Journal: iScience

Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

doi: 10.1016/j.isci.2025.114190

Figure Lengend Snippet: The N- and C-termini dependency for TCTN1 in the transition zone localization (A) Immunostaining images showing the ciliary base signals using HA antibody (green) in WT, tctn1 , TCTN1, DUF1619, ΔDUF1619, N-DUF1619, and DUF1619-C cell lines. The signal of acetylated α-tubulin (Ac-tubulin, red) marks the cilium. The white arrowheads mark the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone puncta. The statistical analysis of the percentage of fluorescence localization at the TZ is also presented ( n = 17, 19, 25, 35, 26, 41, and 41). Scale bars, 5 μm.

Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

Techniques: Immunostaining, Fluorescence

Journal: iScience

Article Title: Profiling truncated variants of TCTN1 unveils the essential role of its integrity for ciliogenesis

doi: 10.1016/j.isci.2025.114190

Figure Lengend Snippet: The transition zone localizations of NPHP4 or NPHP8 in truncated TCTN1 mutants are not altered (A and B) Immunostaining images displaying the ciliary base puncta of NPHP4 (A) or NPHP8 (B) in WT, tctn1 ::TCTN1-HA (TCTN1), and tctn1 ::TCTN1(DUF1619)-HA (DUF1619) cells. All the cells were immunostained with anti-NPHP4 (green) or anti-NPHP8 (green) and anti-acetylated α-tubulin (Ac-tubulin, red) antibodies. The nuclei were stained with DAPI (blue). The white arrowheads indicate the ciliary base (ciliary transition zone). The insets show higher magnification views of the transition zone region. The brightfield (BF) and merge channels are also showed. Scale bars, 5 μm. See also for other truncated strains.

Article Snippet: Immunostaining Blocking/Primary Antibody Dilution Solution , Sangon Biotech , Cat#E674004.

Techniques: Immunostaining, Staining

Journal: Materials Today Bio

Article Title: A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis

doi: 10.1016/j.mtbio.2025.102009

Figure Lengend Snippet: The impact of Tet2 knockdown on DPA@NM@CTZE expression. ( A-B ) It shows IHC images of LC3B, OCN, and RUNX2. Scale bar: 400 μm. (C-D ) It displays calcein double-standard images and analysis. Scale bar: 100 μm. Results are expressed as Mean ± SD, with significance denoted by ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, n = 5.

Article Snippet: Subsequently, 1 mL of pre-cooled methanol was added, and the samples were placed in a −20 °C refrigerator for 10 min. After this step, the samples were washed 3 times with pre-cooled PBS, each wash lasting 5 min. Triton X-100 (1 mL) was then added for 10 min, and the samples were incubated with an Immunostaining blocking solution (Beyotime, China).

Techniques: Knockdown, Expressing