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phase contrast microscope  (Nikon)


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    Structured Review

    Nikon phase contrast microscope
    Phase Contrast Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+phase+microscope/Inverted+Microscopes/pm41920403-46-26-28
    Average 99 stars, based on 14654 article reviews
    phase contrast microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. Fluorescence images were captured using a Nikon inverted fluorescence microscope, and the MitoSOX Red fluorescence intensity was quantitatively analyzed using NIS-Elements AR software. ..

    Article Title: Distribution and functional significance of rodent cerebellar glycogen
    Article Snippet: .. Epifluorescence images of coronal or sagittal sections were acquired using an inverted fluorescence microscope (Nikon ECLIPSE Ni-E, Mono-Camera Nikon DS-Fi3) controlled by proprietary software (NIS-Elements Imaging AR 4.60.00). .. Confocal images were acquired with a Nikon Eclipse Ti2 microscope (NIS-Elements AR 4.50.00) with a Plan Apo x60/1.40 numerical aperture (NA) oil-immersion objective at a Z step of 1 μm.

    Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula
    Article Snippet: .. An inverted fluorescence microscope (Nikon, TiS, Japan) was applied to capture images. ..

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: The next day, after three PBS washes, fluorescently labeled secondary antibodies (Alexa Fluor 488 or 594, 1:500; Abcam) were added and incubated in the dark at room temperature for 1 h. Unbound secondary antibodies were washed away, and cell nuclei were stained with DAPI for 5 min. .. Finally, the cells were washed three times with PBS, mounted with anti-fluorescence quenching medium, and fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure data accuracy and reproducibility.

    Microscopy:

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. Fluorescence images were captured using a Nikon inverted fluorescence microscope, and the MitoSOX Red fluorescence intensity was quantitatively analyzed using NIS-Elements AR software. ..

    Article Title: Structural Color Inkjet Printing With Mie-Resonant Silicon Nanoparticles.
    Article Snippet: Elemental mapping was performed using scanning transmission electron microscopy (STEM; JEM-2100F, JEOL) equipped with an energy-dispersive X-ray spectrometer (EDX; JED-2300, JEOL). .. Reflectance and transmittance spectra were measured via microspectroscopy using an inverted optical microscope (Ti-U, Nikon). ..

    Article Title: Distribution and functional significance of rodent cerebellar glycogen
    Article Snippet: .. Epifluorescence images of coronal or sagittal sections were acquired using an inverted fluorescence microscope (Nikon ECLIPSE Ni-E, Mono-Camera Nikon DS-Fi3) controlled by proprietary software (NIS-Elements Imaging AR 4.60.00). .. Confocal images were acquired with a Nikon Eclipse Ti2 microscope (NIS-Elements AR 4.50.00) with a Plan Apo x60/1.40 numerical aperture (NA) oil-immersion objective at a Z step of 1 μm.

    Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula
    Article Snippet: .. An inverted fluorescence microscope (Nikon, TiS, Japan) was applied to capture images. ..

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: The next day, after three PBS washes, fluorescently labeled secondary antibodies (Alexa Fluor 488 or 594, 1:500; Abcam) were added and incubated in the dark at room temperature for 1 h. Unbound secondary antibodies were washed away, and cell nuclei were stained with DAPI for 5 min. .. Finally, the cells were washed three times with PBS, mounted with anti-fluorescence quenching medium, and fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure data accuracy and reproducibility.

    Software:

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. Fluorescence images were captured using a Nikon inverted fluorescence microscope, and the MitoSOX Red fluorescence intensity was quantitatively analyzed using NIS-Elements AR software. ..

    Article Title: Distribution and functional significance of rodent cerebellar glycogen
    Article Snippet: .. Epifluorescence images of coronal or sagittal sections were acquired using an inverted fluorescence microscope (Nikon ECLIPSE Ni-E, Mono-Camera Nikon DS-Fi3) controlled by proprietary software (NIS-Elements Imaging AR 4.60.00). .. Confocal images were acquired with a Nikon Eclipse Ti2 microscope (NIS-Elements AR 4.50.00) with a Plan Apo x60/1.40 numerical aperture (NA) oil-immersion objective at a Z step of 1 μm.

    Incubation:

    Article Title: MS4322 is a selective protein arginine methyltransferase 5 degrader with antitumor effects in cervical cancer cells
    Article Snippet: The cell monolayer was scraped with a 10 μl pipette tip to produce a wound, washed with PBS to remove detached cells and the cells were incubated in serum-free DMEM (Gibco; Thermo Fisher Scientific, Inc.). .. Cells were incubated at 37°C for 24 h. Photographs were taken at 0 and 24 h using a Nikon inverted light microscope (Nikon Instruments). .. Wound width analysis was performed with Image-Pro Plus 6.0 software (Media Cybernetics, Inc.).

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.

    Light Microscopy:

    Article Title: MS4322 is a selective protein arginine methyltransferase 5 degrader with antitumor effects in cervical cancer cells
    Article Snippet: The cell monolayer was scraped with a 10 μl pipette tip to produce a wound, washed with PBS to remove detached cells and the cells were incubated in serum-free DMEM (Gibco; Thermo Fisher Scientific, Inc.). .. Cells were incubated at 37°C for 24 h. Photographs were taken at 0 and 24 h using a Nikon inverted light microscope (Nikon Instruments). .. Wound width analysis was performed with Image-Pro Plus 6.0 software (Media Cybernetics, Inc.).

    Staining:

    Article Title: Histone H3K9 lactylation activates the TXNIP/NLRP3 pathway to drive macrophage inflammation after spinal cord injury
    Article Snippet: Sections were stained with a H&E Staining Kit (Beyotime, C0105S) according to the manufacturer's instructions. .. Stained sections were imaged on a Nikon inverted microscope and analyzed using ImageJ. ..

    Inverted Microscopy:

    Article Title: Histone H3K9 lactylation activates the TXNIP/NLRP3 pathway to drive macrophage inflammation after spinal cord injury
    Article Snippet: Sections were stained with a H&E Staining Kit (Beyotime, C0105S) according to the manufacturer's instructions. .. Stained sections were imaged on a Nikon inverted microscope and analyzed using ImageJ. ..

    Imaging:

    Article Title: Distribution and functional significance of rodent cerebellar glycogen
    Article Snippet: .. Epifluorescence images of coronal or sagittal sections were acquired using an inverted fluorescence microscope (Nikon ECLIPSE Ni-E, Mono-Camera Nikon DS-Fi3) controlled by proprietary software (NIS-Elements Imaging AR 4.60.00). .. Confocal images were acquired with a Nikon Eclipse Ti2 microscope (NIS-Elements AR 4.50.00) with a Plan Apo x60/1.40 numerical aperture (NA) oil-immersion objective at a Z step of 1 μm.



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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Image Search Results


    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain

    Journal: BMC Oral Health

    Article Title: Dose-dependent effects of Metformin on proliferation and odontogenic differentiation of dental pulp stem cells

    doi: 10.1186/s12903-026-08221-w

    Figure Lengend Snippet: Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain

    Article Snippet: Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14.

    Techniques: Staining, Cell Culture, Control, Microscopy, Software, Derivative Assay