Cell Culture:Article Title: High-throughput cytokine detection platform for evaluation of chemical induced microglial activation
Article Snippet: .. Human iPSC-derived differentiated microglia (catalog: BX-0900) were acquired from BrainXell (Madison, WI, USA) and cultured in poly- d -lysine (PDL)-coated plates using Advanced DMEM/F-12 medium (Thermo Fisher Scientific, catalog: 12,634,010, Waltham, MA, USA) supplemented with 1 % N-2 Supplement (Thermo Fisher Scientific, catalog: 17,502,048), 2 % B-27 Supplement (Thermo Fisher Scientific, catalog: 17,504,044), 1 % Glutamax Supplement (Thermo Fisher Scientific, catalog: 35,050,061), 1 % MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, catalog: 11,140,050), 1X Chemically Defined Lipid Concentrate (Thermo Fisher Scientific, catalog: 11,905,031), 0.2 mM Ascorbic Acid (Sigma-Aldrich, catalog: A8960), 100 ng/mL IL-34 (PeproTech, catalog: 200-34), 20 ng/mL MCSF (PeproTech, catalog: 300-25), 2 ng/mL TGF-β1 (PeproTech, catalog: 100-21), and 15 μg/mL Geltrex TM LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific, catalog: A1413201). .. The HMC3 human microglia cells (CRL-3304) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured with 90 % EMEM (ATCC, catalog: 30-2003) supplemented with 10 % fetal bovine serum (FBS, Cytiva, catalog: SH30070.03, Marlborough, MA, USA), as well as 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, catalog: 15,140,122).
Article Title: High-throughput cytokine detection platform for evaluation of chemical induced microglial activation.
Article Snippet: .. Human iPSC-derived differentiated microglia (catalog: BX-0900) were acquired from BrainXell (Madison, WI, USA) and cultured in poly-D-lysine (PDL)-coated plates using Advanced DMEM/F-12 medium (Thermo Fisher Scientific, catalog: 12,634,010, Waltham, MA, USA) supplemented with 1 % N-2 Supplement (Thermo Fisher Scientific, catalog: 17,502,048), 2 % B-27 Supplement (Thermo Fisher Scientific, catalog: 17,504,044), 1 % Glutamax Supplement (Thermo Fisher Scientific, catalog: 35,050,061), 1 % MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, catalog: 11,140,050), 1X Chemically Defined Lipid Concentrate (Thermo Fisher Scientific, catalog: 11,905,031), 0.2 mM Ascorbic Acid (Sigma-Aldrich, catalog: A8960), 100 ng/mL IL-34 (PeproTech, catalog: 200–34), 20 ng/mL MCSF (PeproTech, catalog: 300–25), 2 ng/mL TGF-β1 (PeproTech, catalog: 100–21), and 15 μg/mL GeltrexTM LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific, catalog: A1413201). .. The HMC3 human microglia cells (CRL-3304) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured with 90 % EMEM (ATCC, catalog: 30–2003) supplemented with 10 % fetal bovine serum (FBS, Cytiva, catalog: SH30070.03, Marlborough, MA, USA), as well as 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, catalog: 15,140,122).
Membrane:Article Title: High-throughput cytokine detection platform for evaluation of chemical induced microglial activation
Article Snippet: .. Human iPSC-derived differentiated microglia (catalog: BX-0900) were acquired from BrainXell (Madison, WI, USA) and cultured in poly- d -lysine (PDL)-coated plates using Advanced DMEM/F-12 medium (Thermo Fisher Scientific, catalog: 12,634,010, Waltham, MA, USA) supplemented with 1 % N-2 Supplement (Thermo Fisher Scientific, catalog: 17,502,048), 2 % B-27 Supplement (Thermo Fisher Scientific, catalog: 17,504,044), 1 % Glutamax Supplement (Thermo Fisher Scientific, catalog: 35,050,061), 1 % MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, catalog: 11,140,050), 1X Chemically Defined Lipid Concentrate (Thermo Fisher Scientific, catalog: 11,905,031), 0.2 mM Ascorbic Acid (Sigma-Aldrich, catalog: A8960), 100 ng/mL IL-34 (PeproTech, catalog: 200-34), 20 ng/mL MCSF (PeproTech, catalog: 300-25), 2 ng/mL TGF-β1 (PeproTech, catalog: 100-21), and 15 μg/mL Geltrex TM LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific, catalog: A1413201). .. The HMC3 human microglia cells (CRL-3304) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured with 90 % EMEM (ATCC, catalog: 30-2003) supplemented with 10 % fetal bovine serum (FBS, Cytiva, catalog: SH30070.03, Marlborough, MA, USA), as well as 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, catalog: 15,140,122).
Article Title: High-throughput cytokine detection platform for evaluation of chemical induced microglial activation.
Article Snippet: .. Human iPSC-derived differentiated microglia (catalog: BX-0900) were acquired from BrainXell (Madison, WI, USA) and cultured in poly-D-lysine (PDL)-coated plates using Advanced DMEM/F-12 medium (Thermo Fisher Scientific, catalog: 12,634,010, Waltham, MA, USA) supplemented with 1 % N-2 Supplement (Thermo Fisher Scientific, catalog: 17,502,048), 2 % B-27 Supplement (Thermo Fisher Scientific, catalog: 17,504,044), 1 % Glutamax Supplement (Thermo Fisher Scientific, catalog: 35,050,061), 1 % MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, catalog: 11,140,050), 1X Chemically Defined Lipid Concentrate (Thermo Fisher Scientific, catalog: 11,905,031), 0.2 mM Ascorbic Acid (Sigma-Aldrich, catalog: A8960), 100 ng/mL IL-34 (PeproTech, catalog: 200–34), 20 ng/mL MCSF (PeproTech, catalog: 300–25), 2 ng/mL TGF-β1 (PeproTech, catalog: 100–21), and 15 μg/mL GeltrexTM LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Thermo Fisher Scientific, catalog: A1413201). .. The HMC3 human microglia cells (CRL-3304) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured with 90 % EMEM (ATCC, catalog: 30–2003) supplemented with 10 % fetal bovine serum (FBS, Cytiva, catalog: SH30070.03, Marlborough, MA, USA), as well as 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, catalog: 15,140,122).
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