interactive spindle photoconversion analysis gui matlab 2020b (MathWorks Inc)
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Interactive Spindle Photoconversion Analysis Gui Matlab 2020b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/interactive+gui/pmc09398449-6-3-8
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Self-organization of kinetochore-fibers in human mitotic spindles"
Article Title: Self-organization of kinetochore-fibers in human mitotic spindles
Journal: eLife
doi: 10.7554/eLife.75458
Figure Legend Snippet: ( A ) Photoactivation experiment showing PA-GFP:alpha-tubulin and SNAP-SIR:centrin immediately preceding photoactivation, 0 s, 30 s, and 60 s after photoactivation with a 750nm femtosecond pulsed laser; 500ms 488nm excitation, 514/30 bandpass emission filter; 300ms 647nm excitation, 647 longpass emission filter; 5s frame rate. ( B ) Line profile generated by averaging the intensity in 15 pixels on either side of the spindle axis in the dotted box shown in A. The intensity is corrected for background from the opposite side of the spindle (see methods). ( C ) Line profiles (shades of green) fit to Gaussian profiles (shades of grey) at 0s, 5s and 25s. Lighter shades are earlier times. The solid line on the fit represents the fit pixels. ( D ) Blue dots: fit position of the line profile peak from the sample cell shown in A, B, and C over time. Black line: linear fit to the central position of the fit peak over time. ( E ) Red dots: fit height of the line profile peak from the sample cell shown in A, B, and C over time. Black line: dual-exponential fit to the fit height of the peak over time. ( F ) Sample ultrastructure from a 3D spindle reconstructed by electron tomography . KMTs are shown in red, non-KMTs yellow. ( G ) Comparison between the mean slow fraction from the photoconversion data (26% ± 2%, n=52 cells, error bars are standard error of the mean) and the fraction of KMTs (25% ± 2%, n=3 cells, error bars are standard error of the mean) from the EM data. The two means are statistically indistinguishable with P =0.86 on a Student’s t-test.
Techniques Used: Generated, Tomography, Comparison
Figure Legend Snippet: ( A ) Sample simulated images and line profiles from a photoconversion simulation using KMT minus end speeds in the nucleate at kinetochore model. ( B ) Comparison of the predicted spatial dependence tubulin flux speed in the nucleate at kinetochore and capture from spindle models. Error bars are standard error of the mean. ( C ) Relative probabilities of hybrid version of the two models.
Techniques Used: Comparison
Figure Legend Snippet: ( A ) Sample simulated images and line profiles from a photoconversion simulation using KMT minus end speeds in the nucleate at kinetochore model. ( B ) Sample simulated images and line profiles from a photoconversion simulation using KMT minus end speeds in the capture from spindle model. ( C ) Comparison of the predicted spatial dependence tubulin speed in the nucleate at kinetochore and capture from spindle models. Error bars are standard error of the mean.
Techniques Used: Comparison
Figure Legend Snippet: Parameters values and sources.
Techniques Used: Electron Microscopy
Figure Legend Snippet:
Techniques Used: Transfection, Construct, Labeling, Retroviral, Plasmid Preparation, Selection, Marker, Software, Control, Imaging, Light Microscopy
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![The “Preprocessing” pop-up window is the initial screen of Robin’s Viewer (RV) and has five distinct sections: First, the electroencephalography (EEG)-file selection (see Section “3.1 Loading EEG data”), where the user can load their EEG recording and display selected statistics about the data once it is loaded. Second, the preprocessing settings (see Section “3.2 Preprocessing and visualization settings”), which are used to set a bandpass filter [finite impulse response (FIR) filter with Blackman window] and a custom reference. Third, the bad-channel handling (see Sections “3.2 Preprocessing and visualization settings” and “3.4 Bad-channel marking”), where the user can decide whether to use automatic bad channel detection, and whether to interpolate bad channels. Fourth, the visualization settings (see Section “3.2 Preprocessing and visualization settings”), comprised of downsampling, custom scaling (by default 1e-6 as RV scales data from volts to microvolts for plotting), the gap between traces (by default 40 (μV); setting this to 0 results in butterfly mode where all traces are collapsed on top of each other; values higher than 40 move traces further apart), segment length to plot [by default 60 (seconds)], whether or not to activate the view-slider, and selection of channels to plot. Visualization settings will only be applied to the data for plotting and hence will not be saved in the save-file (in contrast to the preprocessing settings). Fifth, the deep-learning model settings (see Sections “3.2 Preprocessing and visualization settings” and 3.5 Deep-learning model predictions”), where previously saved model output can be loaded and the integrated deep-learning model can be activated to generate predictions. Clicking the “Plot” button at the bottom will close this window and, after a loading screen (which lasts as long as it takes to plot the data), it will open up the main <t>graphical</t> <t>user</t> <t>interface</t> <t>(GUI).</t>](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1202/pmc09951202/pmc09951202__fninf-16-1025847-g001.jpg)