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iplex massarray analysis  (agena bioscience)


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    agena bioscience iplex massarray analysis
    <t> iPLEX MassArray </t> confirmation of the 7 DMRs identified with 450K array in FLEHS1 saliva samples
    Iplex Massarray Analysis, supplied by agena bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iplex+massarray+analysis/multiplexed+massarray+iplex+assay/pmc05896137-85-12-16
    Average 90 stars, based on 1 article reviews
    iplex massarray analysis - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "GLI2 promoter hypermethylation in saliva of children with a respiratory allergy"

    Article Title: GLI2 promoter hypermethylation in saliva of children with a respiratory allergy

    Journal: Clinical Epigenetics

    doi: 10.1186/s13148-018-0484-1

     iPLEX MassArray  confirmation of the 7 DMRs identified with 450K array in FLEHS1 saliva samples
    Figure Legend Snippet: iPLEX MassArray confirmation of the 7 DMRs identified with 450K array in FLEHS1 saliva samples

    Techniques Used: Control

     iPLEX MassArray  verification of the seven identified DMRs in the independent birth cohort FLEHS2
    Figure Legend Snippet: iPLEX MassArray verification of the seven identified DMRs in the independent birth cohort FLEHS2

    Techniques Used: Control

    Related Articles

    Genotyping:

    Article Title: Development and validation of a multivariable genotype-informed gestational diabetes prediction algorithm for clinical use in the Mexican population: insights into susceptibility mechanisms
    Article Snippet: Genomic DNA was extracted from EDTA-stabilized blood samples taken during the OGTT using the Maxwell RSC instrument (Promega, Dubendorf, Switzerland). .. Genotyping was performed by iPLEX MassARRAY PCR using the Agena platform (Agena Bioscience, San Diego, California, USA). .. iPLEX MassARRAY PCR and extension primers were designed from sequences containing each target SNP and 150 upstream and downstream bases with AssayDesign Suite software ( http://agenabio.com/assay-design-suite-20-software ) using the default settings.

    Polymerase Chain Reaction:

    Article Title: Development and validation of a multivariable genotype-informed gestational diabetes prediction algorithm for clinical use in the Mexican population: insights into susceptibility mechanisms
    Article Snippet: Genomic DNA was extracted from EDTA-stabilized blood samples taken during the OGTT using the Maxwell RSC instrument (Promega, Dubendorf, Switzerland). .. Genotyping was performed by iPLEX MassARRAY PCR using the Agena platform (Agena Bioscience, San Diego, California, USA). .. iPLEX MassARRAY PCR and extension primers were designed from sequences containing each target SNP and 150 upstream and downstream bases with AssayDesign Suite software ( http://agenabio.com/assay-design-suite-20-software ) using the default settings.

    Article Title: Maternal genomic profile, gestational diabetes control, and Mediterranean diet to prevent low birth weight
    Article Snippet: Genomic DNA was extracted from EDTA-stabilized blood samples, taken between 08.00 and 09.00 a.m. after an overnight fast, at the time of the OGTT for screening of GDM, between 12 and 14 GW (Visit 1), using the Maxwell RSC instrument (Promega, Dubendorf, Switzerland). .. Genotyping was performed by IPLEX MassARRAY PCR using the Agena platform (Agena Bioscience, SanDiego, CA). .. An Agena Bioscience Compact MassArray Spectrometer was used to perform MALDI-TOF mass spectrometry according to the iPLEX Gold Application Guide.

    Article Title: Genetic Variants in Folate and Cobalamin Metabolism-Related Genes in Pregnant Women of a Homogeneous Spanish Population: The Need for Revisiting the Current Vitamin Supplementation Strategies
    Article Snippet: Genomic DNA was extracted from the buccal swab using a MagMax DNA Multi-Sample Ultra Kit (Applied biosystems by Thermo Fisher Scientific, Waltham, MA, USA). .. Genotyping was performed via IPLEX MassARRAY PCR using the Agena platform (Agena Bioscience, San Diego, CA, USA). .. IPLEX MassARRAY PCR and extension primers were designed from sequences containing each target SNP and 150 upstream and downstream bases with Assay Designer 4.0.0.2 (Agena Bioscience, San Diego, CA, USA) using the default settings.

    Article Title: Genetic Variants in Folate and Cobalamin Metabolism-Related Genes in Pregnant Women of a Homogeneous Spanish Population: The Need for Revisiting the Current Vitamin Supplementation Strategies
    Article Snippet: Genotyping was performed via IPLEX MassARRAY PCR using the Agena platform (Agena Bioscience, San Diego, CA, USA). .. IPLEX MassARRAY PCR and extension primers were designed from sequences containing each target SNP and 150 upstream and downstream bases with Assay Designer 4.0.0.2 (Agena Bioscience, San Diego, CA, USA) using the default settings. .. Single base extension reactions were performed on the PCR reactions with the iPLEX Gold Kit (Agena Bioscience) and 0.8 μL of the custom UEP pool.

    Clinical Proteomics:

    Article Title: The associations between individual plasma SFAs, serine palmitoyl-transferase long-chain base subunit 3 gene rs680379 polymorphism, and type 2 diabetes among Chinese adults.
    Article Snippet: Background: Several individual studies have shown that circulating levels of odd-chain SFAs and very-long-chain SFAs (VLSFAs) may have beneficial effects, but the results are mixed.. While the dietary and metabolic factors that may influence VLSFAs are not well-known, a previous study observed associations of VLSFA concentrations with variants in serine palmitoyl-transferase longchain base subunit 3 (SPTLC3) gene.. Objectives: We investigated the associations of individual plasma SFAs and SPTLC3 gene rs680379 polymorphism with metabolic risk factors and type 2 diabetes (T2D).

