Detection System (Bio-Rad) using iTaq Universal YBR Green Supermix (Bio-Rad, #1725121).

    Analysis:

    Article Title: Cyclic nucleotide signaling through phosphodiesterase 1 inhibition and its interaction with the competing endogenous RNA network regulates cognition.
    Article Snippet: Total RNA was extracted from the mouse brain with the RNeasy Mini Kit (Qiagen, Cat. No. 74104). .. Reverse transcription was conducted by iScript cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890) to generate cDNA for further mRNA and miRNA analysis. .. RT-qPCR was performed using custom primers and iTaq Universal SYBR Green Supermix (BioRad) on a Bio-Rad CFX Opus 96 Real-Time PCR System.

    Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
    Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).

    SYBR Green Assay:

    Article Title: Macrophages modulate ovarian cancer progression by regulating epithelial-mesenchymal transition.
    Article Snippet: The isolated RNA was dissolved in nuclease-free water and quantified using a NanoDrop spectrophotometer (Implen). .. cDNA synthesis was carried out using the iScript cDNA Synthesis Kit (Bio-Rad, Cat. No. 1708890) according to the manufacturer’s instructions under the following cycling conditions: 25 ◦C for 5 min, 46 ◦C for 20 min, and 95 ◦C for 5 min. Quantitative PCR was performed using SYBR Green Supermix (Bio-Rad, Cat. No. 171–5121) on the QuantStudio 5 Real-Time PCR System. .. The expression levels of M1 and M2 markers (CD206, CD163, ARG1, iNOS and CD86) whereas EMT-related genes (E-cadherin, N-cadherin, Snail, Slug, MMP-2, Twist, Vimentin, and ZEB) were analysed, with GAPDH serving as the endogenous control.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
    Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).

    Single Cell RNA-Seq:

    Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
    Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).

    Gene Expression:

    Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
    Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).

    RNA Sequencing:

    Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
    Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).

    Sequencing:

    Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
    Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).

    Software:

    Article Title: Aire is a cell-intrinsic regulator of natural IgM-secreting cells.
    Article Snippet: Flowjo 10 (Trees- tar) software was used for analysis. .. REAGENT or RESOURCE SOURCE IDENTIFIER iScript cDNA Synthesis BioRad Cat# 1708890 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444557 NEBNext Ultra II New England Biolabs Cat# E7103L OneStep Ahead RT- PCR kit Qiagen Cat# 220211 Deposited data Single cell RNA-seq analysis of mature thymic epithelial cells Sansom et al. GEO: GSE53111 Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells St. Pierre et al. GEO: GSE65617 RNA sequencing of Aire-reporter-positive CD138+IgM+ cells from Aire+/+ and Aire–/– Aire-HCO mice This paper GEO: GSE310317 Bulk immunoglobulin heavy-chain repertoire sequencing This paper GEO: GSE332886 Experimental models: Organisms/strains Mus musculus (BALB/c): Aire- HCOTG: Aire-HCO4 Aschenbrenner et al.81 N/A Mus musculus (C57BL/6): AdigTG: Tg(Aire-G6pc2/GFP)1Mand Gardner et al.18 N/A Mus musculus (C57BL/6): Tcra -/-: Tcratm1Mom Mombaerts et al.82 N/A Mus musculus (C57BL/6): Cd40-/-: B6.129P2-Cd40tm1Kik/J Kawabe et al.83 N/A Mus musculus (C57BL/6): Airef/f: B6.Cg-Airetm1Dfil/J Dobes et al.52 N/A Mus musculus(BALB/c): Ciita-/-: C.129S2(B6)-Ciitatm1Ccum/J Chang et al.84 N/A Mus musculus (C57BL/6): Mb1-Cre: Cd79atm1(cre)Reth Hobeika et al.53 N/A Mus musculus (C57BL/6): Foxn1-Cre: Tg(Foxn1-cre)8Ghr Rossi et al.54 N/A Mus musculus (C57BL/6); Aire-/-; B6.129S2-Airetm1.1Doi Anderson et al.2 N/A Mus musculus (BALB/c): Aire-/-: C.Cg-Airetm1Pltn Ramsey et al.3 N/A Software and algorithms FlowJo software 10.7.2 Tree Star https://www.flowjo.com GraphPad PRISM 7.0 Graphpad Software https://www.graphpad.com/ NDP.view2 Hamamatsu Photonics https://www.hamamatsu.com/ Image J National Institute of Health https://imagej.net/ LEGENDplex Data Analysis Software Suite BioLegend https://www.biolegend.com/ ll OPEN ACCESS Article e3 Immunity 59, 1–19.e1–e6, December 8, 2026 .. Total RNA was isolated using the RNeasy RNA Isolation Kit (Qiagen).



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