    Gas Chromatography:

    Article Title: The associations between individual plasma SFAs, serine palmitoyl-transferase long-chain base subunit 3 gene rs680379 polymorphism, and type 2 diabetes among Chinese adults.
    Article Snippet: Background: Several individual studies have shown that circulating levels of odd-chain SFAs and very-long-chain SFAs (VLSFAs) may have beneficial effects, but the results are mixed.. While the dietary and metabolic factors that may influence VLSFAs are not well-known, a previous study observed associations of VLSFA concentrations with variants in serine palmitoyl-transferase longchain base subunit 3 (SPTLC3) gene.. Objectives: We investigated the associations of individual plasma SFAs and SPTLC3 gene rs680379 polymorphism with metabolic risk factors and type 2 diabetes (T2D).

    other:

    Article Title: Exome sequencing identified rare recurrent copy number variants and hereditary breast cancer susceptibility
    Article Snippet: The genotyping of the polymorphic 3 ́ end PTVs in RAD52 (rs4987207 and rs4987208) was performed with HRM analysis and iPLEX (Agena Bioscience MassARRAY System, Sequenom Inc., FIMM).

    Article Title: Effect of pharmacogenomic testing on pediatric mental health outcome: a 6-month follow-up.
    Article Snippet: Ahmed Z Elmaadawi*,1 , Rikin Patel2, Yarob Almaaitah3 & Matthew G Logsdon4 1Department of Psychiatry, Indiana University School of Medicine–South Bend Campus, Beacon Health System, 707 N. Michigan Street, Suite 400, South Bend, IN 46601, USA 2Department of Child & Adolescent Psychiatry, Duke University Medical Center, Durham, NC 27708, USA 3Department of Psychiatry, Beacon Medical Group Behavioral Health, 707 N. Michigan Street, South Bend, IN 46601, USA 4Internal Medicine Program, University of Miami Miller School of Medicine, 1600 NW 10th Ave #1140, Miami, FL 33136, USA *Author for correspondence: aelmaada@iu.edu



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    Image Search Results


     MassARRAY  primers

    Journal: BMC Medical Genomics

    Article Title: MassARRAY multigene screening combined with LDL-C and sdLDL-C detection for more favorable outcomes in type 2 diabetes mellitus therapy

    doi: 10.1186/s12920-021-00937-8

    Figure Lengend Snippet: MassARRAY primers

    Article Snippet: The MassARRAY iPLEX Gold multiple genotyping analysis system (Agena Bioscience, Inc.) was used, and the reagents contained Agena PCR reagent, Agena SAP reagent, and Agena iPLEX reagent.

    Techniques: Sequencing

    Participant information for  MassARRAY  SNP detection

    Journal: Translational Cancer Research

    Article Title: Noninvasive circulating tumor cell and urine cellular XPC (rs2228001, A2815C) and XRCC1 (rs25487, G1196A) polymorphism detection as an effective screening panel for genitourinary system cancers

    doi: 10.21037/tcr.2019.10.47

    Figure Lengend Snippet: Participant information for MassARRAY SNP detection

    Article Snippet: MassARRAY iPLEX Gold multiple genotyping analysis (Agena Bioscience, Inc.) reagents (Agena PCR reagent, Agena SAP reagent, and Agena iPLEX reagent) were provided by Agena Bioscience.

    Techniques:

    Participant information for both CTC and  MassARRAY  SNP detection

    Journal: Translational Cancer Research

    Article Title: Noninvasive circulating tumor cell and urine cellular XPC (rs2228001, A2815C) and XRCC1 (rs25487, G1196A) polymorphism detection as an effective screening panel for genitourinary system cancers

    doi: 10.21037/tcr.2019.10.47

    Figure Lengend Snippet: Participant information for both CTC and MassARRAY SNP detection

    Article Snippet: MassARRAY iPLEX Gold multiple genotyping analysis (Agena Bioscience, Inc.) reagents (Agena PCR reagent, Agena SAP reagent, and Agena iPLEX reagent) were provided by Agena Bioscience.

    Techniques:

     iPLEX MassArray  confirmation of the 7 DMRs identified with 450K array in FLEHS1 saliva samples

    Journal: Clinical Epigenetics

    Article Title: GLI2 promoter hypermethylation in saliva of children with a respiratory allergy

    doi: 10.1186/s13148-018-0484-1

    Figure Lengend Snippet: iPLEX MassArray confirmation of the 7 DMRs identified with 450K array in FLEHS1 saliva samples

    Article Snippet: Differentially methylated CpG sites in the identified DMRs were further confirmed using iPLEX MassArray analysis by Agena Bioscience (Hamburg, Germany) according to the manufacturer’s protocol (iPLEX® Pro Application Guide, SQNM-USG-CUS-030 Rev 3.0; CO-12-274, 2012).

    Techniques: Control

     iPLEX MassArray  verification of the seven identified DMRs in the independent birth cohort FLEHS2

    Journal: Clinical Epigenetics

    Article Title: GLI2 promoter hypermethylation in saliva of children with a respiratory allergy

    doi: 10.1186/s13148-018-0484-1

    Figure Lengend Snippet: iPLEX MassArray verification of the seven identified DMRs in the independent birth cohort FLEHS2

    Article Snippet: Differentially methylated CpG sites in the identified DMRs were further confirmed using iPLEX MassArray analysis by Agena Bioscience (Hamburg, Germany) according to the manufacturer’s protocol (iPLEX® Pro Application Guide, SQNM-USG-CUS-030 Rev 3.0; CO-12-274, 2012).

    Techniques: Control